Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3045 and GFF3046 are separated by 64 nucleotides GFF3046 and GFF3047 overlap by 4 nucleotides GFF3047 and GFF3048 are separated by 21 nucleotides
GFF3045 - Glutamine ABC transporter, substrate-binding protein GlnH, at 480,919 to 481,761
GFF3045
GFF3046 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 481,826 to 482,617
GFF3046
GFF3047 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 482,614 to 483,393
GFF3047
GFF3048 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 483,415 to 484,116
GFF3048
Position (kb) 481
482
483 Strain fitness (log2 ratio) -2
-1
0
1 at 480.918 kb on - strand at 480.983 kb on + strand at 481.049 kb on + strand, within GFF3045 at 481.049 kb on + strand, within GFF3045 at 481.403 kb on + strand, within GFF3045 at 481.403 kb on + strand, within GFF3045 at 481.404 kb on - strand, within GFF3045 at 481.406 kb on + strand, within GFF3045 at 481.406 kb on + strand, within GFF3045 at 481.407 kb on - strand, within GFF3045 at 482.771 kb on + strand, within GFF3047 at 482.772 kb on - strand, within GFF3047 at 482.775 kb on - strand, within GFF3047 at 482.874 kb on - strand, within GFF3047 at 483.071 kb on + strand, within GFF3047 at 483.072 kb on - strand, within GFF3047 at 483.144 kb on - strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.188 kb on + strand, within GFF3047 at 483.390 kb on - strand at 483.491 kb on + strand, within GFF3048 at 483.492 kb on - strand, within GFF3048
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 480,918 - -0.8 480,983 + -1.0 481,049 + GFF3045 0.15 +0.0 481,049 + GFF3045 0.15 +0.2 481,403 + GFF3045 0.57 -0.8 481,403 + GFF3045 0.57 -1.3 481,404 - GFF3045 0.58 +0.0 481,406 + GFF3045 0.58 +0.8 481,406 + GFF3045 0.58 +0.4 481,407 - GFF3045 0.58 -0.4 482,771 + GFF3047 0.20 -0.9 482,772 - GFF3047 0.20 +0.2 482,775 - GFF3047 0.21 -0.0 482,874 - GFF3047 0.33 -0.8 483,071 + GFF3047 0.59 -0.4 483,072 - GFF3047 0.59 +1.5 483,144 - GFF3047 0.68 -0.6 483,188 + GFF3047 0.74 -2.0 483,188 + GFF3047 0.74 +1.1 483,188 + GFF3047 0.74 +0.5 483,188 + GFF3047 0.74 -0.5 483,188 + GFF3047 0.74 -0.4 483,390 - +0.5 483,491 + GFF3048 0.11 +1.4 483,492 - GFF3048 0.11 -0.8
Or see this region's nucleotide sequence