Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2209 and GFF2210 are separated by 40 nucleotides GFF2210 and GFF2211 are separated by 39 nucleotides
GFF2209 - hypothetical protein, at 270,845 to 272,128
GFF2209
GFF2210 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 272,169 to 273,560
GFF2210
GFF2211 - Flp pilus assembly protein RcpC/CpaB, at 273,600 to 274,595
GFF2211
Position (kb) 272
273
274 Strain fitness (log2 ratio) -2
-1
0
1
2 at 271.524 kb on + strand, within GFF2209 at 272.083 kb on - strand at 272.083 kb on - strand at 272.083 kb on - strand at 272.218 kb on + strand at 272.218 kb on + strand at 272.218 kb on + strand at 272.218 kb on + strand at 272.350 kb on + strand, within GFF2210 at 272.350 kb on + strand, within GFF2210 at 272.504 kb on + strand, within GFF2210 at 272.548 kb on + strand, within GFF2210 at 272.549 kb on - strand, within GFF2210 at 272.549 kb on - strand, within GFF2210 at 272.644 kb on + strand, within GFF2210 at 272.644 kb on + strand, within GFF2210 at 272.644 kb on + strand, within GFF2210 at 272.645 kb on - strand, within GFF2210 at 272.751 kb on + strand, within GFF2210 at 272.772 kb on - strand, within GFF2210 at 272.772 kb on - strand, within GFF2210 at 272.799 kb on + strand, within GFF2210 at 272.800 kb on - strand, within GFF2210 at 272.898 kb on + strand, within GFF2210 at 272.898 kb on + strand, within GFF2210 at 272.986 kb on - strand, within GFF2210 at 272.986 kb on - strand, within GFF2210 at 272.986 kb on - strand, within GFF2210 at 272.986 kb on - strand, within GFF2210 at 273.109 kb on - strand, within GFF2210 at 273.178 kb on + strand, within GFF2210 at 273.178 kb on + strand, within GFF2210 at 273.178 kb on + strand, within GFF2210 at 273.179 kb on - strand, within GFF2210 at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.553 kb on + strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.554 kb on - strand at 273.555 kb on - strand at 273.604 kb on + strand at 273.604 kb on + strand at 273.604 kb on + strand at 273.604 kb on + strand at 273.604 kb on + strand at 273.604 kb on + strand at 273.605 kb on - strand at 273.605 kb on - strand at 273.605 kb on - strand at 273.605 kb on - strand at 273.605 kb on - strand at 273.605 kb on - strand at 273.608 kb on + strand at 273.608 kb on + strand at 273.609 kb on - strand at 273.635 kb on + strand at 273.636 kb on - strand at 274.441 kb on + strand, within GFF2211 at 274.454 kb on - strand, within GFF2211 at 274.531 kb on + strand at 274.531 kb on + strand at 274.531 kb on + strand at 274.532 kb on - strand at 274.532 kb on - strand at 274.532 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 271,524 + GFF2209 0.53 -0.3 272,083 - -0.4 272,083 - -0.2 272,083 - +0.4 272,218 + +0.3 272,218 + -0.2 272,218 + -0.6 272,218 + -0.2 272,350 + GFF2210 0.13 -0.5 272,350 + GFF2210 0.13 -0.5 272,504 + GFF2210 0.24 -0.6 272,548 + GFF2210 0.27 -0.2 272,549 - GFF2210 0.27 -0.8 272,549 - GFF2210 0.27 -0.3 272,644 + GFF2210 0.34 -0.7 272,644 + GFF2210 0.34 -0.2 272,644 + GFF2210 0.34 +0.1 272,645 - GFF2210 0.34 -1.5 272,751 + GFF2210 0.42 +0.7 272,772 - GFF2210 0.43 -0.9 272,772 - GFF2210 0.43 +0.2 272,799 + GFF2210 0.45 -1.8 272,800 - GFF2210 0.45 +0.4 272,898 + GFF2210 0.52 -0.6 272,898 + GFF2210 0.52 -1.2 272,986 - GFF2210 0.59 -1.7 272,986 - GFF2210 0.59 +0.0 272,986 - GFF2210 0.59 +0.8 272,986 - GFF2210 0.59 -1.3 273,109 - GFF2210 0.68 +1.0 273,178 + GFF2210 0.72 -0.8 273,178 + GFF2210 0.72 -1.2 273,178 + GFF2210 0.72 -1.0 273,179 - GFF2210 0.73 -0.4 273,553 + -0.5 273,553 + +1.5 273,553 + +0.4 273,553 + -1.1 273,553 + +0.1 273,553 + -1.4 273,553 + +0.6 273,553 + -0.3 273,553 + -1.6 273,553 + -0.1 273,553 + +0.3 273,553 + -0.5 273,553 + +0.7 273,553 + +0.8 273,553 + -0.1 273,553 + -0.6 273,553 + +0.1 273,553 + -0.3 273,553 + +0.2 273,554 - -0.2 273,554 - +0.0 273,554 - -1.5 273,554 - -0.7 273,554 - +1.1 273,554 - -0.4 273,554 - +0.1 273,554 - +0.2 273,554 - -0.3 273,554 - +0.1 273,554 - -0.3 273,554 - -0.5 273,554 - -0.7 273,554 - +0.1 273,554 - -0.4 273,554 - -0.8 273,554 - -0.6 273,554 - +0.0 273,555 - -0.0 273,604 + +2.0 273,604 + +0.2 273,604 + +0.0 273,604 + -1.7 273,604 + +0.4 273,604 + +0.4 273,605 - +0.4 273,605 - -0.8 273,605 - +0.6 273,605 - +0.4 273,605 - -0.4 273,605 - +0.4 273,608 + -0.7 273,608 + -0.5 273,609 - +1.4 273,635 + +0.7 273,636 - +0.1 274,441 + GFF2211 0.84 +0.3 274,454 - GFF2211 0.86 -0.8 274,531 + -0.5 274,531 + +0.1 274,531 + +2.0 274,532 - +0.5 274,532 - -0.0 274,532 - +0.3
Or see this region's nucleotide sequence