Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF309 and GFF310 overlap by 4 nucleotides GFF310 and GFF311 overlap by 4 nucleotides GFF311 and GFF312 overlap by 1 nucleotides
GFF309 - 3-hydroxydecanoyl-[ACP] dehydratase (EC 4.2.1.59), at 342,059 to 342,532
GFF309
GFF310 - FIG025233: SAM-dependent methyltransferases, at 342,529 to 343,305
GFF310
GFF311 - Polysaccharide deacetylase, at 343,302 to 344,144
GFF311
GFF312 - FIG021862: membrane protein, exporter, at 344,144 to 346,486
GFF312
Position (kb) 343
344
345 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 342.342 kb on + strand, within GFF309 at 342.342 kb on + strand, within GFF309 at 342.343 kb on - strand, within GFF309 at 342.851 kb on + strand, within GFF310 at 342.851 kb on + strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 342.852 kb on - strand, within GFF310 at 343.001 kb on + strand, within GFF310 at 343.229 kb on + strand at 343.229 kb on + strand at 343.229 kb on + strand at 343.230 kb on - strand at 343.230 kb on - strand at 343.377 kb on + strand at 343.377 kb on + strand at 343.377 kb on + strand at 343.377 kb on + strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 343.378 kb on - strand at 344.376 kb on + strand at 344.376 kb on + strand at 344.377 kb on - strand at 344.377 kb on - strand at 344.526 kb on + strand, within GFF312 at 344.526 kb on + strand, within GFF312 at 344.526 kb on + strand, within GFF312 at 344.526 kb on + strand, within GFF312 at 344.527 kb on - strand, within GFF312 at 344.527 kb on - strand, within GFF312 at 344.593 kb on - strand, within GFF312 at 344.593 kb on - strand, within GFF312 at 344.593 kb on - strand, within GFF312 at 344.601 kb on + strand, within GFF312 at 344.602 kb on - strand, within GFF312 at 344.742 kb on + strand, within GFF312 at 344.742 kb on + strand, within GFF312 at 344.743 kb on - strand, within GFF312 at 344.743 kb on - strand, within GFF312 at 344.743 kb on - strand, within GFF312 at 344.743 kb on - strand, within GFF312 at 344.743 kb on - strand, within GFF312 at 344.743 kb on - strand, within GFF312 at 344.743 kb on - strand, within GFF312 at 344.752 kb on + strand, within GFF312 at 344.752 kb on + strand, within GFF312 at 344.753 kb on - strand, within GFF312 at 344.753 kb on - strand, within GFF312 at 344.753 kb on - strand, within GFF312 at 344.834 kb on + strand, within GFF312 at 344.835 kb on - strand, within GFF312 at 344.979 kb on + strand, within GFF312 at 344.980 kb on - strand, within GFF312 at 345.070 kb on - strand, within GFF312 at 345.070 kb on - strand, within GFF312
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 342,342 + GFF309 0.60 +0.5 342,342 + GFF309 0.60 -0.7 342,343 - GFF309 0.60 +0.3 342,851 + GFF310 0.41 +0.2 342,851 + GFF310 0.41 +0.2 342,852 - GFF310 0.42 +0.7 342,852 - GFF310 0.42 -0.1 342,852 - GFF310 0.42 +1.2 343,001 + GFF310 0.61 +1.7 343,229 + +0.5 343,229 + -1.4 343,229 + -1.0 343,230 - -0.9 343,230 - +0.8 343,377 + -0.9 343,377 + +0.2 343,377 + -0.1 343,377 + +0.1 343,378 - +0.1 343,378 - -0.6 343,378 - -0.3 343,378 - -0.1 343,378 - +1.6 343,378 - -0.4 343,378 - +0.2 343,378 - +0.2 344,376 + +0.7 344,376 + -1.9 344,377 - -0.4 344,377 - +1.2 344,526 + GFF312 0.16 +0.9 344,526 + GFF312 0.16 -0.8 344,526 + GFF312 0.16 +1.2 344,526 + GFF312 0.16 -0.8 344,527 - GFF312 0.16 +1.1 344,527 - GFF312 0.16 +0.5 344,593 - GFF312 0.19 -0.5 344,593 - GFF312 0.19 -0.7 344,593 - GFF312 0.19 -0.7 344,601 + GFF312 0.20 -0.3 344,602 - GFF312 0.20 -0.1 344,742 + GFF312 0.26 +1.8 344,742 + GFF312 0.26 -0.5 344,743 - GFF312 0.26 -0.2 344,743 - GFF312 0.26 +0.0 344,743 - GFF312 0.26 -1.4 344,743 - GFF312 0.26 -0.7 344,743 - GFF312 0.26 -0.9 344,743 - GFF312 0.26 +0.6 344,743 - GFF312 0.26 -0.2 344,752 + GFF312 0.26 -0.5 344,752 + GFF312 0.26 +0.0 344,753 - GFF312 0.26 -0.7 344,753 - GFF312 0.26 -0.4 344,753 - GFF312 0.26 +0.5 344,834 + GFF312 0.29 +0.4 344,835 - GFF312 0.29 -0.8 344,979 + GFF312 0.36 -0.7 344,980 - GFF312 0.36 -1.4 345,070 - GFF312 0.40 +3.0 345,070 - GFF312 0.40 -0.1
Or see this region's nucleotide sequence