Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF28 and GFF29 are separated by 145 nucleotides GFF29 and GFF30 are separated by 117 nucleotides
GFF28 - hypothetical protein, at 32,350 to 33,246
GFF28
GFF29 - tRNA-modifying protein YgfZ, at 33,392 to 34,327
GFF29
GFF30 - Murein endolytic transglycosylase MltG, at 34,445 to 35,467
GFF30
Position (kb)
33
34
35 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 32.480 kb on + strand, within GFF28 at 32.481 kb on - strand, within GFF28 at 32.543 kb on + strand, within GFF28 at 32.543 kb on + strand, within GFF28 at 32.543 kb on + strand, within GFF28 at 32.543 kb on + strand, within GFF28 at 32.543 kb on + strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.544 kb on - strand, within GFF28 at 32.918 kb on + strand, within GFF28 at 32.918 kb on + strand, within GFF28 at 32.918 kb on + strand, within GFF28 at 32.918 kb on + strand, within GFF28 at 32.918 kb on + strand, within GFF28 at 32.919 kb on - strand, within GFF28 at 32.919 kb on - strand, within GFF28 at 32.919 kb on - strand, within GFF28 at 32.919 kb on - strand, within GFF28 at 33.026 kb on + strand, within GFF28 at 33.027 kb on - strand, within GFF28 at 33.027 kb on - strand, within GFF28 at 33.056 kb on + strand, within GFF28 at 33.056 kb on + strand, within GFF28 at 33.057 kb on - strand, within GFF28 at 33.057 kb on - strand, within GFF28 at 33.057 kb on - strand, within GFF28 at 33.074 kb on + strand, within GFF28 at 33.161 kb on + strand at 33.162 kb on - strand at 33.162 kb on - strand at 33.162 kb on - strand at 33.286 kb on - strand at 33.429 kb on - strand at 34.336 kb on + strand at 34.336 kb on + strand at 34.931 kb on + strand, within GFF30 at 34.931 kb on + strand, within GFF30 at 34.937 kb on + strand, within GFF30 at 35.183 kb on + strand, within GFF30 at 35.183 kb on + strand, within GFF30 at 35.183 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 32,480 + GFF28 0.14 -0.5 32,481 - GFF28 0.15 -0.4 32,543 + GFF28 0.22 -0.7 32,543 + GFF28 0.22 -0.6 32,543 + GFF28 0.22 -0.4 32,543 + GFF28 0.22 -0.3 32,543 + GFF28 0.22 -1.1 32,544 - GFF28 0.22 -0.4 32,544 - GFF28 0.22 +0.6 32,544 - GFF28 0.22 +0.6 32,544 - GFF28 0.22 -0.4 32,544 - GFF28 0.22 -0.0 32,544 - GFF28 0.22 -0.4 32,544 - GFF28 0.22 -1.2 32,544 - GFF28 0.22 -0.8 32,544 - GFF28 0.22 -1.4 32,544 - GFF28 0.22 -0.5 32,918 + GFF28 0.63 +0.7 32,918 + GFF28 0.63 +0.6 32,918 + GFF28 0.63 +0.9 32,918 + GFF28 0.63 -1.6 32,918 + GFF28 0.63 -0.4 32,919 - GFF28 0.63 -0.5 32,919 - GFF28 0.63 +2.4 32,919 - GFF28 0.63 -0.2 32,919 - GFF28 0.63 -1.1 33,026 + GFF28 0.75 -0.3 33,027 - GFF28 0.75 -1.4 33,027 - GFF28 0.75 +0.1 33,056 + GFF28 0.79 +0.1 33,056 + GFF28 0.79 -0.1 33,057 - GFF28 0.79 -0.8 33,057 - GFF28 0.79 +0.3 33,057 - GFF28 0.79 -1.6 33,074 + GFF28 0.81 -2.4 33,161 + +0.6 33,162 - -0.4 33,162 - +0.5 33,162 - +0.2 33,286 - -0.8 33,429 - -2.5 34,336 + -0.5 34,336 + -0.1 34,931 + GFF30 0.48 -1.2 34,931 + GFF30 0.48 -0.8 34,937 + GFF30 0.48 +0.8 35,183 + GFF30 0.72 +1.2 35,183 + GFF30 0.72 -1.5 35,183 + GFF30 0.72 -0.0 35,255 + GFF30 0.79 -1.1 35,255 + GFF30 0.79 +1.2 35,255 + GFF30 0.79 -0.5 35,255 + GFF30 0.79 -2.0 35,255 + GFF30 0.79 +0.6 35,255 + GFF30 0.79 -0.3 35,255 + GFF30 0.79 +0.2 35,255 + GFF30 0.79 -1.0 35,255 + GFF30 0.79 -0.6 35,255 + GFF30 0.79 -0.4
Or see this region's nucleotide sequence