Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2688 and GFF2689 are separated by 35 nucleotides GFF2689 and GFF2690 overlap by 4 nucleotides GFF2690 and GFF2691 are separated by 89 nucleotides GFF2691 and GFF2692 overlap by 4 nucleotides
GFF2688 - Glutamine ABC transporter, substrate-binding protein GlnH, at 117,899 to 118,723
GFF2688
GFF2689 - Protein containing transglutaminase-like domain, putative cysteine protease, at 118,759 to 119,715
GFF2689
GFF2690 - RidA/YER057c/UK114 superfamily, group 1, at 119,712 to 120,170
GFF2690
GFF2691 - no description, at 120,260 to 120,934
GFF2691
GFF2692 - hypothetical protein, at 120,931 to 121,506
GFF2692
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 118.795 kb on + strand at 118.927 kb on + strand, within GFF2689 at 119.131 kb on + strand, within GFF2689 at 119.131 kb on + strand, within GFF2689 at 119.231 kb on - strand, within GFF2689 at 119.272 kb on - strand, within GFF2689 at 119.371 kb on + strand, within GFF2689 at 119.536 kb on + strand, within GFF2689 at 119.537 kb on - strand, within GFF2689 at 119.537 kb on - strand, within GFF2689 at 119.537 kb on - strand, within GFF2689 at 119.564 kb on - strand, within GFF2689 at 119.591 kb on + strand, within GFF2689 at 119.592 kb on - strand, within GFF2689 at 119.782 kb on - strand, within GFF2690 at 119.851 kb on + strand, within GFF2690 at 120.217 kb on + strand at 120.217 kb on + strand at 120.218 kb on - strand at 120.360 kb on + strand, within GFF2691 at 120.361 kb on - strand, within GFF2691 at 120.361 kb on - strand, within GFF2691 at 120.749 kb on + strand, within GFF2691 at 120.749 kb on + strand, within GFF2691 at 120.750 kb on - strand, within GFF2691 at 120.782 kb on + strand, within GFF2691 at 120.867 kb on - strand at 120.875 kb on + strand at 120.875 kb on + strand at 120.876 kb on - strand at 121.115 kb on - strand, within GFF2692
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 118,795 + +0.6 118,927 + GFF2689 0.18 -0.4 119,131 + GFF2689 0.39 -0.1 119,131 + GFF2689 0.39 +0.0 119,231 - GFF2689 0.49 -0.6 119,272 - GFF2689 0.54 -2.2 119,371 + GFF2689 0.64 -0.3 119,536 + GFF2689 0.81 -0.2 119,537 - GFF2689 0.81 +0.1 119,537 - GFF2689 0.81 +2.0 119,537 - GFF2689 0.81 -0.7 119,564 - GFF2689 0.84 -0.0 119,591 + GFF2689 0.87 -0.7 119,592 - GFF2689 0.87 +0.7 119,782 - GFF2690 0.15 +0.8 119,851 + GFF2690 0.30 -0.4 120,217 + -0.3 120,217 + +0.9 120,218 - -1.0 120,360 + GFF2691 0.15 -1.6 120,361 - GFF2691 0.15 +1.5 120,361 - GFF2691 0.15 +0.5 120,749 + GFF2691 0.72 -0.8 120,749 + GFF2691 0.72 +1.9 120,750 - GFF2691 0.73 -0.5 120,782 + GFF2691 0.77 +0.5 120,867 - -0.3 120,875 + -0.1 120,875 + +0.3 120,876 - -0.7 121,115 - GFF2692 0.32 -0.2
Or see this region's nucleotide sequence