Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2984 and GFF2985 are separated by 8 nucleotides GFF2985 and GFF2986 are separated by 4 nucleotides GFF2986 and GFF2987 are separated by 30 nucleotides GFF2987 and GFF2988 are separated by 177 nucleotides
GFF2984 - Adenosine deaminase (EC 3.5.4.4), at 414,489 to 415,586
GFF2984
GFF2985 - BUG/TctC family periplasmic protein, at 415,595 to 416,572
GFF2985
GFF2986 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 416,577 to 417,674
GFF2986
GFF2987 - CBS domain protein, at 417,705 to 418,157
GFF2987
GFF2988 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) @ O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 418,335 to 419,639
GFF2988
Position (kb)
416
417
418 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 415.676 kb on - strand at 415.706 kb on + strand, within GFF2985 at 415.707 kb on - strand, within GFF2985 at 415.856 kb on + strand, within GFF2985 at 415.856 kb on + strand, within GFF2985 at 415.856 kb on + strand, within GFF2985 at 415.857 kb on - strand, within GFF2985 at 416.057 kb on + strand, within GFF2985 at 416.057 kb on + strand, within GFF2985 at 416.057 kb on + strand, within GFF2985 at 416.057 kb on + strand, within GFF2985 at 416.057 kb on + strand, within GFF2985 at 416.058 kb on - strand, within GFF2985 at 416.058 kb on - strand, within GFF2985 at 416.058 kb on - strand, within GFF2985 at 416.058 kb on - strand, within GFF2985 at 416.058 kb on - strand, within GFF2985 at 416.063 kb on + strand, within GFF2985 at 416.063 kb on + strand, within GFF2985 at 416.063 kb on + strand, within GFF2985 at 416.063 kb on + strand, within GFF2985 at 416.063 kb on + strand, within GFF2985 at 416.063 kb on + strand, within GFF2985 at 416.064 kb on - strand, within GFF2985 at 416.064 kb on - strand, within GFF2985 at 416.064 kb on - strand, within GFF2985 at 416.925 kb on + strand, within GFF2986 at 416.926 kb on - strand, within GFF2986 at 416.926 kb on - strand, within GFF2986 at 417.054 kb on + strand, within GFF2986 at 417.054 kb on + strand, within GFF2986 at 417.055 kb on - strand, within GFF2986 at 417.055 kb on - strand, within GFF2986 at 417.055 kb on - strand, within GFF2986 at 417.055 kb on - strand, within GFF2986 at 417.055 kb on - strand, within GFF2986 at 417.055 kb on - strand, within GFF2986 at 417.243 kb on + strand, within GFF2986 at 417.243 kb on + strand, within GFF2986 at 417.243 kb on + strand, within GFF2986 at 417.243 kb on + strand, within GFF2986 at 417.243 kb on + strand, within GFF2986 at 417.243 kb on + strand, within GFF2986 at 417.244 kb on - strand, within GFF2986 at 417.244 kb on - strand, within GFF2986 at 417.244 kb on - strand, within GFF2986 at 417.244 kb on - strand, within GFF2986 at 417.244 kb on - strand, within GFF2986 at 417.417 kb on + strand, within GFF2986 at 417.418 kb on - strand, within GFF2986 at 417.456 kb on + strand, within GFF2986 at 417.456 kb on + strand, within GFF2986 at 417.456 kb on + strand, within GFF2986 at 417.457 kb on - strand, within GFF2986 at 417.457 kb on - strand, within GFF2986 at 417.457 kb on - strand, within GFF2986 at 417.457 kb on - strand, within GFF2986 at 417.457 kb on - strand, within GFF2986 at 417.477 kb on + strand, within GFF2986 at 417.478 kb on - strand, within GFF2986 at 417.478 kb on - strand, within GFF2986 at 417.579 kb on + strand at 417.580 kb on - strand at 417.580 kb on - strand at 417.580 kb on - strand at 417.745 kb on + strand at 417.745 kb on + strand at 417.745 kb on + strand at 417.745 kb on + strand at 417.745 kb on + strand at 417.745 kb on + strand at 417.745 kb on + strand at 417.745 kb on + strand at 417.746 kb on - strand at 417.746 kb on - strand at 417.746 kb on - strand at 417.746 kb on - strand at 417.805 kb on + strand, within GFF2987 at 417.805 kb on + strand, within GFF2987 at 417.805 kb on + strand, within GFF2987 at 417.805 kb on + strand, within GFF2987 at 417.805 kb on + strand, within GFF2987 at 417.806 kb on - strand, within GFF2987 at 417.806 kb on - strand, within GFF2987 at 417.853 kb on + strand, within GFF2987 at 417.853 kb on + strand, within GFF2987 at 417.853 kb on + strand, within GFF2987 