Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2426 and GFF2427 are separated by 160 nucleotides GFF2427 and GFF2428 are separated by 14 nucleotides GFF2428 and GFF2429 are separated by 7 nucleotides GFF2429 and GFF2430 are separated by 22 nucleotides
GFF2426 - ABC transporter, substrate-binding protein (cluster 1, maltose/g3p/polyamine/iron), at 477,016 to 478,050
GFF2426
GFF2427 - Transcriptional regulator, MarR family, at 478,211 to 478,834
GFF2427
GFF2428 - Transcriptional regulator, LysR family, at 478,849 to 479,763
GFF2428
GFF2429 - putative membrane protein, at 479,771 to 480,595
GFF2429
GFF2430 - Protein secretion chaperonin CsaA, at 480,618 to 480,977
GFF2430
Position (kb) 478
479
480 Strain fitness (log2 ratio) -2
-1
0
1 at 478.133 kb on - strand at 478.275 kb on + strand, within GFF2427 at 478.451 kb on + strand, within GFF2427 at 478.451 kb on + strand, within GFF2427 at 478.452 kb on - strand, within GFF2427 at 478.452 kb on - strand, within GFF2427 at 478.873 kb on + strand at 478.874 kb on - strand at 478.874 kb on - strand at 479.818 kb on + strand at 479.819 kb on - strand at 479.907 kb on - strand, within GFF2429 at 480.182 kb on + strand, within GFF2429 at 480.182 kb on + strand, within GFF2429 at 480.183 kb on - strand, within GFF2429 at 480.243 kb on + strand, within GFF2429 at 480.302 kb on + strand, within GFF2429 at 480.575 kb on + strand at 480.576 kb on - strand at 480.642 kb on + strand at 480.642 kb on + strand at 480.642 kb on + strand at 480.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 478,133 - -1.7 478,275 + GFF2427 0.10 +0.0 478,451 + GFF2427 0.38 -0.3 478,451 + GFF2427 0.38 -0.8 478,452 - GFF2427 0.39 +0.0 478,452 - GFF2427 0.39 +0.1 478,873 + +0.1 478,874 - -0.1 478,874 - +0.8 479,818 + +1.1 479,819 - +0.9 479,907 - GFF2429 0.16 -0.8 480,182 + GFF2429 0.50 -0.3 480,182 + GFF2429 0.50 +1.4 480,183 - GFF2429 0.50 -1.9 480,243 + GFF2429 0.57 -0.2 480,302 + GFF2429 0.64 +0.2 480,575 + -1.0 480,576 - +0.1 480,642 + -0.3 480,642 + -2.4 480,642 + +0.3 480,643 - -0.1
Or see this region's nucleotide sequence