Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2547 and GFF2548 are separated by 9 nucleotides GFF2548 and GFF2549 are separated by 37 nucleotides GFF2549 and GFF2550 are separated by 7 nucleotides GFF2550 and GFF2551 overlap by 4 nucleotides
GFF2547 - Type I antifreeze protein, at 610,911 to 611,255
GFF2547
GFF2548 - Uncharacterized membrane anchored protein Mext_4159, at 611,265 to 611,897
GFF2548
GFF2549 - Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23), at 611,935 to 613,740
GFF2549
GFF2550 - Dihydroneopterin triphosphate pyrophosphohydrolase type 2, at 613,748 to 614,224
GFF2550
GFF2551 - Endonuclease/exonuclease/phosphatase, at 614,221 to 614,988
GFF2551
Position (kb) 611
612
613
614 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 611.070 kb on + strand, within GFF2547 at 611.070 kb on + strand, within GFF2547 at 611.070 kb on + strand, within GFF2547 at 611.070 kb on + strand, within GFF2547 at 611.406 kb on + strand, within GFF2548 at 611.553 kb on + strand, within GFF2548 at 611.553 kb on + strand, within GFF2548 at 611.553 kb on + strand, within GFF2548 at 611.553 kb on + strand, within GFF2548 at 611.553 kb on + strand, within GFF2548 at 611.553 kb on + strand, within GFF2548 at 611.727 kb on + strand, within GFF2548 at 611.727 kb on + strand, within GFF2548 at 611.760 kb on + strand, within GFF2548 at 611.760 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.826 kb on + strand, within GFF2548 at 611.830 kb on + strand, within GFF2548 at 614.007 kb on - strand, within GFF2550 at 614.007 kb on - strand, within GFF2550 at 614.015 kb on + strand, within GFF2550 at 614.016 kb on - strand, within GFF2550 at 614.016 kb on - strand, within GFF2550 at 614.148 kb on - strand, within GFF2550 at 614.269 kb on + strand at 614.269 kb on + strand at 614.269 kb on + strand at 614.269 kb on + strand at 614.269 kb on + strand at 614.269 kb on + strand at 614.269 kb on + strand at 614.269 kb on + strand at 614.270 kb on - strand at 614.270 kb on - strand at 614.270 kb on - strand at 614.270 kb on - strand at 614.270 kb on - strand at 614.270 kb on - strand at 614.270 kb on - strand at 614.270 kb on - strand at 614.270 kb on - strand at 614.464 kb on + strand, within GFF2551 at 614.465 kb on - strand, within GFF2551 at 614.465 kb on - strand, within GFF2551 at 614.465 kb on - strand, within GFF2551 at 614.465 kb on - strand, within GFF2551 at 614.465 kb on - strand, within GFF2551 at 614.465 kb on - strand, within GFF2551 at 614.465 kb on - strand, within GFF2551 at 614.465 kb on - strand, within GFF2551 at 614.476 kb on + strand, within GFF2551 at 614.476 kb on + strand, within GFF2551 at 614.477 kb on - strand, within GFF2551
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 611,070 + GFF2547 0.46 -0.2 611,070 + GFF2547 0.46 +0.1 611,070 + GFF2547 0.46 -0.5 611,070 + GFF2547 0.46 -0.7 611,406 + GFF2548 0.22 +0.5 611,553 + GFF2548 0.45 -0.3 611,553 + GFF2548 0.45 -2.8 611,553 + GFF2548 0.45 -0.4 611,553 + GFF2548 0.45 -0.2 611,553 + GFF2548 0.45 -0.4 611,553 + GFF2548 0.45 -0.4 611,727 + GFF2548 0.73 -0.2 611,727 + GFF2548 0.73 -0.0 611,760 + GFF2548 0.78 -0.4 611,760 + GFF2548 0.78 +0.4 611,826 + GFF2548 0.89 -0.9 611,826 + GFF2548 0.89 -0.2 611,826 + GFF2548 0.89 -0.1 611,826 + GFF2548 0.89 +0.6 611,826 + GFF2548 0.89 -0.9 611,826 + GFF2548 0.89 +0.2 611,826 + GFF2548 0.89 -2.3 611,826 + GFF2548 0.89 -2.3 611,826 + GFF2548 0.89 +0.2 611,826 + GFF2548 0.89 +0.3 611,826 + GFF2548 0.89 +0.2 611,826 + GFF2548 0.89 +0.3 611,826 + GFF2548 0.89 -0.6 611,826 + GFF2548 0.89 -0.4 611,826 + GFF2548 0.89 +0.8 611,826 + GFF2548 0.89 -0.5 611,826 + GFF2548 0.89 -0.5 611,830 + GFF2548 0.89 +0.6 614,007 - GFF2550 0.54 -0.9 614,007 - GFF2550 0.54 +0.2 614,015 + GFF2550 0.56 -0.6 614,016 - GFF2550 0.56 -0.5 614,016 - GFF2550 0.56 +0.1 614,148 - GFF2550 0.84 -1.4 614,269 + +0.1 614,269 + -1.4 614,269 + -1.0 614,269 + +0.5 614,269 + -0.5 614,269 + -0.4 614,269 + -0.5 614,269 + +0.4 614,270 - +0.1 614,270 - +0.0 614,270 - -0.0 614,270 - -0.9 614,270 - -0.1 614,270 - -0.5 614,270 - +1.0 614,270 - -0.1 614,270 - -0.6 614,464 + GFF2551 0.32 -0.0 614,465 - GFF2551 0.32 +0.7 614,465 - GFF2551 0.32 +0.4 614,465 - GFF2551 0.32 -1.5 614,465 - GFF2551 0.32 -0.3 614,465 - GFF2551 0.32 +0.4 614,465 - GFF2551 0.32 +0.1 614,465 - GFF2551 0.32 +0.6 614,465 - GFF2551 0.32 +0.2 614,476 + GFF2551 0.33 -1.1 614,476 + GFF2551 0.33 +0.4 614,477 - GFF2551 0.33 -0.5
Or see this region's nucleotide sequence