Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF229 and GFF230 are separated by 37 nucleotides GFF230 and GFF231 are separated by 62 nucleotides GFF231 and GFF232 are separated by 45 nucleotides
GFF229 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 256,748 to 258,562
GFF229
GFF230 - Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9), at 258,600 to 259,382
GFF230
GFF231 - DNA ligase (ATP) (EC 6.5.1.1), at 259,445 to 260,287
GFF231
GFF232 - Arginine decarboxylase (EC 4.1.1.19); Ornithine decarboxylase (EC 4.1.1.17); Lysine decarboxylase (EC 4.1.1.18), at 260,333 to 262,639
GFF232
Position (kb)
258
259
260 Strain fitness (log2 ratio)
-2
-1
0
1 at 257.727 kb on + strand, within GFF229 at 257.727 kb on + strand, within GFF229 at 257.728 kb on - strand, within GFF229 at 257.728 kb on - strand, within GFF229 at 257.728 kb on - strand, within GFF229 at 257.790 kb on + strand, within GFF229 at 257.790 kb on + strand, within GFF229 at 257.790 kb on + strand, within GFF229 at 257.791 kb on - strand, within GFF229 at 257.791 kb on - strand, within GFF229 at 257.791 kb on - strand, within GFF229 at 257.791 kb on - strand, within GFF229 at 257.856 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.914 kb on - strand, within GFF229 at 257.914 kb on - strand, within GFF229 at 257.914 kb on - strand, within GFF229 at 257.914 kb on - strand, within GFF229 at 258.114 kb on + strand, within GFF229 at 258.114 kb on + strand, within GFF229 at 258.115 kb on - strand, within GFF229 at 258.115 kb on - strand, within GFF229 at 258.115 kb on - strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.130 kb on - strand, within GFF229 at 258.150 kb on + strand, within GFF229 at 258.150 kb on + strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.447 kb on + strand at 258.447 kb on + strand at 258.447 kb on + strand at 258.474 kb on + strand at 258.474 kb on + strand at 258.475 kb on - strand at 258.498 kb on + strand at 258.498 kb on + strand at 258.498 kb on + strand at 258.498 kb on + strand at 258.498 kb on + strand at 258.499 kb on - strand at 258.499 kb on - strand at 258.499 kb on - strand at 258.499 kb on - strand at 258.501 kb on - strand at 258.641 kb on - strand at 258.641 kb on - strand at 258.664 kb on - strand at 259.036 kb on - strand, within GFF230 at 259.335 kb on + strand at 259.381 kb on - strand at 259.427 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.432 kb on - strand at 259.432 kb on - strand at 259.432 kb on - strand at 259.432 kb on - strand at 259.438 kb on + strand at 259.438 kb on + strand at 259.438 kb on + strand at 259.438 kb on + strand at 259.439 kb on - strand at 259.439 kb on - strand at 259.439 kb on - strand at 259.439 kb on - strand at 259.547 kb on + strand, within GFF231 at 259.548 kb on - strand, within GFF231 at 259.548 kb on - strand, within GFF231 at 259.592 kb on + strand, within GFF231 at 259.593 kb on - strand, within GFF231 at 259.593 kb on - strand, within GFF231 at 259.611 kb on - strand, within GFF231 at 259.611 kb on - strand, within GFF231 at 259.611 kb on - strand, within GFF231 at 259.611 kb on - strand, within GFF231 at 260.216 kb on + strand at 260.216 kb on + strand at 260.216 kb on + strand at 260.217 kb on - strand at 260.217 kb on - strand at 260.334 kb on + strand at 260.334 kb on + strand at 260.334 kb on + strand at 260.334 kb on + strand at 260.334 kb on + strand at 260.334 kb on + strand at 260.334 kb on + strand at 260.335 kb on - strand at 260.335 kb on - strand at 260.335 kb on - strand at 260.364 kb on + strand at 260.364 kb on + strand