Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3256 and GFF3257 are separated by 101 nucleotides GFF3257 and GFF3258 are separated by 101 nucleotides GFF3258 and GFF3259 overlap by 29 nucleotides GFF3259 and GFF3260 overlap by 4 nucleotides
GFF3256 - Peptidase, M20/M25/M40 family, at 74,347 to 75,702
GFF3256
GFF3257 - hypothetical protein, at 75,804 to 76,262
GFF3257
GFF3258 - hypothetical protein, at 76,364 to 79,288
GFF3258
GFF3259 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23), at 79,260 to 80,120
GFF3259
GFF3260 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 80,117 to 81,628
GFF3260
Position (kb) 76
77
78
79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 75.693 kb on + strand at 75.694 kb on - strand at 75.700 kb on + strand at 75.700 kb on + strand at 75.700 kb on + strand at 75.700 kb on + strand at 75.700 kb on + strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.747 kb on + strand at 75.747 kb on + strand at 75.747 kb on + strand at 75.748 kb on - strand at 75.748 kb on - strand at 75.748 kb on - strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.838 kb on + strand at 75.838 kb on + strand at 75.838 kb on + strand at 75.838 kb on + strand at 75.838 kb on + strand at 75.839 kb on - strand at 75.839 kb on - strand at 76.355 kb on + strand at 76.355 kb on + strand at 76.355 kb on + strand at 76.356 kb on - strand at 76.356 kb on - strand at 76.356 kb on - strand at 76.356 kb on - strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.559 kb on + strand at 76.560 kb on - strand at 76.560 kb on - strand at 76.560 kb on - strand at 76.560 kb on - strand at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.351 kb on + strand, within GFF3258 at 77.351 kb on + strand, within GFF3258 at 77.351 kb on + strand, within GFF3258 at 77.352 kb on - strand, within GFF3258 at 77.352 kb on - strand, within GFF3258 at 77.352 kb on - strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.417 kb on + strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.418 kb on - strand, within GFF3258 at 77.628 kb on - strand, within GFF3258 at 77.765 kb on + strand, within GFF3258 at 78.137 kb on + strand, within GFF3258 at 78.138 kb on - strand, within GFF3258 at 78.138 kb on - strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.712 kb on + strand, within GFF3258 at 78.712 kb on + strand, within GFF3258 at 78.712 kb on + strand, within GFF3258 at 78.713 kb on - strand, within GFF3258 at 78.713 kb on - strand, within GFF3258 at 78.990 kb on + strand, within GFF3258 at 78.991 kb on - strand, within GFF3258 at 79.348 kb on + strand, within GFF3259 at 79.348 kb on + strand, within GFF3259 at 79.348 kb on + strand, within GFF3259 at 79.348 kb on + strand, within GFF3259 at 79.349 kb on - strand, within GFF3259 at 79.451 kb on - strand, within GFF3259 at 79.510 kb on + strand, within GFF3259 at 79.510 kb on + strand, within GFF3259 at 79.511 kb on - strand, within GFF3259 at 79.511 kb on - strand, within GFF3259 at 79.570 kb on + strand, within GFF3259 at 79.570 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.604 kb on - strand, within GFF3259 at 79.604 kb on - strand, within GFF3259 at 79.604 kb on - strand, within GFF3259 at 79.604 kb on - strand, within GFF3259 at 79.972 kb on + strand, within GFF3259 at 79.973 kb on - strand, within GFF3259 at 80.209 kb on - strand at 80.226 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 75,693 + -0.6 75,694 - -0.2 75,700 + -0.3 75,700 + -0.3 75,700 + -0.2 75,700 + -0.5 75,700 + -0.2 75,701 - -1.3 75,701 - +0.0 75,701 - -0.6 75,701 - +0.0 75,701 - -0.7 75,701 - -1.2 75,701 - +0.8 75,747 + -0.3 75,747 + -0.6 75,747 + -0.9 75,748 - -0.7 75,748 - +0.1 75,748 - -0.1 75,763 + +0.0 75,763 + -0.0 75,763 + +0.1 75,763 + +0.0 75,763 + +0.3 75,763 + -2.1 75,763 + -0.5 75,763 + -0.6 