Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF256 and GFF257 are separated by 16 nucleotides GFF257 and GFF258 overlap by 4 nucleotides
GFF256 - Twitching motility protein PilT, at 287,919 to 289,067
GFF256
GFF257 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 289,084 to 290,265
GFF257
GFF258 - Ribosomal protein L3 N(5)-glutamine methyltransferase (EC 2.1.1.298), at 290,262 to 291,140
GFF258
Position (kb)
289
290
291 Strain fitness (log2 ratio)
-2
-1
0
1 at 288.162 kb on + strand, within GFF256 at 288.162 kb on + strand, within GFF256 at 288.162 kb on + strand, within GFF256 at 288.162 kb on + strand, within GFF256 at 288.162 kb on + strand, within GFF256 at 288.163 kb on - strand, within GFF256 at 288.163 kb on - strand, within GFF256 at 288.163 kb on - strand, within GFF256 at 288.163 kb on - strand, within GFF256 at 288.163 kb on - strand, within GFF256 at 288.163 kb on - strand, within GFF256 at 288.163 kb on - strand, within GFF256 at 288.163 kb on - strand, within GFF256 at 288.168 kb on - strand, within GFF256 at 288.168 kb on - strand, within GFF256 at 288.195 kb on + strand, within GFF256 at 288.195 kb on + strand, within GFF256 at 288.195 kb on + strand, within GFF256 at 288.195 kb on + strand, within GFF256 at 288.195 kb on + strand, within GFF256 at 288.195 kb on + strand, within GFF256 at 288.195 kb on + strand, within GFF256 at 288.196 kb on - strand, within GFF256 at 288.196 kb on - strand, within GFF256 at 288.196 kb on - strand, within GFF256 at 288.196 kb on - strand, within GFF256 at 288.213 kb on + strand, within GFF256 at 288.213 kb on + strand, within GFF256 at 288.213 kb on + strand, within GFF256 at 288.213 kb on + strand, within GFF256 at 288.214 kb on - strand, within GFF256 at 288.214 kb on - strand, within GFF256 at 288.214 kb on - strand, within GFF256 at 288.214 kb on - strand, within GFF256 at 288.214 kb on - strand, within GFF256 at 288.357 kb on + strand, within GFF256 at 288.357 kb on + strand, within GFF256 at 288.409 kb on - strand, within GFF256 at 288.417 kb on + strand, within GFF256 at 288.417 kb on + strand, within GFF256 at 288.417 kb on + strand, within GFF256 at 288.417 kb on + strand, within GFF256 at 288.417 kb on + strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.418 kb on - strand, within GFF256 at 288.570 kb on + strand, within GFF256 at 288.570 kb on + strand, within GFF256 at 288.570 kb on + strand, within GFF256 at 288.570 kb on + strand, within GFF256 at 288.571 kb on - strand, within GFF256 at 288.621 kb on + strand, within GFF256 at 288.621 kb on + strand, within GFF256 at 288.621 kb on + strand, within GFF256 at 288.621 kb on + strand, within GFF256 at 288.622 kb on - strand, within GFF256 at 288.622 kb on - strand, within GFF256 at 288.651 kb on + strand, within GFF256 at 288.651 kb on + strand, within GFF256 at 288.688 kb