Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3252 and GFF3253 are separated by 20 nucleotides GFF3253 and GFF3254 are separated by 236 nucleotides
GFF3252 - Periplasmic chaperone and peptidyl-prolyl cis-trans isomerase of outer membrane proteins SurA (EC 5.2.1.8), at 68,110 to 69,507
GFF3252
GFF3253 - LPS-assembly protein LptD @ Organic solvent tolerance protein precursor, at 69,528 to 72,122
GFF3253
GFF3254 - Phosphotransferase involved in threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 72,359 to 73,456
GFF3254
Position (kb)
69
70
71
72
73 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 69.535 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.536 kb on - strand at 69.536 kb on - strand at 69.536 kb on - strand at 69.583 kb on + strand at 69.584 kb on - strand at 72.345 kb on - strand at 72.345 kb on - strand at 72.345 kb on - strand at 72.355 kb on - strand at 72.357 kb on - strand at 72.533 kb on + strand, within GFF3254 at 72.533 kb on + strand, within GFF3254 at 72.534 kb on - strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.842 kb on + strand, within GFF3254 at 72.842 kb on + strand, within GFF3254 at 72.842 kb on + strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.079 kb on + strand, within GFF3254 at 73.079 kb on + strand, within GFF3254 at 73.079 kb on + strand, within GFF3254 at 73.080 kb on - strand, within GFF3254 at 73.080 kb on - strand, within GFF3254 at 73.080 kb on - strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 69,535 + +1.0 69,535 + +0.0 69,535 + +0.1 69,535 + -0.5 69,535 + +0.3 69,535 + +0.0 69,536 - -0.3 69,536 - -0.3 69,536 - +1.5 69,583 + +0.5 69,584 - +0.5 72,345 - -0.2 72,345 - +1.5 72,345 - -0.9 72,355 - -0.3 72,357 - -1.4 72,533 + GFF3254 0.16 -1.9 72,533 + GFF3254 0.16 +0.3 72,534 - GFF3254 0.16 -0.6 72,776 + GFF3254 0.38 +0.2 72,776 + GFF3254 0.38 +0.6 72,776 + GFF3254 0.38 +0.8 72,776 + GFF3254 0.38 +0.1 72,776 + GFF3254 0.38 +0.7 72,777 - GFF3254 0.38 -0.8 72,777 - GFF3254 0.38 -0.2 72,777 - GFF3254 0.38 -1.6 72,777 - GFF3254 0.38 -0.2 72,777 - GFF3254 0.38 -1.7 72,842 + GFF3254 0.44 -1.7 72,842 + GFF3254 0.44 +0.0 72,842 + GFF3254 0.44 -1.3 72,843 - GFF3254 0.44 +1.1 72,843 - GFF3254 0.44 -0.3 72,843 - GFF3254 0.44 +0.1 72,843 - GFF3254 0.44 +0.3 72,843 - GFF3254 0.44 +0.2 72,890 + GFF3254 0.48 -0.9 72,890 + GFF3254 0.48 -0.9 72,890 + GFF3254 0.48 +1.0 72,890 + GFF3254 0.48 +1.0 72,890 + GFF3254 0.48 -1.5 72,890 + GFF3254 0.48 +0.6 72,890 + GFF3254 0.48 -0.5 72,890 + GFF3254 0.48 -1.0 72,890 + GFF3254 0.48 -0.3 72,890 + GFF3254 0.48 -0.1 72,890 + GFF3254 0.48 +0.4 72,890 + GFF3254 0.48 -0.6 72,890 + GFF3254 0.48 +0.0 72,890 + GFF3254 0.48 +0.3 72,891 - GFF3254 0.48 -0.2 72,891 - GFF3254 0.48 -1.1 72,891 - GFF3254 0.48 +0.1 72,891 - GFF3254 0.48 -1.1 72,891 - GFF3254 0.48 -0.5 72,891 - GFF3254 0.48 -0.6 72,891 - GFF3254 0.48 -0.4 72,891 - GFF3254 0.48 +0.3 72,891 - GFF3254 0.48 +0.3 72,891 - GFF3254 0.48 -1.2 72,891 - GFF3254 0.48 -1.2 72,891 - GFF3254 0.48 +0.3 72,995 + GFF3254 0.58 -0.5 72,995 + GFF3254 0.58 +2.8 72,995 + GFF3254 0.58 -1.6 72,995 + GFF3254 0.58 -0.6 72,995 + GFF3254 0.58 -1.2 72,995 + GFF3254 0.58 +1.0 72,995 + GFF3254 0.58 -1.2 72,995 + GFF3254 0.58 -0.7 72,995 + GFF3254 0.58 -0.5 72,995 + GFF3254 0.58 -1.0 72,996 - GFF3254 0.58 -0.4 72,996 - GFF3254 0.58 -1.5 72,996 - GFF3254 0.58 -2.1 72,996 - GFF3254 0.58 +0.5 72,996 - GFF3254 0.58 -0.3 72,996 - GFF3254 0.58 -2.4 73,026 + GFF3254 0.61 -1.1 73,026 + GFF3254 0.61 -0.8 73,026 + GFF3254 0.61 -1.2 73,026 + GFF3254 0.61 -1.3 73,026 + GFF3254 0.61 -0.8 73,026 + GFF3254 0.61 -1.7 73,026 + GFF3254 0.61 -0.4 73,027 - GFF3254 0.61 -0.3 73,027 - GFF3254 0.61 -1.0 73,027 - GFF3254 0.61 -0.7 73,027 - GFF3254 0.61 -0.3 73,027 - GFF3254 0.61 +1.2 73,027 - GFF3254 0.61 -0.4 73,027 - GFF3254 0.61 +0.5 73,079 + GFF3254 0.66 -0.2 73,079 + GFF3254 0.66 -0.3 73,079 + GFF3254 0.66 +1.3 73,080 - GFF3254 0.66 -0.1 73,080 - GFF3254 0.66 -0.3 73,080 - GFF3254 0.66 -1.1 73,094 + GFF3254 0.67 -0.9 73,094 + GFF3254 0.67 -1.0 73,094 + GFF3254 0.67 -0.3 73,094 + GFF3254 0.67 +0.3 73,094 + GFF3254 0.67 -1.1 73,094 + GFF3254 0.67 -0.2 73,094 + GFF3254 0.67 -0.1 73,094 + GFF3254 0.67 +0.5 73,094 + GFF3254 0.67 -0.3 73,094 + GFF3254 0.67 -2.0 73,095 - GFF3254 0.67 -0.7 73,095 - GFF3254 0.67 -1.1 73,095 - GFF3254 0.67 -1.1 73,095 - GFF3254 0.67 -1.4 73,095 - GFF3254 0.67 -0.5 73,095 - GFF3254 0.67 -0.2 73,095 - GFF3254 0.67 -0.2 73,095 - GFF3254 0.67 -0.7 73,095 - GFF3254 0.67 -0.2 73,095 - GFF3254 0.67 +2.2 73,095 - GFF3254 0.67 -1.2
Or see this region's nucleotide sequence