Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3231 and GFF3232 overlap by 4 nucleotides GFF3232 and GFF3233 are separated by 116 nucleotides GFF3233 and GFF3234 are separated by 1 nucleotides GFF3234 and GFF3235 are separated by 55 nucleotides GFF3235 and GFF3236 overlap by 1 nucleotides GFF3236 and GFF3237 overlap by 4 nucleotides
GFF3231 - hypothetical protein, at 50,575 to 51,186
GFF3231
GFF3232 - Phage lysozyme R (EC 3.2.1.17), at 51,183 to 51,758
GFF3232
GFF3233 - hypothetical protein, at 51,875 to 52,627
GFF3233
GFF3234 - no description, at 52,629 to 52,748
GFF3234
GFF3235 - no description, at 52,804 to 53,175
GFF3235
GFF3236 - no description, at 53,175 to 53,573
GFF3236
GFF3237 - no description, at 53,570 to 53,809
GFF3237
Position (kb)
51
52
53 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 50.948 kb on + strand, within GFF3231 at 50.948 kb on + strand, within GFF3231 at 50.948 kb on + strand, within GFF3231 at 50.948 kb on + strand, within GFF3231 at 50.949 kb on + strand, within GFF3231 at 50.949 kb on - strand, within GFF3231 at 50.949 kb on - strand, within GFF3231 at 50.949 kb on - strand, within GFF3231 at 51.011 kb on + strand, within GFF3231 at 51.011 kb on + strand, within GFF3231 at 51.289 kb on + strand, within GFF3232 at 51.289 kb on + strand, within GFF3232 at 51.290 kb on - strand, within GFF3232 at 51.325 kb on + strand, within GFF3232 at 51.326 kb on - strand, within GFF3232 at 51.409 kb on + strand, within GFF3232 at 51.409 kb on + strand, within GFF3232 at 51.409 kb on + strand, within GFF3232 at 51.410 kb on - strand, within GFF3232 at 51.410 kb on - strand, within GFF3232 at 51.410 kb on - strand, within GFF3232 at 51.410 kb on - strand, within GFF3232 at 51.424 kb on + strand, within GFF3232 at 51.424 kb on + strand, within GFF3232 at 51.424 kb on + strand, within GFF3232 at 51.424 kb on + strand, within GFF3232 at 51.425 kb on - strand, within GFF3232 at 51.433 kb on + strand, within GFF3232 at 51.433 kb on + strand, within GFF3232 at 51.483 kb on + strand, within GFF3232 at 51.484 kb on + strand, within GFF3232 at 51.484 kb on + strand, within GFF3232 at 51.484 kb on + strand, within GFF3232 at 51.484 kb on + strand, within GFF3232 at 51.485 kb on - strand, within GFF3232 at 52.789 kb on - strand at 52.821 kb on - strand at 52.949 kb on - strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.203 kb on + strand at 53.203 kb on + strand at 53.204 kb on - strand at 53.204 kb on - strand at 53.514 kb on + strand, within GFF3236 at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 50,948 + GFF3231 0.61 -0.2 50,948 + GFF3231 0.61 -0.7 50,948 + GFF3231 0.61 -0.1 50,948 + GFF3231 0.61 -0.2 50,949 + GFF3231 0.61 +0.2 50,949 - GFF3231 0.61 +0.8 50,949 - GFF3231 0.61 -1.1 50,949 - GFF3231 0.61 +0.3 51,011 + GFF3231 0.71 -0.2 51,011 + GFF3231 0.71 -0.3 51,289 + GFF3232 0.18 -0.5 51,289 + GFF3232 0.18 -1.0 51,290 - GFF3232 0.19 +0.1 51,325 + GFF3232 0.25 +0.3 51,326 - GFF3232 0.25 -0.2 51,409 + GFF3232 0.39 -1.5 51,409 + GFF3232 0.39 -0.1 51,409 + GFF3232 0.39 +0.0 51,410 - GFF3232 0.39 -0.7 51,410 - GFF3232 0.39 +0.6 51,410 - GFF3232 0.39 -0.8 51,410 - GFF3232 0.39 -0.3 51,424 + GFF3232 0.42 +0.3 51,424 + GFF3232 0.42 +0.3 51,424 + GFF3232 0.42 +0.7 51,424 + GFF3232 0.42 +0.0 51,425 - GFF3232 0.42 -0.4 51,433 + GFF3232 0.43 +1.1 51,433 + GFF3232 0.43 +0.3 51,483 + GFF3232 0.52 -0.6 51,484 + GFF3232 0.52 -1.0 51,484 + GFF3232 0.52 -0.7 51,484 + GFF3232 0.52 +2.2 51,484 + GFF3232 0.52 -1.2 51,485 - GFF3232 0.52 -0.2 52,789 - +0.7 52,821 - -0.5 52,949 - GFF3235 0.39 -0.7 53,115 + GFF3235 0.84 +0.0 53,115 + GFF3235 0.84 +0.0 53,115 + GFF3235 0.84 +0.7 53,115 + GFF3235 0.84 -0.4 53,115 + GFF3235 0.84 -0.1 53,115 + GFF3235 0.84 -0.2 53,116 - GFF3235 0.84 -0.8 53,116 - GFF3235 0.84 +0.1 53,116 - GFF3235 0.84 +0.6 53,116 - GFF3235 0.84 -0.5 53,116 - GFF3235 0.84 -0.5 53,116 - GFF3235 0.84 +0.1 53,116 - GFF3235 0.84 +0.5 53,203 + -0.5 53,203 + -1.6 53,204 - +0.8 53,204 - +0.2 53,514 + GFF3236 0.85 +0.1 53,591 + -0.2 53,591 + -1.0 53,591 + +0.8 53,591 + -0.5 53,591 + +0.8 53,592 - -0.5 53,592 - -0.3 53,592 - -2.1 53,592 - -0.5 53,592 - -0.1
Or see this region's nucleotide sequence