Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3171 and GFF3172 are separated by 202 nucleotides GFF3172 and GFF3173 are separated by 32 nucleotides GFF3173 and GFF3174 are separated by 106 nucleotides
GFF3171 - Uncharacterized protein conserved in bacteria, at 611,559 to 612,785
GFF3171
GFF3172 - Glycosidases, at 612,988 to 613,320
GFF3172
GFF3173 - Transcriptional regulator, IclR family, at 613,353 to 614,159
GFF3173
GFF3174 - Oxidoreductase, GMC family, at 614,266 to 615,906
GFF3174
Position (kb) 612
613
614 Strain fitness (log2 ratio) -2
-1
0
1 at 611.992 kb on + strand, within GFF3171 at 611.992 kb on + strand, within GFF3171 at 612.038 kb on - strand, within GFF3171 at 612.038 kb on - strand, within GFF3171 at 612.038 kb on - strand, within GFF3171 at 612.079 kb on + strand, within GFF3171 at 612.169 kb on + strand, within GFF3171 at 612.170 kb on - strand, within GFF3171 at 612.394 kb on + strand, within GFF3171 at 612.394 kb on + strand, within GFF3171 at 612.394 kb on + strand, within GFF3171 at 612.395 kb on - strand, within GFF3171 at 612.395 kb on - strand, within GFF3171 at 612.544 kb on + strand, within GFF3171 at 612.544 kb on + strand, within GFF3171 at 612.568 kb on + strand, within GFF3171 at 612.569 kb on - strand, within GFF3171 at 612.569 kb on - strand, within GFF3171 at 612.569 kb on - strand, within GFF3171 at 612.658 kb on + strand, within GFF3171 at 612.658 kb on + strand, within GFF3171 at 612.659 kb on - strand, within GFF3171 at 612.715 kb on + strand at 612.715 kb on + strand at 612.755 kb on - strand at 612.755 kb on - strand at 612.755 kb on - strand at 612.755 kb on - strand at 612.987 kb on + strand at 612.987 kb on + strand at 612.987 kb on + strand at 612.987 kb on + strand at 612.987 kb on + strand at 612.987 kb on + strand at 612.988 kb on - strand at 612.988 kb on - strand at 612.988 kb on - strand at 612.988 kb on - strand at 612.988 kb on - strand at 612.988 kb on - strand at 612.988 kb on - strand at 613.318 kb on + strand at 613.318 kb on + strand at 613.318 kb on + strand at 613.318 kb on + strand at 613.318 kb on + strand at 613.318 kb on + strand at 613.319 kb on - strand at 613.319 kb on - strand at 613.319 kb on - strand at 613.319 kb on - strand at 613.541 kb on - strand, within GFF3173 at 613.765 kb on + strand, within GFF3173 at 613.765 kb on + strand, within GFF3173 at 613.765 kb on + strand, within GFF3173 at 613.766 kb on - strand, within GFF3173 at 614.287 kb on + strand at 614.287 kb on + strand at 614.287 kb on + strand at 614.287 kb on + strand at 614.288 kb on - strand at 614.288 kb on - strand at 614.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 611,992 + GFF3171 0.35 +0.5 611,992 + GFF3171 0.35 +0.5 612,038 - GFF3171 0.39 +0.7 612,038 - GFF3171 0.39 -0.5 612,038 - GFF3171 0.39 -0.8 612,079 + GFF3171 0.42 -1.3 612,169 + GFF3171 0.50 -0.6 612,170 - GFF3171 0.50 -0.0 612,394 + GFF3171 0.68 -0.2 612,394 + GFF3171 0.68 +0.2 612,394 + GFF3171 0.68 -0.2 612,395 - GFF3171 0.68 -0.7 612,395 - GFF3171 0.68 -0.9 612,544 + GFF3171 0.80 +0.3 612,544 + GFF3171 0.80 -0.5 612,568 + GFF3171 0.82 +1.1 612,569 - GFF3171 0.82 -0.2 612,569 - GFF3171 0.82 -1.9 612,569 - GFF3171 0.82 +0.4 612,658 + GFF3171 0.90 -1.7 612,658 + GFF3171 0.90 -0.4 612,659 - GFF3171 0.90 +1.0 612,715 + -0.6 612,715 + +1.5 612,755 - -0.2 612,755 - -0.5 612,755 - -0.5 612,755 - -0.1 612,987 + +0.1 612,987 + -0.6 612,987 + +0.8 612,987 + -1.4 612,987 + -1.3 612,987 + -0.0 612,988 - -0.1 612,988 - -1.3 612,988 - +1.1 612,988 - -1.5 612,988 - -0.2 612,988 - -0.2 612,988 - -0.2 613,318 + -0.3 613,318 + +0.0 613,318 + -0.2 613,318 + +0.5 613,318 + -0.1 613,318 + -0.0 613,319 - -0.3 613,319 - +0.0 613,319 - -1.2 613,319 - +0.1 613,541 - GFF3173 0.23 -1.4 613,765 + GFF3173 0.51 +0.9 613,765 + GFF3173 0.51 -0.8 613,765 + GFF3173 0.51 +0.5 613,766 - GFF3173 0.51 -0.2 614,287 + +1.1 614,287 + -0.5 614,287 + -0.9 614,287 + -0.7 614,288 - -1.4 614,288 - -0.6 614,288 - +0.1
Or see this region's nucleotide sequence