Strain Fitness in Variovorax sp. SCN45 around GFF294

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF292 and GFF293 are separated by 198 nucleotidesGFF293 and GFF294 are separated by 27 nucleotidesGFF294 and GFF295 are separated by 22 nucleotides GFF292 - Tol-Pal system beta propeller repeat protein TolB, at 326,358 to 327,584 GFF292 GFF293 - no description, at 327,783 to 327,926 GFF293 GFF294 - Lipid A export permease/ATP-binding protein MsbA, at 327,954 to 329,621 GFF294 GFF295 - Ribonuclease G, at 329,644 to 331,170 GFF295 Position (kb) 327 328 329 330Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 326.977 kb on + strand, within GFF292at 326.977 kb on + strand, within GFF292at 326.978 kb on - strand, within GFF292at 326.978 kb on - strand, within GFF292at 326.978 kb on - strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.010 kb on + strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.011 kb on - strand, within GFF292at 327.118 kb on + strand, within GFF292at 327.142 kb on + strand, within GFF292at 327.142 kb on + strand, within GFF292at 327.142 kb on + strand, within GFF292at 327.143 kb on - strand, within GFF292at 327.190 kb on + strand, within GFF292at 327.190 kb on + strand, within GFF292at 327.190 kb on + strand, within GFF292at 327.190 kb on + strand, within GFF292at 327.190 kb on + strand, within GFF292at 327.191 kb on - strand, within GFF292at 327.191 kb on - strand, within GFF292at 327.191 kb on - strand, within GFF292at 327.191 kb on - strand, within GFF292at 327.191 kb on - strand, within GFF292at 327.196 kb on + strand, within GFF292at 327.196 kb on + strand, within GFF292at 327.196 kb on + strand, within GFF292at 327.196 kb on + strand, within GFF292at 327.196 kb on + strand, within GFF292at 327.196 kb on + strand, within GFF292at 327.197 kb on - strand, within GFF292at 327.197 kb on - strand, within GFF292at 327.197 kb on - strand, within GFF292at 327.197 kb on - strand, within GFF292at 327.247 kb on + strand, within GFF292at 327.247 kb on + strand, within GFF292at 327.247 kb on + strand, within GFF292at 327.247 kb on + strand, within GFF292at 327.247 kb on + strand, within GFF292at 327.248 kb on - strand, within GFF292at 327.248 kb on - strand, within GFF292at 327.248 kb on - strand, within GFF292at 327.248 kb on - strand, within GFF292at 327.248 kb on - strand, within GFF292at 327.248 kb on - strand, within GFF292at 327.335 kb on + strand, within GFF292at 327.423 kb on + strand, within GFF292at 327.423 kb on + strand, within GFF292at 327.423 kb on + strand, within GFF292at 327.423 kb on + strand, within GFF292at 327.627 kb on + strandat 327.627 kb on + strandat 327.627 kb on + strandat 327.627 kb on + strandat 327.627 kb on + strandat 327.628 kb on - strandat 327.628 kb on - strandat 327.628 kb on - strandat 327.628 kb on - strandat 327.731 kb on + strandat 327.731 kb on + strandat 327.731 kb on + strandat 327.731 kb on + strandat 327.731 kb on + strandat 327.731 kb on + strandat 327.731 kb on + strandat 327.731 kb on + strandat 327.732 kb on - strandat 327.732 kb on - strandat 327.732 kb on - strandat 327.732 kb on - strandat 327.767 kb on - strandat 327.784 kb on + strandat 327.785 kb on - strandat 327.785 kb on - strandat 327.785 kb on - strandat 327.823 kb on + strand, within GFF293at 327.823 kb on + strand, within GFF293at 327.824 kb on - strand, within GFF293at 327.824 kb on - strand, within GFF293at 329.664 kb on - strandat 329.664 kb on - strandat 329.664 kb on - strandat 329.664 kb on - strandat 329.849 kb on + strand, within GFF295at 329.850 kb on - strand, within GFF295at 330.305 kb on + strand, within GFF295at 330.306 kb on - strand, within GFF295at 330.306 kb on - strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.335 kb on + strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.336 kb on - strand, within GFF295at 330.551 kb on + strand, within GFF295at 330.551 kb on + strand, within GFF295at 330.551 kb on + strand, within GFF295at 330.552 kb on - strand, within GFF295at 330.552 kb on - strand, within GFF295

