Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3116 and GFF3117 are separated by 5 nucleotides GFF3117 and GFF3118 overlap by 14 nucleotides
GFF3116 - Peptide chain release factor 2 @ programmed frameshift-containing, at 542,902 to 543,804
GFF3116
GFF3117 - Beta-phosphoglucomutase (EC 5.4.2.6), at 543,810 to 544,508
GFF3117
GFF3118 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 544,495 to 547,188
GFF3118
Position (kb) 543
544
545 Strain fitness (log2 ratio) -1
0
1
2 at 543.802 kb on + strand at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.102 kb on + strand, within GFF3117 at 544.103 kb on - strand, within GFF3117 at 544.455 kb on + strand at 544.546 kb on + strand at 544.547 kb on - strand at 544.562 kb on - strand at 544.897 kb on + strand, within GFF3118 at 544.898 kb on - strand, within GFF3118 at 544.930 kb on + strand, within GFF3118 at 544.930 kb on + strand, within GFF3118 at 545.062 kb on + strand, within GFF3118 at 545.062 kb on + strand, within GFF3118 at 545.063 kb on - strand, within GFF3118 at 545.149 kb on + strand, within GFF3118 at 545.149 kb on + strand, within GFF3118 at 545.150 kb on - strand, within GFF3118 at 545.150 kb on - strand, within GFF3118 at 545.332 kb on + strand, within GFF3118 at 545.332 kb on + strand, within GFF3118 at 545.332 kb on + strand, within GFF3118 at 545.332 kb on + strand, within GFF3118 at 545.332 kb on + strand, within GFF3118 at 545.333 kb on - strand, within GFF3118 at 545.333 kb on - strand, within GFF3118 at 545.333 kb on - strand, within GFF3118 at 545.371 kb on + strand, within GFF3118 at 545.404 kb on + strand, within GFF3118 at 545.404 kb on + strand, within GFF3118 at 545.404 kb on + strand, within GFF3118
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 543,802 + +0.0 544,068 + GFF3117 0.37 -0.9 544,068 + GFF3117 0.37 -0.8 544,068 + GFF3117 0.37 +0.1 544,068 + GFF3117 0.37 -0.9 544,068 + GFF3117 0.37 +0.1 544,068 + GFF3117 0.37 -1.0 544,069 - GFF3117 0.37 -0.2 544,069 - GFF3117 0.37 -0.8 544,069 - GFF3117 0.37 -0.4 544,069 - GFF3117 0.37 -0.6 544,069 - GFF3117 0.37 -0.2 544,102 + GFF3117 0.42 +2.1 544,103 - GFF3117 0.42 -1.0 544,455 + +0.1 544,546 + -0.1 544,547 - -0.0 544,562 - +1.1 544,897 + GFF3118 0.15 -1.5 544,898 - GFF3118 0.15 +1.0 544,930 + GFF3118 0.16 +1.1 544,930 + GFF3118 0.16 -0.5 545,062 + GFF3118 0.21 -0.1 545,062 + GFF3118 0.21 -1.0 545,063 - GFF3118 0.21 +0.2 545,149 + GFF3118 0.24 -0.8 545,149 + GFF3118 0.24 -0.5 545,150 - GFF3118 0.24 -1.0 545,150 - GFF3118 0.24 +0.0 545,332 + GFF3118 0.31 -0.9 545,332 + GFF3118 0.31 +0.8 545,332 + GFF3118 0.31 -1.5 545,332 + GFF3118 0.31 +0.9 545,332 + GFF3118 0.31 -1.3 545,333 - GFF3118 0.31 -1.0 545,333 - GFF3118 0.31 -0.8 545,333 - GFF3118 0.31 -0.9 545,371 + GFF3118 0.33 -0.9 545,404 + GFF3118 0.34 +1.4 545,404 + GFF3118 0.34 +0.5 545,404 + GFF3118 0.34 -0.8
Or see this region's nucleotide sequence