at 260.365 kb on - strand at 260.365 kb on - strand at 260.365 kb on - strand at 260.365 kb on - strand at 260.365 kb on - strand at 260.365 kb on - strand at 260.367 kb on + strand at 260.368 kb on - strand at 260.368 kb on - strand at 260.369 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 257,727 + GFF229 0.54 -0.8 257,727 + GFF229 0.54 +0.0 257,728 - GFF229 0.54 -0.2 257,728 - GFF229 0.54 +0.2 257,728 - GFF229 0.54 -1.7 257,790 + GFF229 0.57 -1.2 257,790 + GFF229 0.57 -0.1 257,790 + GFF229 0.57 -0.7 257,791 - GFF229 0.57 -1.4 257,791 - GFF229 0.57 -1.1 257,791 - GFF229 0.57 -2.0 257,791 - GFF229 0.57 +0.0 257,856 + GFF229 0.61 -0.6 257,874 + GFF229 0.62 -0.9 257,874 + GFF229 0.62 -0.4 257,874 + GFF229 0.62 -0.2 257,874 + GFF229 0.62 -0.1 257,874 + GFF229 0.62 -0.0 257,875 - GFF229 0.62 +0.2 257,875 - GFF229 0.62 +0.0 257,875 - GFF229 0.62 -2.5 257,875 - GFF229 0.62 -0.2 257,875 - GFF229 0.62 +0.7 257,875 - GFF229 0.62 +0.1 257,875 - GFF229 0.62 +0.2 257,875 - GFF229 0.62 -0.9 257,875 - GFF229 0.62 +0.3 257,913 + GFF229 0.64 -2.4 257,913 + GFF229 0.64 -0.3 257,913 + GFF229 0.64 +1.6 257,913 + GFF229 0.64 -0.6 257,913 + GFF229 0.64 -0.7 257,913 + GFF229 0.64 -0.4 257,913 + GFF229 0.64 -1.5 257,914 - GFF229 0.64 -0.4 257,914 - GFF229 0.64 -1.5 257,914 - GFF229 0.64 -0.0 257,914 - GFF229 0.64 -0.5 258,114 + GFF229 0.75 -0.9 258,114 + GFF229 0.75 +0.8 258,115 - GFF229 0.75 -0.5 258,115 - GFF229 0.75 -0.8 258,115 - GFF229 0.75 -0.7 258,129 + GFF229 0.76 -1.1 258,129 + GFF229 0.76 -0.6 258,129 + GFF229 0.76 -0.8 258,129 + GFF229 0.76 +0.8 258,129 + GFF229 0.76 +0.2 258,130 - GFF229 0.76 -1.2 258,150 + GFF229 0.77 -0.6 258,150 + GFF229 0.77 -1.3 258,151 - GFF229 0.77 +0.2 258,151 - GFF229 0.77 -1.0 258,151 - GFF229 0.77 +0.0 258,151 - GFF229 0.77 +0.6 258,151 - GFF229 0.77 -0.0 258,151 - GFF229 0.77 -0.5 258,372 + GFF229 0.89 -0.2 258,372 + GFF229 0.89 -0.5 258,372 + GFF229 0.89 -0.2 258,372 + GFF229 0.89 +0.1 258,372 + GFF229 0.89 -0.7 258,372 + GFF229 0.89 -0.6 258,372 + GFF229 0.89 -0.7 258,372 + GFF229 0.89 -1.5 258,373 - GFF229 0.90 -1.5 258,373 - GFF229 0.90 -0.3 258,373 - GFF229 0.90 -0.4 258,373 - GFF229 0.90 -1.4 258,373 - GFF229 0.90 +1.2 258,373 - GFF229 0.90 -0.4 258,447 + +0.0 258,447 + -0.0 258,447 + +0.6 258,474 + +0.0 258,474 + -1.2 258,475 - -1.1 258,498 + -0.7 258,498 + -2.1 258,498 + -2.0 258,498 + -0.8 258,498 + -0.8 258,499 - -0.9 258,499 - +1.2 258,499 - -0.2 258,499 - -0.8 258,501 - +0.2 258,641 - -1.4 258,641 - -0.8 258,664 - -1.6 259,036 - GFF230 0.56 -1.8 259,335 + -0.8 259,381 - +1.7 259,427 + +0.2 259,431 + -0.4 259,431 + +1.2 259,431 + -0.6 259,431 + -0.0 259,431 + +0.3 259,431 + +0.6 259,431 + -2.0 259,432 - +0.1 259,432 - +0.3 259,432 - -0.4 259,432 - -0.3 259,438 + +0.5 259,438 + -0.3 259,438 + -0.7 259,438 + -0.1 259,439 - -0.4 259,439 - -0.1 259,439 - +0.1 259,439 - -0.7 259,547 + GFF231 0.12 -1.2 259,548 - GFF231 0.12 -0.5 259,548 - GFF231 0.12 -0.6 259,592 + GFF231 0.17 -0.5 259,593 - GFF231 0.18 -1.1 259,593 - GFF231 0.18 -0.4 259,611 - GFF231 0.20 +0.1 259,611 - GFF231 0.20 +0.2 259,611 - GFF231 0.20 -0.3 259,611 - GFF231 0.20 +0.5 260,216 + +0.4 260,216 + -0.7 260,216 + -1.2 260,217 - +1.7 260,217 - -1.1 260,334 + -0.1 260,334 + -1.2 260,334 + -0.5 260,334 + -0.4 260,334 + -0.3 260,334 + -0.3 260,334 + -0.4 260,335 - +0.1 260,335 - -0.6 260,335 - +0.2 260,364 + -1.8 260,364 + +0.8 260,365 - -0.3 260,365 - +1.4 260,365 - +0.4 260,365 - +0.5 260,365 - +0.1 260,365 - +0.1 260,367 + -2.1 260,368 - -0.2 260,368 - -0.8 260,369 - -1.8
Or see this region's nucleotide sequence