Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3278 and GFF3279 are separated by 101 nucleotides GFF3279 and GFF3280 are separated by 342 nucleotides GFF3280 and GFF3281 are separated by 88 nucleotides
GFF3277 - Membrane protein insertion efficiency factor YidD, at 98,035 to 98,322
GFF3277
GFF3278 - Ribonuclease P protein component (EC 3.1.26.5), at 98,319 to 98,741
GFF3278
GFF3279 - LSU ribosomal protein L34p, at 98,843 to 98,977
GFF3279
GFF3280 - Chromosomal replication initiator protein DnaA, at 99,320 to 100,699
GFF3280
GFF3281 - DNA polymerase III beta subunit (EC 2.7.7.7), at 100,788 to 101,894
GFF3281
Position (kb)
99
100
101 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 99.234 kb on - strand at 99.234 kb on - strand at 99.234 kb on - strand at 99.234 kb on - strand at 99.234 kb on - strand at 99.234 kb on - strand at 99.234 kb on - strand at 99.271 kb on + strand at 99.271 kb on + strand at 99.271 kb on + strand at 99.271 kb on + strand at 99.275 kb on + strand at 99.275 kb on + strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.276 kb on - strand at 99.277 kb on + strand at 99.277 kb on + strand at 100.749 kb on + strand at 100.749 kb on + strand at 100.749 kb on + strand at 100.749 kb on + strand at 100.749 kb on + strand at 100.749 kb on + strand at 100.749 kb on + strand at 100.749 kb on + strand at 100.749 kb on + strand at 100.750 kb on - strand at 100.750 kb on - strand at 100.750 kb on - strand at 100.750 kb on - strand at 100.769 kb on + strand at 100.769 kb on + strand at 100.769 kb on + strand at 100.769 kb on + strand at 100.769 kb on + strand at 100.769 kb on + strand at 100.769 kb on + strand at 100.769 kb on + strand at 100.769 kb on + strand at 100.769 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 99,234 - -0.2 99,234 - +0.8 99,234 - -0.3 99,234 - -0.1 99,234 - +0.4 99,234 - +0.5 99,234 - +0.4 99,271 + +0.3 99,271 + -1.0 99,271 + -0.5 99,271 + -0.7 99,275 + -0.7 99,275 + +2.1 99,276 - +0.8 99,276 - +0.8 99,276 - -1.0 99,276 - +0.4 99,276 - +1.1 99,276 - -1.3 99,276 - +1.1 99,276 - -0.6 99,276 - +1.1 99,276 - +0.3 99,276 - +0.8 99,276 - +0.8 99,277 + -0.5 99,277 + -1.0 100,749 + +0.4 100,749 + -0.2 100,749 + +0.3 100,749 + -1.9 100,749 + -2.2 100,749 + -0.4 100,749 + -0.0 100,749 + -0.4 100,749 + -0.2 100,750 - -1.6 100,750 - -0.2 100,750 - -0.7 100,750 - +1.1 100,769 + -0.4 100,769 + +0.1 100,769 + -0.6 100,769 + -0.5 100,769 + +0.4 100,769 + -0.3 100,769 + -0.7 100,769 + -0.5 100,769 + +0.1 100,769 + -0.9
Or see this region's nucleotide sequence