Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3256 and GFF3257 are separated by 101 nucleotides GFF3257 and GFF3258 are separated by 101 nucleotides
GFF3256 - Peptidase, M20/M25/M40 family, at 74,347 to 75,702
GFF3256
GFF3257 - hypothetical protein, at 75,804 to 76,262
GFF3257
GFF3258 - hypothetical protein, at 76,364 to 79,288
GFF3258
Position (kb) 75
76
77 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 75.026 kb on + strand, within GFF3256 at 75.026 kb on + strand, within GFF3256 at 75.027 kb on - strand, within GFF3256 at 75.027 kb on - strand, within GFF3256 at 75.048 kb on + strand, within GFF3256 at 75.049 kb on + strand, within GFF3256 at 75.049 kb on + strand, within GFF3256 at 75.298 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.357 kb on + strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.358 kb on - strand, within GFF3256 at 75.693 kb on + strand at 75.694 kb on - strand at 75.700 kb on + strand at 75.700 kb on + strand at 75.700 kb on + strand at 75.700 kb on + strand at 75.700 kb on + strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.701 kb on - strand at 75.747 kb on + strand at 75.747 kb on + strand at 75.747 kb on + strand at 75.748 kb on - strand at 75.748 kb on - strand at 75.748 kb on - strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.763 kb on + strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.764 kb on - strand at 75.838 kb on + strand at 75.838 kb on + strand at 75.838 kb on + strand at 75.838 kb on + strand at 75.838 kb on + strand at 75.839 kb on - strand at 75.839 kb on - strand at 76.355 kb on + strand at 76.355 kb on + strand at 76.355 kb on + strand at 76.356 kb on - strand at 76.356 kb on - strand at 76.356 kb on - strand at 76.356 kb on - strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.389 kb on + strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.390 kb on - strand at 76.559 kb on + strand at 76.560 kb on - strand at 76.560 kb on - strand at 76.560 kb on - strand at 76.560 kb on - strand at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.087 kb on + strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258 at 77.088 kb on - strand, within GFF3258
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 75,026 + GFF3256 0.50 -0.4 75,026 + GFF3256 0.50 +0.4 75,027 - GFF3256 0.50 -0.6 75,027 - GFF3256 0.50 -0.9 75,048 + GFF3256 0.52 -0.1 75,049 + GFF3256 0.52 -1.7 75,049 + GFF3256 0.52 +1.4 75,298 + GFF3256 0.70 -0.7 75,357 + GFF3256 0.74 +0.0 75,357 + GFF3256 0.74 -0.2 75,357 + GFF3256 0.74 -0.5 75,357 + GFF3256 0.74 +0.1 75,357 + GFF3256 0.74 -0.1 75,357 + GFF3256 0.74 -0.3 75,357 + GFF3256 0.74 -0.2 75,357 + GFF3256 0.74 +0.1 75,357 + GFF3256 0.74 -0.4 75,358 - GFF3256 0.75 +0.4 75,358 - GFF3256 0.75 +0.3 75,358 - GFF3256 0.75 +0.1 75,358 - GFF3256 0.75 +1.8 75,358 - GFF3256 0.75 +0.0 75,358 - GFF3256 0.75 -0.1 75,358 - GFF3256 0.75 +0.5 75,358 - GFF3256 0.75 +0.0 75,358 - GFF3256 0.75 +1.4 75,358 - GFF3256 0.75 +0.0 75,358 - GFF3256 0.75 +0.3 75,693 + -0.6 75,694 - -0.2 75,700 + -0.3 75,700 + -0.3 75,700 + -0.2 75,700 + -0.5 75,700 + -0.2 75,701 - -1.3 75,701 - +0.0 75,701 - -0.6 75,701 - +0.0 75,701 - -0.7 75,701 - -1.2 75,701 - +0.8 75,747 + -0.3 75,747 + -0.6 75,747 + -0.9 75,748 - -0.7 75,748 - +0.1 75,748 - -0.1 75,763 + +0.0 75,763 + -0.0 75,763 + +0.1 75,763 + +0.0 75,763 + +0.3 75,763 + -2.1 75,763 + -0.5 75,763 + -0.6 75,763 + -0.1 75,763 + +0.3 75,763 + +0.1 75,763 + -0.1 75,763 + -0.2 75,763 + -0.4 75,763 + -0.6 75,763 + -0.1 75,763 + +0.4 75,764 - -0.4 75,764 - -0.0 75,764 - -0.7 75,764 - -0.2 75,764 - -0.2 75,764 - -0.3 75,838 + +1.0 75,838 + -0.1 75,838 + +1.8 75,838 + -0.3 75,838 + -0.2 75,839 - +1.1 75,839 - +0.7 76,355 + -0.2 76,355 + +0.4 76,355 + +0.2 76,356 - +0.3 76,356 - -0.1 76,356 - -0.3 76,356 - +1.0 76,389 + -0.1 76,389 + -0.4 76,389 + +0.7 76,389 + +0.0 76,389 + +0.4 76,389 + -2.2 76,389 + +0.4 76,389 + -0.6 76,390 - -1.4 76,390 - +0.0 76,390 - -1.1 76,390 - +0.3 76,390 - -0.5 76,390 - -3.2 76,390 - +0.1 76,390 - -0.6 76,390 - -1.8 76,390 - +0.0 76,559 + -0.4 76,560 - +0.0 76,560 - -0.2 76,560 - +0.1 76,560 - +0.4 77,087 + GFF3258 0.25 -0.2 77,087 + GFF3258 0.25 +0.0 77,087 + GFF3258 0.25 -0.9 77,087 + GFF3258 0.25 +0.0 77,087 + GFF3258 0.25 +1.0 77,087 + GFF3258 0.25 -0.3 77,087 + GFF3258 0.25 -0.1 77,088 - GFF3258 0.25 -0.2 77,088 - GFF3258 0.25 +0.6 77,088 - GFF3258 0.25 +0.7 77,088 - GFF3258 0.25 -0.7 77,088 - GFF3258 0.25 +0.9 77,088 - GFF3258 0.25 -1.6 77,088 - GFF3258 0.25 +0.8 77,088 - GFF3258 0.25 +0.0 77,088 - GFF3258 0.25 +0.3
Or see this region's nucleotide sequence