at 417.853 kb on + strand, within GFF2987 at 417.853 kb on + strand, within GFF2987 at 417.853 kb on + strand, within GFF2987 at 417.853 kb on + strand, within GFF2987 at 417.854 kb on - strand, within GFF2987 at 417.854 kb on - strand, within GFF2987 at 417.854 kb on - strand, within GFF2987 at 417.854 kb on - strand, within GFF2987 at 417.854 kb on - strand, within GFF2987 at 418.201 kb on + strand at 418.201 kb on + strand at 418.201 kb on + strand at 418.201 kb on + strand at 418.201 kb on + strand at 418.202 kb on - strand at 418.202 kb on - strand at 418.202 kb on - strand at 418.202 kb on - strand at 418.202 kb on - strand at 418.202 kb on - strand at 418.202 kb on - strand at 418.202 kb on - strand at 418.202 kb on - strand at 418.228 kb on + strand at 418.230 kb on + strand at 418.230 kb on + strand at 418.230 kb on + strand at 418.231 kb on - strand at 418.231 kb on - strand at 418.288 kb on + strand at 418.288 kb on + strand at 418.289 kb on - strand at 418.506 kb on + strand, within GFF2988 at 418.507 kb on - strand, within GFF2988 at 418.561 kb on + strand, within GFF2988 at 418.671 kb on + strand, within GFF2988
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 415,676 - +1.1 415,706 + GFF2985 0.11 -0.4 415,707 - GFF2985 0.11 -0.8 415,856 + GFF2985 0.27 -1.0 415,856 + GFF2985 0.27 -1.6 415,856 + GFF2985 0.27 -0.1 415,857 - GFF2985 0.27 -0.3 416,057 + GFF2985 0.47 -0.1 416,057 + GFF2985 0.47 +0.1 416,057 + GFF2985 0.47 -1.7 416,057 + GFF2985 0.47 +0.1 416,057 + GFF2985 0.47 -2.2 416,058 - GFF2985 0.47 +1.2 416,058 - GFF2985 0.47 +0.1 416,058 - GFF2985 0.47 -0.0 416,058 - GFF2985 0.47 -1.4 416,058 - GFF2985 0.47 -0.6 416,063 + GFF2985 0.48 -0.3 416,063 + GFF2985 0.48 +0.3 416,063 + GFF2985 0.48 +1.1 416,063 + GFF2985 0.48 +0.2 416,063 + GFF2985 0.48 -0.5 416,063 + GFF2985 0.48 -0.8 416,064 - GFF2985 0.48 -0.6 416,064 - GFF2985 0.48 -1.2 416,064 - GFF2985 0.48 -0.6 416,925 + GFF2986 0.32 -0.2 416,926 - GFF2986 0.32 -0.4 416,926 - GFF2986 0.32 -0.1 417,054 + GFF2986 0.43 -0.8 417,054 + GFF2986 0.43 -1.6 417,055 - GFF2986 0.44 -1.0 417,055 - GFF2986 0.44 -0.4 417,055 - GFF2986 0.44 +0.2 417,055 - GFF2986 0.44 +0.1 417,055 - GFF2986 0.44 -0.2 417,055 - GFF2986 0.44 -0.8 417,243 + GFF2986 0.61 +0.2 417,243 + GFF2986 0.61 -1.9 417,243 + GFF2986 0.61 -1.1 417,243 + GFF2986 0.61 +0.8 417,243 + GFF2986 0.61 +0.6 417,243 + GFF2986 0.61 -1.7 417,244 - GFF2986 0.61 +0.5 417,244 - GFF2986 0.61 -0.6 417,244 - GFF2986 0.61 -1.2 417,244 - GFF2986 0.61 -0.2 417,244 - GFF2986 0.61 -0.8 417,417 + GFF2986 0.77 -2.0 417,418 - GFF2986 0.77 -0.8 417,456 + GFF2986 0.80 -0.9 417,456 + GFF2986 0.80 +0.4 417,456 + GFF2986 0.80 -0.2 417,457 - GFF2986 0.80 +1.1 417,457 - GFF2986 0.80 -0.6 417,457 - GFF2986 0.80 +0.0 417,457 - GFF2986 0.80 +0.1 417,457 - GFF2986 0.80 -0.2 417,477 + GFF2986 0.82 +0.4 417,478 - GFF2986 0.82 +1.1 417,478 - GFF2986 0.82 -1.2 417,579 + +0.4 417,580 - -0.2 417,580 - -0.2 417,580 - -0.4 417,745 + -0.4 417,745 + -0.5 417,745 + +0.0 417,745 + -0.5 417,745 + +0.2 417,745 + -0.4 417,745 + -0.4 417,745 + -0.8 417,746 - -0.6 417,746 - -0.7 417,746 - +0.1 417,746 - -0.0 417,805 + GFF2987 0.22 +1.5 417,805 + GFF2987 0.22 +0.5 417,805 + GFF2987 0.22 -0.3 417,805 + GFF2987 0.22 -0.6 417,805 + GFF2987 0.22 -0.4 417,806 - GFF2987 0.22 -0.4 417,806 - GFF2987 0.22 -0.0 417,853 + GFF2987 0.33 +0.1 417,853 + GFF2987 0.33 -0.2 417,853 + GFF2987 0.33 -0.7 417,853 + GFF2987 0.33 -0.0 417,853 + GFF2987 0.33 -0.5 417,853 + GFF2987 0.33 -0.2 417,853 + GFF2987 0.33 -0.2 417,854 - GFF2987 0.33 -0.8 417,854 - GFF2987 0.33 -0.1 417,854 - GFF2987 0.33 +0.5 417,854 - GFF2987 0.33 +0.5 417,854 - GFF2987 0.33 -0.9 418,201 + +0.3 418,201 + +0.0 418,201 + -0.7 418,201 + -0.0 418,201 + -0.0 418,202 - -0.4 418,202 - -0.0 418,202 - -0.6 418,202 - -0.9 418,202 - +0.7 418,202 - -0.5 418,202 - -0.5 418,202 - -0.0 418,202 - +2.0 418,228 + -2.0 418,230 + +1.8 418,230 + -1.2 418,230 + -0.2 418,231 - +0.0 418,231 - +0.2 418,288 + -0.5 418,288 + +1.1 418,289 - -0.5 418,506 + GFF2988 0.13 +0.4 418,507 - GFF2988 0.13 -0.3 418,561 + GFF2988 0.17 -0.2 418,671 + GFF2988 0.26 -0.5
Or see this region's nucleotide sequence