Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3251 and GFF3252 are separated by 4 nucleotides GFF3252 and GFF3253 are separated by 20 nucleotides
GFF3251 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182), at 67,338 to 68,105
GFF3251
GFF3252 - Periplasmic chaperone and peptidyl-prolyl cis-trans isomerase of outer membrane proteins SurA (EC 5.2.1.8), at 68,110 to 69,507
GFF3252
GFF3253 - LPS-assembly protein LptD @ Organic solvent tolerance protein precursor, at 69,528 to 72,122
GFF3253
Position (kb) 68
69
70 Strain fitness (log2 ratio) -2
-1
0
1
2 at 67.248 kb on + strand at 67.266 kb on + strand at 67.295 kb on + strand at 67.295 kb on + strand at 67.295 kb on + strand at 67.296 kb on - strand at 67.296 kb on - strand at 67.372 kb on + strand at 67.372 kb on + strand at 67.372 kb on + strand at 67.372 kb on + strand at 67.372 kb on + strand at 67.372 kb on + strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.373 kb on - strand at 67.440 kb on + strand, within GFF3251 at 67.441 kb on - strand, within GFF3251 at 67.441 kb on - strand, within GFF3251 at 67.441 kb on - strand, within GFF3251 at 67.621 kb on + strand, within GFF3251 at 67.631 kb on - strand, within GFF3251 at 67.631 kb on - strand, within GFF3251 at 67.631 kb on - strand, within GFF3251 at 67.663 kb on + strand, within GFF3251 at 67.663 kb on + strand, within GFF3251 at 67.663 kb on + strand, within GFF3251 at 67.663 kb on + strand, within GFF3251 at 67.663 kb on + strand, within GFF3251 at 67.664 kb on - strand, within GFF3251 at 67.664 kb on - strand, within GFF3251 at 67.664 kb on - strand, within GFF3251 at 67.664 kb on - strand, within GFF3251 at 67.664 kb on - strand, within GFF3251 at 67.664 kb on - strand, within GFF3251 at 67.664 kb on - strand, within GFF3251 at 67.664 kb on - strand, within GFF3251 at 67.674 kb on - strand, within GFF3251 at 67.747 kb on + strand, within GFF3251 at 67.747 kb on + strand, within GFF3251 at 67.747 kb on + strand, within GFF3251 at 67.747 kb on + strand, within GFF3251 at 67.747 kb on + strand, within GFF3251 at 67.747 kb on + strand, within GFF3251 at 67.747 kb on + strand, within GFF3251 at 67.747 kb on + strand, within GFF3251 at 67.748 kb on - strand, within GFF3251 at 67.748 kb on - strand, within GFF3251 at 67.748 kb on - strand, within GFF3251 at 67.748 kb on - strand, within GFF3251 at 67.748 kb on - strand, within GFF3251 at 67.748 kb on - strand, within GFF3251 at 67.786 kb on + strand, within GFF3251 at 67.786 kb on + strand, within GFF3251 at 67.786 kb on + strand, within GFF3251 at 67.786 kb on + strand, within GFF3251 at 67.786 kb on + strand, within GFF3251 at 67.786 kb on + strand, within GFF3251 at 67.786 kb on + strand, within GFF3251 at 67.786 kb on + strand, within GFF3251 at 67.787 kb on - strand, within GFF3251 at 67.787 kb on - strand, within GFF3251 at 67.787 kb on - strand, within GFF3251 at 67.787 kb on - strand, within GFF3251 at 67.787 kb on - strand, within GFF3251 at 67.787 kb on - strand, within GFF3251 at 68.132 kb on + strand at 68.132 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.535 kb on + strand at 69.536 kb on - strand at 69.536 kb on - strand at 69.536 kb on - strand at 69.583 kb on + strand at 69.584 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 67,248 + -0.6 67,266 + +0.3 67,295 + +0.2 67,295 + -0.5 67,295 + -0.7 67,296 - -0.5 67,296 - -2.0 67,372 + +0.3 67,372 + -1.4 67,372 + +0.1 67,372 + -0.2 67,372 + +0.0 67,372 + +0.4 67,373 - +0.1 67,373 - +0.3 67,373 - +0.7 67,373 - +0.5 67,373 - +0.6 67,373 - +1.8 67,373 - -0.1 67,373 - -0.3 67,373 - +0.4 67,373 - -0.9 67,440 + GFF3251 0.13 -2.1 67,441 - GFF3251 0.13 -1.2 67,441 - GFF3251 0.13 +0.5 67,441 - GFF3251 0.13 -0.1 67,621 + GFF3251 0.37 +0.7 67,631 - GFF3251 0.38 -0.4 67,631 - GFF3251 0.38 -0.5 67,631 - GFF3251 0.38 -0.2 67,663 + GFF3251 0.42 +0.1 67,663 + GFF3251 0.42 -1.0 67,663 + GFF3251 0.42 -1.7 67,663 + GFF3251 0.42 -1.6 67,663 + GFF3251 0.42 -0.7 67,664 - GFF3251 0.42 -0.2 67,664 - GFF3251 0.42 -1.7 67,664 - GFF3251 0.42 -0.5 67,664 - GFF3251 0.42 +0.3 67,664 - GFF3251 0.42 -0.2 67,664 - GFF3251 0.42 -1.5 67,664 - GFF3251 0.42 -1.3 67,664 - GFF3251 0.42 -0.7 67,674 - GFF3251 0.44 -1.5 67,747 + GFF3251 0.53 +0.3 67,747 + GFF3251 0.53 -0.3 67,747 + GFF3251 0.53 -0.7 67,747 + GFF3251 0.53 -0.6 67,747 + GFF3251 0.53 -0.6 67,747 + GFF3251 0.53 +0.5 67,747 + GFF3251 0.53 +0.3 67,747 + GFF3251 0.53 -0.5 67,748 - GFF3251 0.53 -0.7 67,748 - GFF3251 0.53 +1.1 67,748 - GFF3251 0.53 +0.0 67,748 - GFF3251 0.53 -0.6 67,748 - GFF3251 0.53 -1.2 67,748 - GFF3251 0.53 -1.0 67,786 + GFF3251 0.58 -1.2 67,786 + GFF3251 0.58 +0.8 67,786 + GFF3251 0.58 +0.7 67,786 + GFF3251 0.58 -0.2 67,786 + GFF3251 0.58 +0.5 67,786 + GFF3251 0.58 -0.0 67,786 + GFF3251 0.58 +0.2 67,786 + GFF3251 0.58 +0.6 67,787 - GFF3251 0.58 -0.9 67,787 - GFF3251 0.58 -0.2 67,787 - GFF3251 0.58 -0.4 67,787 - GFF3251 0.58 +0.3 67,787 - GFF3251 0.58 -0.2 67,787 - GFF3251 0.58 +0.2 68,132 + -0.7 68,132 + +0.4 69,535 + +1.0 69,535 + +0.0 69,535 + +0.1 69,535 + -0.5 69,535 + +0.3 69,535 + +0.0 69,536 - -0.3 69,536 - -0.3 69,536 - +1.5 69,583 + +0.5 69,584 - +0.5
Or see this region's nucleotide sequence