on + strand, within GFF256 at 288.688 kb on + strand, within GFF256 at 288.688 kb on + strand, within GFF256 at 288.688 kb on + strand, within GFF256 at 288.689 kb on - strand, within GFF256 at 288.689 kb on - strand, within GFF256 at 288.689 kb on - strand, within GFF256 at 289.065 kb on + strand at 289.065 kb on + strand at 290.499 kb on + strand, within GFF258 at 290.499 kb on + strand, within GFF258 at 290.499 kb on + strand, within GFF258 at 290.500 kb on - strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.538 kb on + strand, within GFF258 at 290.539 kb on - strand, within GFF258 at 290.539 kb on - strand, within GFF258 at 290.539 kb on - strand, within GFF258 at 290.539 kb on - strand, within GFF258 at 290.539 kb on - strand, within GFF258 at 290.539 kb on - strand, within GFF258 at 290.608 kb on - strand, within GFF258 at 290.608 kb on - strand, within GFF258 at 290.688 kb on + strand, within GFF258 at 290.689 kb on - strand, within GFF258 at 290.826 kb on + strand, within GFF258 at 290.826 kb on + strand, within GFF258 at 290.827 kb on - strand, within GFF258 at 290.853 kb on + strand, within GFF258 at 290.853 kb on + strand, within GFF258 at 290.853 kb on + strand, within GFF258 at 290.853 kb on + strand, within GFF258 at 290.853 kb on + strand, within GFF258 at 290.853 kb on + strand, within GFF258 at 290.853 kb on + strand, within GFF258 at 290.853 kb on + strand, within GFF258 at 290.854 kb on - strand, within GFF258 at 290.854 kb on - strand, within GFF258 at 291.027 kb on + strand, within GFF258 at 291.027 kb on + strand, within GFF258 at 291.027 kb on + strand, within GFF258 at 291.028 kb on - strand, within GFF258 at 291.028 kb on - strand, within GFF258 at 291.028 kb on - strand, within GFF258 at 291.028 kb on - strand, within GFF258 at 291.028 kb on - strand, within GFF258 at 291.028 kb on - strand, within GFF258 at 291.028 kb on - strand, within GFF258 at 291.225 kb on + strand at 291.225 kb on + strand at 291.226 kb on - strand at 291.247 kb on + strand at 291.248 kb on - strand at 291.248 kb on - strand at 291.248 kb on - strand at 291.248 kb on - strand at 291.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 288,162 + GFF256 0.21 +1.2 288,162 + GFF256 0.21 -0.9 288,162 + GFF256 0.21 +1.4 288,162 + GFF256 0.21 +0.3 288,162 + GFF256 0.21 -1.1 288,163 - GFF256 0.21 +1.0 288,163 - GFF256 0.21 -0.5 288,163 - GFF256 0.21 -0.4 288,163 - GFF256 0.21 +0.0 288,163 - GFF256 0.21 -0.9 288,163 - GFF256 0.21 -0.0 288,163 - GFF256 0.21 -1.5 288,163 - GFF256 0.21 -0.1 288,168 - GFF256 0.22 -1.3 288,168 - GFF256 0.22 -1.8 288,195 + GFF256 0.24 +0.4 288,195 + GFF256 0.24 -0.2 288,195 + GFF256 0.24 +0.2 288,195 + GFF256 0.24 -1.6 288,195 + GFF256 0.24 +1.2 288,195 + GFF256 0.24 -0.6 288,195 + GFF256 0.24 -0.7 288,196 - GFF256 0.24 -0.7 288,196 - GFF256 0.24 -0.6 288,196 - GFF256 0.24 +1.1 