Strain Fitness in Variovorax sp. SCN45 around GFF3276

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3275 and GFF3276 are separated by 75 nucleotidesGFF3276 and GFF3277 overlap by 4 nucleotidesGFF3277 and GFF3278 overlap by 4 nucleotidesGFF3278 and GFF3279 are separated by 101 nucleotides GFF3275 - tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmE, at 94,871 to 96,277 GFF3275 GFF3276 - Inner membrane protein translocase and chaperone YidC, long form, at 96,353 to 98,038 GFF3276 GFF3277 - Membrane protein insertion efficiency factor YidD, at 98,035 to 98,322 GFF3277 GFF3278 - Ribonuclease P protein component (EC 3.1.26.5), at 98,319 to 98,741 GFF3278 GFF3279 - LSU ribosomal protein L34p, at 98,843 to 98,977 GFF3279 Position (kb) 96 97 98 99Strain fitness (log2 ratio) -2 -1 0 1 2at 96.129 kb on + strand, within GFF3275at 96.130 kb on - strand, within GFF3275at 96.130 kb on - strand, within GFF3275at 96.309 kb on + strandat 96.310 kb on - strandat 96.310 kb on - strandat 96.310 kb on - strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.354 kb on + strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 96.355 kb on - strandat 98.180 kb on + strand, within GFF3277at 98.180 kb on + strand, within GFF3277at 98.180 kb on + strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.181 kb on - strand, within GFF3277at 98.226 kb on - strand, within GFF3277at 98.288 kb on + strand, within GFF3277at 98.289 kb on - strand, within GFF3277at 98.289 kb on - strand, within GFF3277at 98.289 kb on - strand, within GFF3277at 98.289 kb on - strand, within GFF3277

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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96,129 + GFF3275 0.89 +1.8
96,130 - GFF3275 0.89 +1.8
96,130 - GFF3275 0.89 -0.2
96,309 + -0.9
96,310 - -0.9
96,310 - +2.4
96,310 - +0.0
96,354 + -0.6
96,354 + -0.1
96,354 + -0.2
96,354 + +0.2
96,354 + +0.2
96,354 + -0.2
96,354 + +2.3
96,354 + -0.5
96,354 + +0.1
96,354 + -0.5
96,354 + -0.3
96,354 + +0.2
96,354 + -0.2
96,354 + -0.3
96,354 + -0.7
96,354 + -0.4
96,354 + +0.4
96,354 + -0.5
96,354 + -0.0
96,354 + -0.1
96,354 + +0.3
96,355 - -0.2
96,355 - -0.5
96,355 - -0.1
96,355 - +0.2
96,355 - -0.9
96,355 - -0.8
96,355 - +0.2
96,355 - +0.4
96,355 - +2.4
96,355 - -0.1
96,355 - +0.1
96,355 - -1.5
96,355 - -0.8
96,355 - -0.1
96,355 - -0.5
96,355 - -0.2
96,355 - -0.6
96,355 - -0.0
96,355 - +0.5
96,355 - -0.2
98,180 + GFF3277 0.50 -0.5
98,180 + GFF3277 0.50 -0.5
98,180 + GFF3277 0.50 -1.6
98,181 - GFF3277 0.51 +0.3
98,181 - GFF3277 0.51 -0.3
98,181 - GFF3277 0.51 -0.2
98,181 - GFF3277 0.51 +0.1
98,181 - GFF3277 0.51 +0.0
98,181 - GFF3277 0.51 -0.5
98,181 - GFF3277 0.51 +0.3
98,181 - GFF3277 0.51 -0.6
98,181 - GFF3277 0.51 -1.0
98,181 - GFF3277 0.51 -0.2
98,181 - GFF3277 0.51 -0.3
98,226 - GFF3277 0.66 -2.5
98,288 + GFF3277 0.88 -1.3
98,289 - GFF3277 0.88 -1.4
98,289 - GFF3277 0.88 +0.2
98,289 - GFF3277 0.88 -0.2
98,289 - GFF3277 0.88 -1.3

Or see this region's nucleotide sequence