Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2702 and GFF2703 are separated by 65 nucleotides GFF2703 and GFF2704 are separated by 10 nucleotides GFF2704 and GFF2705 are separated by 6 nucleotides
GFF2702 - FAD-dependent oxidoreductase, at 129,557 to 130,939
GFF2702
GFF2703 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 131,005 to 131,868
GFF2703
GFF2704 - Hydroxymethylpyrimidine ABC transporter, ATP-binding protein, at 131,879 to 132,673
GFF2704
GFF2705 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 132,680 to 133,711
GFF2705
Position (kb) 131
132 Strain fitness (log2 ratio) -2
-1
0
1 at 130.296 kb on + strand, within GFF2702 at 130.297 kb on - strand, within GFF2702 at 130.297 kb on - strand, within GFF2702 at 130.297 kb on - strand, within GFF2702 at 130.578 kb on + strand, within GFF2702 at 130.578 kb on + strand, within GFF2702 at 130.579 kb on - strand, within GFF2702 at 130.579 kb on - strand, within GFF2702 at 130.785 kb on + strand, within GFF2702 at 130.786 kb on - strand, within GFF2702 at 130.930 kb on + strand at 130.940 kb on - strand at 131.627 kb on + strand, within GFF2703 at 132.749 kb on - strand at 132.784 kb on - strand, within GFF2705 at 132.861 kb on + strand, within GFF2705 at 132.861 kb on + strand, within GFF2705
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 130,296 + GFF2702 0.53 -0.3 130,297 - GFF2702 0.54 -1.1 130,297 - GFF2702 0.54 -0.2 130,297 - GFF2702 0.54 -2.1 130,578 + GFF2702 0.74 +0.9 130,578 + GFF2702 0.74 +0.0 130,579 - GFF2702 0.74 -0.1 130,579 - GFF2702 0.74 -1.2 130,785 + GFF2702 0.89 -1.0 130,786 - GFF2702 0.89 -1.1 130,930 + +0.8 130,940 - -0.6 131,627 + GFF2703 0.72 -1.9 132,749 - -1.9 132,784 - GFF2705 0.10 -0.1 132,861 + GFF2705 0.18 -0.0 132,861 + GFF2705 0.18 -0.0
Or see this region's nucleotide sequence