75,763 + -0.1 75,763 + +0.3 75,763 + +0.1 75,763 + -0.1 75,763 + -0.2 75,763 + -0.4 75,763 + -0.6 75,763 + -0.1 75,763 + +0.4 75,764 - -0.4 75,764 - -0.0 75,764 - -0.7 75,764 - -0.2 75,764 - -0.2 75,764 - -0.3 75,838 + +1.0 75,838 + -0.1 75,838 + +1.8 75,838 + -0.3 75,838 + -0.2 75,839 - +1.1 75,839 - +0.7 76,355 + -0.2 76,355 + +0.4 76,355 + +0.2 76,356 - +0.3 76,356 - -0.1 76,356 - -0.3 76,356 - +1.0 76,389 + -0.1 76,389 + -0.4 76,389 + +0.7 76,389 + +0.0 76,389 + +0.4 76,389 + -2.2 76,389 + +0.4 76,389 + -0.6 76,390 - -1.4 76,390 - +0.0 76,390 - -1.1 76,390 - +0.3 76,390 - -0.5 76,390 - -3.2 76,390 - +0.1 76,390 - -0.6 76,390 - -1.8 76,390 - +0.0 76,559 + -0.4 76,560 - +0.0 76,560 - -0.2 76,560 - +0.1 76,560 - +0.4 77,087 + GFF3258 0.25 -0.2 77,087 + GFF3258 0.25 +0.0 77,087 + GFF3258 0.25 -0.9 77,087 + GFF3258 0.25 +0.0 77,087 + GFF3258 0.25 +1.0 77,087 + GFF3258 0.25 -0.3 77,087 + GFF3258 0.25 -0.1 77,088 - GFF3258 0.25 -0.2 77,088 - GFF3258 0.25 +0.6 77,088 - GFF3258 0.25 +0.7 77,088 - GFF3258 0.25 -0.7 77,088 - GFF3258 0.25 +0.9 77,088 - GFF3258 0.25 -1.6 77,088 - GFF3258 0.25 +0.8 77,088 - GFF3258 0.25 +0.0 77,088 - GFF3258 0.25 +0.3 77,351 + GFF3258 0.34 +1.1 77,351 + GFF3258 0.34 +0.3 77,351 + GFF3258 0.34 +1.6 77,352 - GFF3258 0.34 -0.5 77,352 - GFF3258 0.34 +0.2 77,352 - GFF3258 0.34 +1.5 77,417 + GFF3258 0.36 -0.5 77,417 + GFF3258 0.36 +0.8 77,417 + GFF3258 0.36 -0.2 77,417 + GFF3258 0.36 +0.3 77,417 + GFF3258 0.36 -0.1 77,417 + GFF3258 0.36 +0.8 77,417 + GFF3258 0.36 -1.0 77,417 + GFF3258 0.36 -0.7 77,417 + GFF3258 0.36 -0.2 77,417 + GFF3258 0.36 -0.6 77,417 + GFF3258 0.36 -0.5 77,417 + GFF3258 0.36 +0.1 77,417 + GFF3258 0.36 -0.5 77,417 + GFF3258 0.36 +0.3 77,417 + GFF3258 0.36 +0.4 77,417 + GFF3258 0.36 +0.6 77,417 + GFF3258 0.36 -0.4 77,418 - GFF3258 0.36 +0.8 77,418 - GFF3258 0.36 +1.2 77,418 - GFF3258 0.36 +0.1 77,418 - GFF3258 0.36 -1.1 77,418 - GFF3258 0.36 +1.8 77,418 - GFF3258 0.36 +0.4 77,418 - GFF3258 0.36 -0.6 77,418 - GFF3258 0.36 -0.2 77,418 - GFF3258 0.36 +0.4 77,418 - GFF3258 0.36 +0.0 77,418 - GFF3258 0.36 +0.8 77,418 - GFF3258 0.36 +0.0 77,418 - GFF3258 0.36 -0.4 77,418 - GFF3258 0.36 -0.2 77,418 - GFF3258 0.36 -0.2 77,418 - GFF3258 0.36 +0.5 77,418 - GFF3258 0.36 +0.5 77,418 - GFF3258 0.36 +0.5 77,418 - GFF3258 0.36 -0.2 77,418 - GFF3258 0.36 +0.1 77,418 - GFF3258 0.36 -1.3 77,418 - GFF3258 0.36 +0.3 77,418 - GFF3258 0.36 -0.2 77,628 - GFF3258 0.43 -0.3 77,765 + GFF3258 0.48 -0.5 78,137 + GFF3258 0.61 +0.3 78,138 - GFF3258 0.61 -0.0 78,138 - GFF3258 0.61 +0.1 78,423 + GFF3258 0.70 +0.3 78,423 + GFF3258 0.70 +2.3 78,423 + GFF3258 0.70 +0.4 78,423 + GFF3258 0.70 +0.5 78,423 + GFF3258 0.70 +0.8 78,423 + GFF3258 0.70 +0.5 78,423 + GFF3258 0.70 -0.6 78,423 + GFF3258 0.70 +0.4 78,424 - GFF3258 0.70 -0.9 78,424 - GFF3258 0.70 +0.3 78,424 - GFF3258 0.70 +0.7 78,424 - GFF3258 0.70 -0.1 78,424 - GFF3258 0.70 -1.0 78,712 + GFF3258 0.80 -1.6 78,712 + GFF3258 0.80 +0.1 78,712 + GFF3258 0.80 -0.2 78,713 - GFF3258 0.80 -0.2 78,713 - GFF3258 0.80 -0.1 78,990 + GFF3258 0.90 +3.2 78,991 - GFF3258 0.90 -0.2 79,348 + GFF3259 0.10 +0.2 79,348 + GFF3259 0.10 -0.0 79,348 + GFF3259 0.10 +0.5 79,348 + GFF3259 0.10 -0.5 79,349 - GFF3259 0.10 +2.4 79,451 - GFF3259 0.22 -0.2 79,510 + GFF3259 0.29 -0.5 79,510 + GFF3259 0.29 -0.2 79,511 - GFF3259 0.29 -0.2 79,511 - GFF3259 0.29 -1.9 79,570 + GFF3259 0.36 +0.4 79,570 + GFF3259 0.36 -0.2 79,603 + GFF3259 0.40 +0.5 79,603 + GFF3259 0.40 -0.9 79,603 + GFF3259 0.40 -0.1 79,603 + GFF3259 0.40 -0.3 79,603 + GFF3259 0.40 -1.1 79,603 + GFF3259 0.40 +0.1 79,603 + GFF3259 0.40 -0.5 79,604 - GFF3259 0.40 -0.5 79,604 - GFF3259 0.40 +0.2 79,604 - GFF3259 0.40 -0.2 79,604 - GFF3259 0.40 +0.7 79,972 + GFF3259 0.83 +0.6 79,973 - GFF3259 0.83 -0.9 80,209 - -1.5 80,226 + +0.3
Or see this region's nucleotide sequence