Strain Fitness in Variovorax sp. SCN45 around GFF3040

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3039 and GFF3040 are separated by 181 nucleotidesGFF3040 and GFF3041 are separated by 57 nucleotides GFF3039 - Two-component transcriptional response regulator, LuxR family, at 471,425 to 472,204 GFF3039 GFF3040 - hypothetical protein, at 472,386 to 474,662 GFF3040 GFF3041 - Outer membrane porin protein 32 precursor, at 474,720 to 475,877 GFF3041 Position (kb) 472 473 474 475Strain fitness (log2 ratio) -1 0 1at 471.465 kb on + strandat 471.466 kb on - strandat 471.466 kb on - strandat 471.466 kb on - strandat 471.741 kb on + strand, within GFF3039at 471.876 kb on + strand, within GFF3039at 471.876 kb on + strand, within GFF3039at 471.877 kb on - strand, within GFF3039at 471.877 kb on - strand, within GFF3039at 472.032 kb on + strand, within GFF3039at 472.204 kb on + strandat 472.204 kb on + strandat 472.205 kb on - strandat 472.258 kb on - strandat 473.135 kb on + strand, within GFF3040at 473.135 kb on + strand, within GFF3040at 473.135 kb on + strand, within GFF3040at 473.381 kb on - strand, within GFF3040at 473.384 kb on + strand, within GFF3040at 473.384 kb on + strand, within GFF3040at 473.385 kb on - strand, within GFF3040at 473.385 kb on - strand, within GFF3040at 473.703 kb on + strand, within GFF3040at 474.459 kb on + strandat 474.460 kb on - strandat 474.664 kb on - strandat 474.910 kb on + strand, within GFF3041at 475.406 kb on - strand, within GFF3041at 475.406 kb on - strand, within GFF3041at 475.406 kb on - strand, within GFF3041

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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471,465 + +1.0
471,466 - -0.5
471,466 - -0.1
471,466 - -0.5
471,741 + GFF3039 0.41 -0.4
471,876 + GFF3039 0.58 -0.3
471,876 + GFF3039 0.58 -1.0
471,877 - GFF3039 0.58 +1.5
471,877 - GFF3039 0.58 +1.5
472,032 + GFF3039 0.78 -1.3
472,204 + -0.6
472,204 + +0.0
472,205 - -0.2
472,258 - +0.1
473,135 + GFF3040 0.33 +0.7
473,135 + GFF3040 0.33 -0.1
473,135 + GFF3040 0.33 -0.2
473,381 - GFF3040 0.44 +1.0
473,384 + GFF3040 0.44 +0.4
473,384 + GFF3040 0.44 +1.5
473,385 - GFF3040 0.44 -0.3
473,385 - GFF3040 0.44 -0.9
473,703 + GFF3040 0.58 +1.0
474,459 + -1.0
474,460 - -0.3
474,664 - -0.3
474,910 + GFF3041 0.16 -0.2
475,406 - GFF3041 0.59 +0.7
475,406 - GFF3041 0.59 -0.2
475,406 - GFF3041 0.59 +0.3

Or see this region's nucleotide sequence