Strain Fitness in Variovorax sp. SCN45 around GFF2485

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2483 and GFF2484 overlap by 1 nucleotidesGFF2484 and GFF2485 are separated by 13 nucleotidesGFF2485 and GFF2486 are separated by 25 nucleotidesGFF2486 and GFF2487 are separated by 104 nucleotides GFF2483 - Urease alpha subunit (EC 3.5.1.5), at 540,349 to 542,067 GFF2483 GFF2484 - Urease beta subunit (EC 3.5.1.5), at 542,067 to 542,372 GFF2484 GFF2485 - Nickel-binding accessory protein UreJ-HupE, at 542,386 to 542,985 GFF2485 GFF2486 - Urease gamma subunit (EC 3.5.1.5), at 543,011 to 543,313 GFF2486 GFF2487 - Two-component system sensor histidine kinase/response regulator hybrid, at 543,418 to 547,056 GFF2487 Position (kb) 542 543Strain fitness (log2 ratio) -1 0 1at 541.941 kb on - strandat 541.961 kb on + strandat 541.961 kb on + strandat 542.005 kb on + strandat 542.043 kb on - strandat 542.255 kb on - strand, within GFF2484at 542.255 kb on - strand, within GFF2484at 543.219 kb on + strand, within GFF2486at 543.401 kb on + strandat 543.502 kb on + strandat 543.503 kb on - strandat 543.538 kb on + strandat 543.538 kb on + strandat 543.539 kb on - strandat 543.539 kb on - strandat 543.539 kb on - strandat 543.539 kb on - strandat 543.550 kb on + strandat 543.661 kb on + strandat 543.661 kb on + strandat 543.662 kb on - strandat 543.662 kb on - strandat 543.662 kb on - strandat 543.740 kb on - strandat 543.740 kb on - strandat 543.827 kb on - strand, within GFF2487at 543.827 kb on - strand, within GFF2487at 543.827 kb on - strand, within GFF2487at 543.827 kb on - strand, within GFF2487at 543.878 kb on - strand, within GFF2487at 543.919 kb on + strand, within GFF2487at 543.920 kb on - strand, within GFF2487at 543.920 kb on - strand, within GFF2487

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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541,941 - -0.3
541,961 + -1.5
541,961 + -0.1
542,005 + +0.4
542,043 - +0.1
542,255 - GFF2484 0.61 -0.6
542,255 - GFF2484 0.61 -0.2
543,219 + GFF2486 0.69 +0.1
543,401 + +0.1
543,502 + +0.1
543,503 - +0.5
543,538 + -0.1
543,538 + +0.6
543,539 - +0.8
543,539 - +0.2
543,539 - +1.6
543,539 - +0.9
543,550 + -0.5
543,661 + -0.2
543,661 + -0.2
543,662 - +0.8
543,662 - -0.0
543,662 - -0.6
543,740 - +0.3
543,740 - -0.1
543,827 - GFF2487 0.11 +1.2
543,827 - GFF2487 0.11 +0.8
543,827 - GFF2487 0.11 -0.3
543,827 - GFF2487 0.11 +1.4
543,878 - GFF2487 0.13 +0.4
543,919 + GFF2487 0.14 +0.2
543,920 - GFF2487 0.14 -0.4
543,920 - GFF2487 0.14 -0.6

Or see this region's nucleotide sequence