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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326,977 + GFF292 0.50 -0.5
326,977 + GFF292 0.50 +1.2
326,978 - GFF292 0.51 +1.2
326,978 - GFF292 0.51 -1.8
326,978 - GFF292 0.51 -0.4
327,010 + GFF292 0.53 -2.0
327,010 + GFF292 0.53 -1.1
327,010 + GFF292 0.53 +2.2
327,010 + GFF292 0.53 -2.0
327,010 + GFF292 0.53 -1.1
327,010 + GFF292 0.53 -2.3
327,010 + GFF292 0.53 -1.6
327,010 + GFF292 0.53 -2.0
327,010 + GFF292 0.53 +0.2
327,010 + GFF292 0.53 -2.9
327,010 + GFF292 0.53 -0.5
327,010 + GFF292 0.53 -2.2
327,010 + GFF292 0.53 -1.4
327,010 + GFF292 0.53 +0.2
327,011 - GFF292 0.53 -2.1
327,011 - GFF292 0.53 -0.8
327,011 - GFF292 0.53 -0.6
327,011 - GFF292 0.53 -1.2
327,011 - GFF292 0.53 -1.4
327,011 - GFF292 0.53 -1.8
327,011 - GFF292 0.53 -0.7
327,011 - GFF292 0.53 -1.2
327,011 - GFF292 0.53 +0.2
327,011 - GFF292 0.53 -0.2
327,011 - GFF292 0.53 -2.7
327,011 - GFF292 0.53 -2.1
327,011 - GFF292 0.53 -0.4
327,011 - GFF292 0.53 -1.7
327,011 - GFF292 0.53 -1.5
327,011 - GFF292 0.53 -1.3
327,011 - GFF292 0.53 +0.4
327,118 + GFF292 0.62 -0.1
327,142 + GFF292 0.64 -0.4
327,142 + GFF292 0.64 -0.4
327,142 + GFF292 0.64 -0.4
327,143 - GFF292 0.64 -0.5
327,190 + GFF292 0.68 -0.4
327,190 + GFF292 0.68 -0.4
327,190 + GFF292 0.68 -3.4
327,190 + GFF292 0.68 -1.2
327,190 + GFF292 0.68 -0.5
327,191 - GFF292 0.68 -1.0
327,191 - GFF292 0.68 -0.1
327,191 - GFF292 0.68 -0.2
327,191 - GFF292 0.68 +0.1
327,191 - GFF292 0.68 -1.4
327,196 + GFF292 0.68 -1.8
327,196 + GFF292 0.68 -2.4
327,196 + GFF292 0.68 +0.0
327,196 + GFF292 0.68 -0.0
327,196 + GFF292 0.68 -0.5
327,196 + GFF292 0.68 -1.3
327,197 - GFF292 0.68 -1.0
327,197 - GFF292 0.68 +0.2
327,197 - GFF292 0.68 -1.9
327,197 - GFF292 0.68 -2.0
327,247 + GFF292 0.72 -1.4
327,247 + GFF292 0.72 +0.7
327,247 + GFF292 0.72 +0.1
327,247 + GFF292 0.72 -2.0
327,247 + GFF292 0.72 -1.7
327,248 - GFF292 0.73 -1.4
327,248 - GFF292 0.73 -0.1
327,248 - GFF292 0.73 -2.5
327,248 - GFF292 0.73 +1.2
327,248 - GFF292 0.73 -1.7
327,248 - GFF292 0.73 -2.3
327,335 + GFF292 0.80 -2.0
327,423 + GFF292 0.87 -0.2
327,423 + GFF292 0.87 -1.4
327,423 + GFF292 0.87 -1.3
327,423 + GFF292 0.87 -0.4
327,627 + -0.6
327,627 + +0.6
327,627 + +0.3
327,627 + -0.0
327,627 + -0.5
327,628 - +0.1
327,628 - +0.1
327,628 - -0.7
327,628 - -0.2
327,731 + -0.3
327,731 + -1.2
327,731 + +0.4
327,731 + +1.0
327,731 + -0.8
327,731 + -0.9
327,731 + -0.1
327,731 + -0.5
327,732 - -0.0
327,732 - -0.0
327,732 - -0.8
327,732 - -0.2
327,767 - -0.3
327,784 + -0.0
327,785 - -0.6
327,785 - -0.4
327,785 - -0.1
327,823 + GFF293 0.28 -0.2
327,823 + GFF293 0.28 +0.2
327,824 - GFF293 0.28 +1.2
327,824 - GFF293 0.28 +0.9
329,664 - -0.9
329,664 - +0.0
329,664 - +0.0
329,664 - -0.0
329,849 + GFF295 0.13 +1.2
329,850 - GFF295 0.13 -0.8
330,305 + GFF295 0.43 -1.2
330,306 - GFF295 0.43 +0.7
330,306 - GFF295 0.43 +0.2
330,335 + GFF295 0.45 +0.9
330,335 + GFF295 0.45 -0.2
330,335 + GFF295 0.45 -0.2
330,335 + GFF295 0.45 -1.1
330,335 + GFF295 0.45 -1.8
330,335 + GFF295 0.45 -0.1
330,335 + GFF295 0.45 -1.0
330,335 + GFF295 0.45 -0.1
330,335 + GFF295 0.45 +0.1
330,336 - GFF295 0.45 -0.4
330,336 - GFF295 0.45 -0.1
330,336 - GFF295 0.45 +1.0
330,336 - GFF295 0.45 -0.0
330,336 - GFF295 0.45 +0.5
330,336 - GFF295 0.45 -0.6
330,336 - GFF295 0.45 +0.6
330,336 - GFF295 0.45 +0.3
330,336 - GFF295 0.45 -0.6
330,551 + GFF295 0.59 -1.3
330,551 + GFF295 0.59 -0.6
330,551 + GFF295 0.59 -0.1
330,552 - GFF295 0.59 +1.6
330,552 - GFF295 0.59 -1.2

Or see this region's nucleotide sequence