Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2144 and GFF2145 are separated by 16 nucleotides GFF2145 and GFF2146 are separated by 16 nucleotides GFF2146 and GFF2147 are separated by 90 nucleotides GFF2147 and GFF2148 are separated by 3 nucleotides
GFF2144 - tRNA-dihydrouridine synthase DusB, at 193,146 to 194,207
GFF2144
GFF2145 - DNA-binding protein Fis, at 194,224 to 194,460
GFF2145
GFF2146 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 194,477 to 196,084
GFF2146
GFF2147 - no description, at 196,175 to 196,690
GFF2147
GFF2148 - Glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8), at 196,694 to 197,974
GFF2148
Position (kb) 194
195
196
197 Strain fitness (log2 ratio) -2
-1
0
1
2 at 193.689 kb on + strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.959 kb on + strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.969 kb on - strand, within GFF2144 at 193.977 kb on + strand, within GFF2144 at 193.978 kb on - strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.017 kb on - strand, within GFF2144 at 194.017 kb on - strand, within GFF2144 at 194.017 kb on - strand, within GFF2144 at 194.272 kb on + strand, within GFF2145 at 194.458 kb on + strand at 196.157 kb on - strand at 196.176 kb on + strand at 196.176 kb on + strand at 196.197 kb on + strand at 196.197 kb on + strand at 196.251 kb on + strand, within GFF2147 at 196.251 kb on + strand, within GFF2147 at 196.252 kb on - strand, within GFF2147 at 196.252 kb on - strand, within GFF2147 at 196.329 kb on + strand, within GFF2147 at 196.330 kb on - strand, within GFF2147 at 196.330 kb on - strand, within GFF2147 at 196.339 kb on - strand, within GFF2147 at 196.380 kb on + strand, within GFF2147 at 196.381 kb on - strand, within GFF2147 at 196.381 kb on - strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.397 kb on + strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.398 kb on - strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.401 kb on + strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.402 kb on - strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.413 kb on + strand, within GFF2147 at 196.414 kb on - strand, within GFF2147 at 196.464 kb on + strand, within GFF2147 at 196.464 kb on + strand, within GFF2147 at 196.464 kb on + strand, within GFF2147 at 196.465 kb on - strand, within GFF2147 at 196.465 kb on - strand, within GFF2147 at 196.465 kb on - strand, within GFF2147 at 196.482 kb on + strand, within GFF2147 at 196.545 kb on + strand, within GFF2147 at 196.546 kb on - strand, within GFF2147 at 196.695 kb on + strand at 196.695 kb on + strand at 196.695 kb on + strand at 196.695 kb on + strand at 196.695 kb on + strand at 196.696 kb on - strand at 196.706 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 193,689 + GFF2144 0.51 +0.3 193,689 + GFF2144 0.51 +0.4 193,689 + GFF2144 0.51 -0.4 193,689 + GFF2144 0.51 -0.2 193,689 + GFF2144 0.51 -0.4 193,690 - GFF2144 0.51 +0.6 193,690 - GFF2144 0.51 -0.6 193,690 - GFF2144 0.51 +1.2 193,690 - GFF2144 0.51 -0.3 193,690 - GFF2144 0.51 +0.6 193,690 - GFF2144 0.51 +0.1 193,959 + GFF2144 0.77 +0.1 193,960 - GFF2144 0.77 +0.9 193,960 - GFF2144 0.77 +1.1 193,960 - GFF2144 0.77 -1.6 193,960 - GFF2144 0.77 -0.3 193,960 - GFF2144 0.77 -0.1 193,960 - GFF2144 0.77 -0.8 193,969 - GFF2144 0.77 +0.7 193,977 + GFF2144 0.78 +0.1 193,978 - GFF2144 0.78 +0.9 194,016 + GFF2144 0.82 -0.7 194,016 + GFF2144 0.82 +1.4 194,016 + GFF2144 0.82 -0.0 194,016 + GFF2144 0.82 -0.3 194,016 + GFF2144 0.82 +0.1 194,016 + GFF2144 0.82 -0.1 194,017 - GFF2144 0.82 +0.2 194,017 - GFF2144 0.82 -0.0 194,017 - GFF2144 0.82 +0.9 194,272 + GFF2145 0.20 -1.1 194,458 + +0.7 196,157 - +0.7 196,176 + +0.3 196,176 + -0.2 196,197 + -0.1 196,197 + +0.5 196,251 + GFF2147 0.15 -0.3 196,251 + GFF2147 0.15 -0.3 196,252 - GFF2147 0.15 +0.2 196,252 - GFF2147 0.15 -0.6 196,329 + GFF2147 0.30 -0.5 196,330 - GFF2147 0.30 -0.1 196,330 - GFF2147 0.30 +0.3 196,339 - GFF2147 0.32 -2.3 196,380 + GFF2147 0.40 -0.5 196,381 - GFF2147 0.40 +0.1 196,381 - GFF2147 0.40 +0.2 196,397 + GFF2147 0.43 +0.0 196,397 + GFF2147 0.43 -0.3 196,397 + GFF2147 0.43 -0.3 196,397 + GFF2147 0.43 -0.3 196,397 + GFF2147 0.43 +1.2 196,397 + GFF2147 0.43 -0.6 196,397 + GFF2147 0.43 +0.5 196,397 + GFF2147 0.43 -0.3 196,398 - GFF2147 0.43 +0.2 196,398 - GFF2147 0.43 -0.1 196,398 - GFF2147 0.43 +0.4 196,398 - GFF2147 0.43 -1.0 196,398 - GFF2147 0.43 -0.3 196,398 - GFF2147 0.43 +0.2 196,401 + GFF2147 0.44 +0.6 196,401 + GFF2147 0.44 -0.3 196,401 + GFF2147 0.44 -1.1 196,401 + GFF2147 0.44 +0.4 196,401 + GFF2147 0.44 -0.6 196,401 + GFF2147 0.44 +0.2 196,401 + GFF2147 0.44 +0.9 196,401 + GFF2147 0.44 -2.2 196,401 + GFF2147 0.44 -2.6 196,401 + GFF2147 0.44 -0.4 196,401 + GFF2147 0.44 +0.7 196,401 + GFF2147 0.44 +0.1 196,401 + GFF2147 0.44 +2.7 196,402 - GFF2147 0.44 -0.3 196,402 - GFF2147 0.44 -0.4 196,402 - GFF2147 0.44 +1.3 196,402 - GFF2147 0.44 -1.8 196,402 - GFF2147 0.44 +1.0 196,402 - GFF2147 0.44 -0.3 196,402 - GFF2147 0.44 -0.2 196,402 - GFF2147 0.44 +0.5 196,402 - GFF2147 0.44 -0.8 196,402 - GFF2147 0.44 -0.1 196,402 - GFF2147 0.44 +0.1 196,402 - GFF2147 0.44 -1.2 196,402 - GFF2147 0.44 -0.6 196,402 - GFF2147 0.44 -0.4 196,402 - GFF2147 0.44 +0.2 196,413 + GFF2147 0.46 -0.8 196,413 + GFF2147 0.46 +0.3 196,413 + GFF2147 0.46 -0.7 196,413 + GFF2147 0.46 -0.3 196,413 + GFF2147 0.46 -1.5 196,414 - GFF2147 0.46 -1.0 196,464 + GFF2147 0.56 -0.1 196,464 + GFF2147 0.56 -1.2 196,464 + GFF2147 0.56 -0.3 196,465 - GFF2147 0.56 +0.1 196,465 - GFF2147 0.56 -0.9 196,465 - GFF2147 0.56 -0.9 196,482 + GFF2147 0.59 +0.6 196,545 + GFF2147 0.72 -0.4 196,546 - GFF2147 0.72 -0.2 196,695 + -0.3 196,695 + +0.5 196,695 + -0.3 196,695 + +1.5 196,695 + -0.1 196,696 - +0.9 196,706 + +1.0
Or see this region's nucleotide sequence