Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3275 and GFF3276 are separated by 75 nucleotides GFF3276 and GFF3277 overlap by 4 nucleotides GFF3277 and GFF3278 overlap by 4 nucleotides GFF3278 and GFF3279 are separated by 101 nucleotides
GFF3275 - tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmE, at 94,871 to 96,277
GFF3275
GFF3276 - Inner membrane protein translocase and chaperone YidC, long form, at 96,353 to 98,038
GFF3276
GFF3277 - Membrane protein insertion efficiency factor YidD, at 98,035 to 98,322
GFF3277
GFF3278 - Ribonuclease P protein component (EC 3.1.26.5), at 98,319 to 98,741
GFF3278
GFF3279 - LSU ribosomal protein L34p, at 98,843 to 98,977
GFF3279
Position (kb)
96
97
98
99 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 96.129 kb on + strand, within GFF3275 at 96.130 kb on - strand, within GFF3275 at 96.130 kb on - strand, within GFF3275 at 96.309 kb on + strand at 96.310 kb on - strand at 96.310 kb on - strand at 96.310 kb on - strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.354 kb on + strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 96.355 kb on - strand at 98.180 kb on + strand, within GFF3277 at 98.180 kb on + strand, within GFF3277 at 98.180 kb on + strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.181 kb on - strand, within GFF3277 at 98.226 kb on - strand, within GFF3277 at 98.288 kb on + strand, within GFF3277 at 98.289 kb on - strand, within GFF3277 at 98.289 kb on - strand, within GFF3277 at 98.289 kb on - strand, within GFF3277 at 98.289 kb on - strand, within GFF3277
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 96,129 + GFF3275 0.89 +1.8 96,130 - GFF3275 0.89 +1.8 96,130 - GFF3275 0.89 -0.2 96,309 + -0.9 96,310 - -0.9 96,310 - +2.4 96,310 - +0.0 96,354 + -0.6 96,354 + -0.1 96,354 + -0.2 96,354 + +0.2 96,354 + +0.2 96,354 + -0.2 96,354 + +2.3 96,354 + -0.5 96,354 + +0.1 96,354 + -0.5 96,354 + -0.3 96,354 + +0.2 96,354 + -0.2 96,354 + -0.3 96,354 + -0.7 96,354 + -0.4 96,354 + +0.4 96,354 + -0.5 96,354 + -0.0 96,354 + -0.1 96,354 + +0.3 96,355 - -0.2 96,355 - -0.5 96,355 - -0.1 96,355 - +0.2 96,355 - -0.9 96,355 - -0.8 96,355 - +0.2 96,355 - +0.4 96,355 - +2.4 96,355 - -0.1 96,355 - +0.1 96,355 - -1.5 96,355 - -0.8 96,355 - -0.1 96,355 - -0.5 96,355 - -0.2 96,355 - -0.6 96,355 - -0.0 96,355 - +0.5 96,355 - -0.2 98,180 + GFF3277 0.50 -0.5 98,180 + GFF3277 0.50 -0.5 98,180 + GFF3277 0.50 -1.6 98,181 - GFF3277 0.51 +0.3 98,181 - GFF3277 0.51 -0.3 98,181 - GFF3277 0.51 -0.2 98,181 - GFF3277 0.51 +0.1 98,181 - GFF3277 0.51 +0.0 98,181 - GFF3277 0.51 -0.5 98,181 - GFF3277 0.51 +0.3 98,181 - GFF3277 0.51 -0.6 98,181 - GFF3277 0.51 -1.0 98,181 - GFF3277 0.51 -0.2 98,181 - GFF3277 0.51 -0.3 98,226 - GFF3277 0.66 -2.5 98,288 + GFF3277 0.88 -1.3 98,289 - GFF3277 0.88 -1.4 98,289 - GFF3277 0.88 +0.2 98,289 - GFF3277 0.88 -0.2 98,289 - GFF3277 0.88 -1.3
Or see this region's nucleotide sequence