288,196 - GFF256 0.24 -0.0 288,213 + GFF256 0.26 +0.3 288,213 + GFF256 0.26 -0.4 288,213 + GFF256 0.26 +0.3 288,213 + GFF256 0.26 +0.2 288,214 - GFF256 0.26 +0.5 288,214 - GFF256 0.26 +0.1 288,214 - GFF256 0.26 -0.8 288,214 - GFF256 0.26 -0.5 288,214 - GFF256 0.26 +0.2 288,357 + GFF256 0.38 +0.2 288,357 + GFF256 0.38 -0.3 288,409 - GFF256 0.43 -0.8 288,417 + GFF256 0.43 +0.9 288,417 + GFF256 0.43 +0.5 288,417 + GFF256 0.43 +1.0 288,417 + GFF256 0.43 -1.5 288,417 + GFF256 0.43 -1.5 288,418 - GFF256 0.43 -0.1 288,418 - GFF256 0.43 +0.3 288,418 - GFF256 0.43 -0.3 288,418 - GFF256 0.43 -0.3 288,418 - GFF256 0.43 -0.8 288,418 - GFF256 0.43 -0.3 288,418 - GFF256 0.43 -1.1 288,418 - GFF256 0.43 -0.8 288,418 - GFF256 0.43 +0.8 288,418 - GFF256 0.43 -2.4 288,418 - GFF256 0.43 +0.6 288,418 - GFF256 0.43 +0.7 288,570 + GFF256 0.57 -0.5 288,570 + GFF256 0.57 -0.9 288,570 + GFF256 0.57 -0.7 288,570 + GFF256 0.57 -0.4 288,571 - GFF256 0.57 -1.1 288,621 + GFF256 0.61 -0.2 288,621 + GFF256 0.61 +0.2 288,621 + GFF256 0.61 -1.2 288,621 + GFF256 0.61 -0.5 288,622 - GFF256 0.61 -1.4 288,622 - GFF256 0.61 -0.1 288,651 + GFF256 0.64 +0.6 288,651 + GFF256 0.64 +1.2 288,688 + GFF256 0.67 +0.7 288,688 + GFF256 0.67 -1.2 288,688 + GFF256 0.67 +0.4 288,688 + GFF256 0.67 -0.6 288,689 - GFF256 0.67 -0.9 288,689 - GFF256 0.67 +0.7 288,689 - GFF256 0.67 -0.2 289,065 + +0.9 289,065 + +0.1 290,499 + GFF258 0.27 +0.2 290,499 + GFF258 0.27 -0.7 290,499 + GFF258 0.27 +0.0 290,500 - GFF258 0.27 -2.2 290,538 + GFF258 0.31 -0.0 290,538 + GFF258 0.31 -0.6 290,538 + GFF258 0.31 +0.2 290,538 + GFF258 0.31 +1.5 290,538 + GFF258 0.31 +0.2 290,538 + GFF258 0.31 -0.7 290,538 + GFF258 0.31 +0.3 290,538 + GFF258 0.31 -0.4 290,538 + GFF258 0.31 +0.1 290,538 + GFF258 0.31 -0.3 290,538 + GFF258 0.31 +0.3 290,539 - GFF258 0.32 -1.0 290,539 - GFF258 0.32 +0.6 290,539 - GFF258 0.32 -1.1 290,539 - GFF258 0.32 +0.1 290,539 - GFF258 0.32 -0.2 290,539 - GFF258 0.32 -0.8 290,608 - GFF258 0.39 +0.5 290,608 - GFF258 0.39 +0.4 290,688 + GFF258 0.48 +0.3 290,689 - GFF258 0.49 -1.8 290,826 + GFF258 0.64 -0.1 290,826 + GFF258 0.64 -0.7 290,827 - GFF258 0.64 -0.2 290,853 + GFF258 0.67 -0.1 290,853 + GFF258 0.67 +0.2 290,853 + GFF258 0.67 -1.7 290,853 + GFF258 0.67 +0.2 290,853 + GFF258 0.67 -0.1 290,853 + GFF258 0.67 -0.2 290,853 + GFF258 0.67 -1.0 290,853 + GFF258 0.67 +0.1 290,854 - GFF258 0.67 -1.6 290,854 - GFF258 0.67 -1.6 291,027 + GFF258 0.87 +0.3 291,027 + GFF258 0.87 +0.1 291,027 + GFF258 0.87 -0.5 291,028 - GFF258 0.87 -0.9 291,028 - GFF258 0.87 -0.2 291,028 - GFF258 0.87 -0.5 291,028 - GFF258 0.87 -0.4 291,028 - GFF258 0.87 -0.5 291,028 - GFF258 0.87 +0.1 291,028 - GFF258 0.87 +0.1 291,225 + -0.4 291,225 + -1.7 291,226 - +0.1 291,247 + +0.1 291,248 - +1.7 291,248 - +1.4 291,248 - -0.6 291,248 - +0.8 291,248 - -0.4
Or see this region's nucleotide sequence