Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3115 and GFF3116 are separated by 388 nucleotides GFF3116 and GFF3117 are separated by 5 nucleotides GFF3117 and GFF3118 overlap by 14 nucleotides
GFF3115 - Methionine ABC transporter substrate-binding protein, at 541,698 to 542,513
GFF3115
GFF3116 - Peptide chain release factor 2 @ programmed frameshift-containing, at 542,902 to 543,804
GFF3116
GFF3117 - Beta-phosphoglucomutase (EC 5.4.2.6), at 543,810 to 544,508
GFF3117
GFF3118 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 544,495 to 547,188
GFF3118
Position (kb) 542
543
544 Strain fitness (log2 ratio) -2
-1
0
1
2 at 541.902 kb on + strand, within GFF3115 at 541.902 kb on + strand, within GFF3115 at 541.902 kb on + strand, within GFF3115 at 541.903 kb on - strand, within GFF3115 at 541.903 kb on - strand, within GFF3115 at 542.046 kb on + strand, within GFF3115 at 542.046 kb on + strand, within GFF3115 at 542.046 kb on + strand, within GFF3115 at 542.046 kb on + strand, within GFF3115 at 542.047 kb on - strand, within GFF3115 at 542.047 kb on - strand, within GFF3115 at 542.047 kb on - strand, within GFF3115 at 542.047 kb on - strand, within GFF3115 at 542.376 kb on + strand, within GFF3115 at 542.625 kb on + strand at 543.802 kb on + strand at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.068 kb on + strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.069 kb on - strand, within GFF3117 at 544.102 kb on + strand, within GFF3117 at 544.103 kb on - strand, within GFF3117 at 544.455 kb on + strand at 544.546 kb on + strand at 544.547 kb on - strand at 544.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 541,902 + GFF3115 0.25 -0.3 541,902 + GFF3115 0.25 -2.0 541,902 + GFF3115 0.25 +2.3 541,903 - GFF3115 0.25 +1.2 541,903 - GFF3115 0.25 -1.6 542,046 + GFF3115 0.43 -0.3 542,046 + GFF3115 0.43 +1.5 542,046 + GFF3115 0.43 -0.0 542,046 + GFF3115 0.43 +0.0 542,047 - GFF3115 0.43 +0.5 542,047 - GFF3115 0.43 -0.0 542,047 - GFF3115 0.43 +0.1 542,047 - GFF3115 0.43 +1.1 542,376 + GFF3115 0.83 -1.0 542,625 + -0.0 543,802 + +0.0 544,068 + GFF3117 0.37 -0.9 544,068 + GFF3117 0.37 -0.8 544,068 + GFF3117 0.37 +0.1 544,068 + GFF3117 0.37 -0.9 544,068 + GFF3117 0.37 +0.1 544,068 + GFF3117 0.37 -1.0 544,069 - GFF3117 0.37 -0.2 544,069 - GFF3117 0.37 -0.8 544,069 - GFF3117 0.37 -0.4 544,069 - GFF3117 0.37 -0.6 544,069 - GFF3117 0.37 -0.2 544,102 + GFF3117 0.42 +2.1 544,103 - GFF3117 0.42 -1.0 544,455 + +0.1 544,546 + -0.1 544,547 - -0.0 544,562 - +1.1
Or see this region's nucleotide sequence