Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2142 and GFF2143 are separated by 21 nucleotides GFF2143 and GFF2144 are separated by 172 nucleotides GFF2144 and GFF2145 are separated by 16 nucleotides GFF2145 and GFF2146 are separated by 16 nucleotides
GFF2142 - Glutathione S-transferase (EC 2.5.1.18), at 191,813 to 192,487
GFF2142
GFF2143 - probable membrane protein YPO3302, at 192,509 to 192,973
GFF2143
GFF2144 - tRNA-dihydrouridine synthase DusB, at 193,146 to 194,207
GFF2144
GFF2145 - DNA-binding protein Fis, at 194,224 to 194,460
GFF2145
GFF2146 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 194,477 to 196,084
GFF2146
Position (kb)
193
194
195 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 192.410 kb on + strand, within GFF2142 at 192.411 kb on - strand, within GFF2142 at 192.440 kb on + strand at 192.441 kb on - strand at 192.441 kb on - strand at 192.441 kb on - strand at 192.441 kb on - strand at 192.546 kb on + strand at 192.546 kb on + strand at 192.547 kb on - strand at 192.573 kb on + strand, within GFF2143 at 192.573 kb on + strand, within GFF2143 at 192.573 kb on + strand, within GFF2143 at 192.573 kb on + strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.723 kb on + strand, within GFF2143 at 192.724 kb on - strand, within GFF2143 at 192.724 kb on - strand, within GFF2143 at 192.724 kb on - strand, within GFF2143 at 192.751 kb on - strand, within GFF2143 at 192.924 kb on + strand, within GFF2143 at 192.924 kb on + strand, within GFF2143 at 192.925 kb on - strand, within GFF2143 at 192.973 kb on + strand at 192.973 kb on + strand at 192.973 kb on + strand at 192.974 kb on - strand at 192.974 kb on - strand at 192.974 kb on - strand at 192.986 kb on + strand at 192.986 kb on + strand at 192.986 kb on + strand at 192.986 kb on + strand at 192.986 kb on + strand at 192.987 kb on - strand at 192.987 kb on - strand at 192.987 kb on - strand at 192.987 kb on - strand at 192.988 kb on + strand at 192.988 kb on + strand at 192.988 kb on + strand at 192.988 kb on + strand at 192.989 kb on - strand at 192.989 kb on - strand at 192.989 kb on - strand at 193.044 kb on + strand at 193.044 kb on + strand at 193.044 kb on + strand at 193.404 kb on + strand, within GFF2144 at 193.404 kb on + strand, within GFF2144 at 193.404 kb on + strand, within GFF2144 at 193.405 kb on - strand, within GFF2144 at 193.405 kb on - strand, within GFF2144 at 193.405 kb on - strand, within GFF2144 at 193.405 kb on - strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.689 kb on + strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.690 kb on - strand, within GFF2144 at 193.959 kb on + strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.960 kb on - strand, within GFF2144 at 193.969 kb on - strand, within GFF2144 at 193.977 kb on + strand, within GFF2144 at 193.978 kb on - strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.016 kb on + strand, within GFF2144 at 194.017 kb on - strand, within GFF2144 at 194.017 kb on - strand, within GFF2144 at 194.017 kb on - strand, within GFF2144 at 194.272 kb on + strand, within GFF2145 at 194.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 192,410 + GFF2142 0.88 +1.0 192,411 - GFF2142 0.89 +1.7 192,440 + -1.0 192,441 - -0.0 192,441 - +1.4 192,441 - +0.4 192,441 - -0.6 192,546 + +0.0 192,546 + +0.1 192,547 - +0.2 192,573 + GFF2143 0.14 -0.0 192,573 + GFF2143 0.14 -0.9 192,573 + GFF2143 0.14 -1.2 192,573 + GFF2143 0.14 +0.5 192,574 - GFF2143 0.14 +0.3 192,574 - GFF2143 0.14 -0.8 192,574 - GFF2143 0.14 +1.7 192,574 - GFF2143 0.14 -0.2 192,574 - GFF2143 0.14 +0.5 192,574 - GFF2143 0.14 -0.6 192,574 - GFF2143 0.14 -1.1 192,574 - GFF2143 0.14 -0.0 192,574 - GFF2143 0.14 +0.9 192,723 + GFF2143 0.46 -0.5 192,724 - GFF2143 0.46 +0.8 192,724 - GFF2143 0.46 -0.1 192,724 - GFF2143 0.46 +1.7 192,751 - GFF2143 0.52 +1.0 192,924 + GFF2143 0.89 -0.8 192,924 + GFF2143 0.89 -1.2 192,925 - GFF2143 0.89 +0.2 192,973 + -0.2 192,973 + -1.0 192,973 + -1.0 192,974 - +0.4 192,974 - +0.0 192,974 - -0.1 192,986 + +2.2 192,986 + -0.1 192,986 + -1.5 192,986 + -0.5 192,986 + +0.1 192,987 - +0.3 192,987 - -0.9 192,987 - -0.4 192,987 - -0.4 192,988 + -0.1 192,988 + +1.7 192,988 + -0.5 192,988 + -1.3 192,989 - +0.5 192,989 - -0.6 192,989 - -0.2 193,044 + -0.6 193,044 + -0.9 193,044 + +0.6 193,404 + GFF2144 0.24 +0.9 193,404 + GFF2144 0.24 -0.1 193,404 + GFF2144 0.24 -1.3 193,405 - GFF2144 0.24 -1.8 193,405 - GFF2144 0.24 -0.6 193,405 - GFF2144 0.24 +0.6 193,405 - GFF2144 0.24 +1.2 193,689 + GFF2144 0.51 +0.3 193,689 + GFF2144 0.51 +0.4 193,689 + GFF2144 0.51 -0.4 193,689 + GFF2144 0.51 -0.2 193,689 + GFF2144 0.51 -0.4 193,690 - GFF2144 0.51 +0.6 193,690 - GFF2144 0.51 -0.6 193,690 - GFF2144 0.51 +1.2 193,690 - GFF2144 0.51 -0.3 193,690 - GFF2144 0.51 +0.6 193,690 - GFF2144 0.51 +0.1 193,959 + GFF2144 0.77 +0.1 193,960 - GFF2144 0.77 +0.9 193,960 - GFF2144 0.77 +1.1 193,960 - GFF2144 0.77 -1.6 193,960 - GFF2144 0.77 -0.3 193,960 - GFF2144 0.77 -0.1 193,960 - GFF2144 0.77 -0.8 193,969 - GFF2144 0.77 +0.7 193,977 + GFF2144 0.78 +0.1 193,978 - GFF2144 0.78 +0.9 194,016 + GFF2144 0.82 -0.7 194,016 + GFF2144 0.82 +1.4 194,016 + GFF2144 0.82 -0.0 194,016 + GFF2144 0.82 -0.3 194,016 + GFF2144 0.82 +0.1 194,016 + GFF2144 0.82 -0.1 194,017 - GFF2144 0.82 +0.2 194,017 - GFF2144 0.82 -0.0 194,017 - GFF2144 0.82 +0.9 194,272 + GFF2145 0.20 -1.1 194,458 + +0.7
Or see this region's nucleotide sequence