Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2657 and GFF2658 are separated by 79 nucleotides GFF2658 and GFF2659 are separated by 74 nucleotides
GFF2657 - putative oxidoreductase, nitronate monooxygenase family, at 78,505 to 79,467
GFF2657
GFF2658 - 3-methylmercaptopropionyl-CoA dehydrogenase (DmdC), at 79,547 to 81,337
GFF2658
GFF2659 - Electron transfer flavoprotein, alpha subunit, at 81,412 to 82,344
GFF2659
Position (kb) 79
80
81
82 Strain fitness (log2 ratio) -2
-1
0
1 at 78.734 kb on + strand, within GFF2657 at 78.735 kb on - strand, within GFF2657 at 78.809 kb on + strand, within GFF2657 at 78.809 kb on + strand, within GFF2657 at 78.809 kb on + strand, within GFF2657 at 78.809 kb on + strand, within GFF2657 at 78.810 kb on - strand, within GFF2657 at 79.101 kb on - strand, within GFF2657 at 79.101 kb on - strand, within GFF2657 at 79.101 kb on - strand, within GFF2657 at 79.101 kb on - strand, within GFF2657 at 79.166 kb on + strand, within GFF2657 at 79.166 kb on + strand, within GFF2657 at 79.166 kb on + strand, within GFF2657 at 79.166 kb on + strand, within GFF2657 at 79.167 kb on - strand, within GFF2657 at 79.271 kb on + strand, within GFF2657 at 79.271 kb on + strand, within GFF2657 at 79.496 kb on + strand at 79.496 kb on + strand at 79.642 kb on - strand at 79.642 kb on - strand at 79.642 kb on - strand at 79.770 kb on + strand, within GFF2658 at 79.770 kb on + strand, within GFF2658 at 80.025 kb on + strand, within GFF2658 at 80.025 kb on + strand, within GFF2658 at 80.025 kb on + strand, within GFF2658 at 80.025 kb on + strand, within GFF2658 at 80.026 kb on - strand, within GFF2658 at 80.026 kb on - strand, within GFF2658 at 80.026 kb on - strand, within GFF2658 at 80.055 kb on + strand, within GFF2658 at 80.055 kb on + strand, within GFF2658 at 80.055 kb on + strand, within GFF2658 at 80.056 kb on - strand, within GFF2658 at 80.056 kb on - strand, within GFF2658 at 80.146 kb on - strand, within GFF2658 at 80.176 kb on - strand, within GFF2658 at 80.176 kb on - strand, within GFF2658 at 80.232 kb on + strand, within GFF2658 at 80.403 kb on + strand, within GFF2658 at 80.403 kb on + strand, within GFF2658 at 80.404 kb on - strand, within GFF2658 at 80.446 kb on - strand, within GFF2658 at 80.475 kb on + strand, within GFF2658 at 80.475 kb on + strand, within GFF2658 at 80.475 kb on + strand, within GFF2658 at 80.475 kb on + strand, within GFF2658 at 80.476 kb on - strand, within GFF2658 at 80.476 kb on - strand, within GFF2658 at 80.476 kb on - strand, within GFF2658 at 80.476 kb on - strand, within GFF2658 at 80.509 kb on - strand, within GFF2658 at 80.745 kb on + strand, within GFF2658 at 80.745 kb on + strand, within GFF2658 at 80.746 kb on - strand, within GFF2658 at 80.746 kb on - strand, within GFF2658 at 80.746 kb on - strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.778 kb on + strand, within GFF2658 at 80.779 kb on - strand, within GFF2658 at 80.779 kb on - strand, within GFF2658 at 80.779 kb on - strand, within GFF2658 at 80.779 kb on - strand, within GFF2658 at 80.779 kb on - strand, within GFF2658 at 81.087 kb on + strand, within GFF2658 at 81.087 kb on + strand, within GFF2658 at 81.088 kb on - strand, within GFF2658 at 81.088 kb on - strand, within GFF2658 at 81.088 kb on - strand, within GFF2658 at 81.088 kb on - strand, within GFF2658 at 81.330 kb on + strand at 81.330 kb on + strand at 81.331 kb on - strand at 81.331 kb on - strand at 81.352 kb on + strand at 81.352 kb on + strand at 81.352 kb on + strand at 81.353 kb on - strand at 81.638 kb on + strand, within GFF2659 at 81.638 kb on + strand, within GFF2659 at 82.094 kb on + strand, within GFF2659 at 82.094 kb on + strand, within GFF2659 at 82.094 kb on + strand, within GFF2659
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 78,734 + GFF2657 0.24 -0.5 78,735 - GFF2657 0.24 -0.8 78,809 + GFF2657 0.32 -0.4 78,809 + GFF2657 0.32 +0.1 78,809 + GFF2657 0.32 +0.3 78,809 + GFF2657 0.32 -1.1 78,810 - GFF2657 0.32 +1.1 79,101 - GFF2657 0.62 -0.9 79,101 - GFF2657 0.62 +0.7 79,101 - GFF2657 0.62 -0.4 79,101 - GFF2657 0.62 +0.7 79,166 + GFF2657 0.69 -0.6 79,166 + GFF2657 0.69 +0.3 79,166 + GFF2657 0.69 -0.9 79,166 + GFF2657 0.69 -0.6 79,167 - GFF2657 0.69 +0.1 79,271 + GFF2657 0.80 -0.3 79,271 + GFF2657 0.80 +0.5 79,496 + -1.0 79,496 + -1.2 79,642 - -0.5 79,642 - -0.3 79,642 - +0.4 79,770 + GFF2658 0.12 -0.5 79,770 + GFF2658 0.12 -0.6 80,025 + GFF2658 0.27 -1.3 80,025 + GFF2658 0.27 -0.4 80,025 + GFF2658 0.27 +0.7 80,025 + GFF2658 0.27 -0.1 80,026 - GFF2658 0.27 +0.0 80,026 - GFF2658 0.27 -0.6 80,026 - GFF2658 0.27 +0.1 80,055 + GFF2658 0.28 -0.7 80,055 + GFF2658 0.28 -0.9 80,055 + GFF2658 0.28 +0.4 80,056 - GFF2658 0.28 +0.2 80,056 - GFF2658 0.28 +0.5 80,146 - GFF2658 0.33 +0.5 80,176 - GFF2658 0.35 -1.9 80,176 - GFF2658 0.35 -0.3 80,232 + GFF2658 0.38 -0.5 80,403 + GFF2658 0.48 +0.2 80,403 + GFF2658 0.48 -0.3 80,404 - GFF2658 0.48 -0.5 80,446 - GFF2658 0.50 -0.1 80,475 + GFF2658 0.52 -0.7 80,475 + GFF2658 0.52 -0.2 80,475 + GFF2658 0.52 -1.6 80,475 + GFF2658 0.52 +0.3 80,476 - GFF2658 0.52 -0.5 80,476 - GFF2658 0.52 -0.6 80,476 - GFF2658 0.52 +0.1 80,476 - GFF2658 0.52 -0.2 80,509 - GFF2658 0.54 -0.1 80,745 + GFF2658 0.67 +1.1 80,745 + GFF2658 0.67 -0.4 80,746 - GFF2658 0.67 -0.0 80,746 - GFF2658 0.67 -0.5 80,746 - GFF2658 0.67 -0.2 80,778 + GFF2658 0.69 +0.0 80,778 + GFF2658 0.69 +0.5 80,778 + GFF2658 0.69 +0.7 80,778 + GFF2658 0.69 -0.1 80,778 + GFF2658 0.69 -0.6 80,778 + GFF2658 0.69 -0.5 80,778 + GFF2658 0.69 -0.2 80,778 + GFF2658 0.69 +0.2 80,778 + GFF2658 0.69 -0.0 80,778 + GFF2658 0.69 +0.4 80,778 + GFF2658 0.69 -0.2 80,779 - GFF2658 0.69 -0.0 80,779 - GFF2658 0.69 +0.2 80,779 - GFF2658 0.69 +1.1 80,779 - GFF2658 0.69 -0.1 80,779 - GFF2658 0.69 +1.4 81,087 + GFF2658 0.86 -1.2 81,087 + GFF2658 0.86 -0.3 81,088 - GFF2658 0.86 -1.7 81,088 - GFF2658 0.86 -0.1 81,088 - GFF2658 0.86 -0.4 81,088 - GFF2658 0.86 +1.0 81,330 + -0.1 81,330 + -0.7 81,331 - +1.5 81,331 - -0.4 81,352 + -0.1 81,352 + -0.4 81,352 + -0.2 81,353 - +0.5 81,638 + GFF2659 0.24 -2.4 81,638 + GFF2659 0.24 -2.0 82,094 + GFF2659 0.73 -2.6 82,094 + GFF2659 0.73 -0.9 82,094 + GFF2659 0.73 -1.2
Or see this region's nucleotide sequence