Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2131 and GFF2132 overlap by 1 nucleotides GFF2132 and GFF2133 are separated by 40 nucleotides GFF2133 and GFF2134 are separated by 87 nucleotides GFF2134 and GFF2135 are separated by 22 nucleotides
GFF2131 - Putative protease, at 179,503 to 181,353
GFF2131
GFF2132 - Thiol:disulfide interchange protein DsbC, at 181,353 to 182,075
GFF2132
GFF2133 - 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 182,116 to 183,243
GFF2133
GFF2134 - probable iron-sulfur binding protein YPO1417, at 183,331 to 184,212
GFF2134
GFF2135 - D-ribose pyranase (EC 5.4.99.62), at 184,235 to 184,627
GFF2135
Position (kb) 182
183
184 Strain fitness (log2 ratio) -1
0
1 at 181.175 kb on + strand at 181.175 kb on + strand at 181.176 kb on - strand at 181.176 kb on - strand at 181.459 kb on + strand, within GFF2132 at 181.459 kb on + strand, within GFF2132 at 181.459 kb on + strand, within GFF2132 at 181.459 kb on + strand, within GFF2132 at 181.459 kb on + strand, within GFF2132 at 181.459 kb on + strand, within GFF2132 at 181.460 kb on - strand, within GFF2132 at 181.460 kb on - strand, within GFF2132 at 181.460 kb on - strand, within GFF2132 at 181.460 kb on - strand, within GFF2132 at 181.628 kb on - strand, within GFF2132 at 181.639 kb on + strand, within GFF2132 at 181.639 kb on + strand, within GFF2132 at 181.639 kb on + strand, within GFF2132 at 181.639 kb on + strand, within GFF2132 at 181.640 kb on - strand, within GFF2132 at 181.640 kb on - strand, within GFF2132 at 181.640 kb on - strand, within GFF2132 at 181.640 kb on - strand, within GFF2132 at 181.640 kb on - strand, within GFF2132 at 181.690 kb on + strand, within GFF2132 at 181.690 kb on + strand, within GFF2132 at 181.691 kb on - strand, within GFF2132 at 181.864 kb on + strand, within GFF2132 at 181.864 kb on + strand, within GFF2132 at 181.864 kb on + strand, within GFF2132 at 181.864 kb on + strand, within GFF2132 at 181.864 kb on + strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.865 kb on - strand, within GFF2132 at 181.885 kb on + strand, within GFF2132 at 181.885 kb on + strand, within GFF2132 at 181.885 kb on + strand, within GFF2132 at 181.886 kb on - strand, within GFF2132 at 181.886 kb on - strand, within GFF2132 at 181.886 kb on - strand, within GFF2132 at 181.886 kb on - strand, within GFF2132 at 181.919 kb on - strand, within GFF2132 at 182.052 kb on + strand at 182.053 kb on - strand at 183.254 kb on + strand at 183.254 kb on + strand at 183.254 kb on + strand at 183.254 kb on + strand at 183.254 kb on + strand at 183.254 kb on + strand at 183.254 kb on + strand at 183.255 kb on - strand at 183.255 kb on - strand at 183.255 kb on - strand at 183.255 kb on - strand at 183.255 kb on - strand at 183.255 kb on - strand at 183.255 kb on - strand at 183.261 kb on + strand at 183.261 kb on + strand at 183.261 kb on + strand at 183.261 kb on + strand at 183.262 kb on - strand at 183.262 kb on - strand at 183.262 kb on - strand at 183.262 kb on - strand at 183.383 kb on - strand at 183.649 kb on + strand, within GFF2134 at 183.812 kb on - strand, within GFF2134 at 184.090 kb on + strand, within GFF2134 at 184.198 kb on + strand at 184.198 kb on + strand at 184.198 kb on + strand at 184.199 kb on - strand at 184.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 181,175 + -0.4 181,175 + -0.1 181,176 - -0.7 181,176 - -0.1 181,459 + GFF2132 0.15 -0.2 181,459 + GFF2132 0.15 +1.0 181,459 + GFF2132 0.15 +0.3 181,459 + GFF2132 0.15 -0.5 181,459 + GFF2132 0.15 +1.0 181,459 + GFF2132 0.15 -0.3 181,460 - GFF2132 0.15 -0.3 181,460 - GFF2132 0.15 +1.1 181,460 - GFF2132 0.15 -0.4 181,460 - GFF2132 0.15 +0.8 181,628 - GFF2132 0.38 +0.7 181,639 + GFF2132 0.40 -0.2 181,639 + GFF2132 0.40 +1.7 181,639 + GFF2132 0.40 +0.3 181,639 + GFF2132 0.40 -0.8 181,640 - GFF2132 0.40 -0.1 181,640 - GFF2132 0.40 -0.1 181,640 - GFF2132 0.40 -0.5 181,640 - GFF2132 0.40 -1.4 181,640 - GFF2132 0.40 -0.4 181,690 + GFF2132 0.47 +0.1 181,690 + GFF2132 0.47 -0.0 181,691 - GFF2132 0.47 -1.0 181,864 + GFF2132 0.71 -0.8 181,864 + GFF2132 0.71 +0.1 181,864 + GFF2132 0.71 +0.1 181,864 + GFF2132 0.71 +0.3 181,864 + GFF2132 0.71 +0.3 181,865 - GFF2132 0.71 -0.2 181,865 - GFF2132 0.71 -0.2 181,865 - GFF2132 0.71 -1.5 181,865 - GFF2132 0.71 +0.1 181,865 - GFF2132 0.71 -0.7 181,865 - GFF2132 0.71 +0.7 181,865 - GFF2132 0.71 +0.0 181,865 - GFF2132 0.71 -1.0 181,865 - GFF2132 0.71 +1.1 181,885 + GFF2132 0.74 -0.6 181,885 + GFF2132 0.74 -0.2 181,885 + GFF2132 0.74 +0.5 181,886 - GFF2132 0.74 +0.5 181,886 - GFF2132 0.74 -1.4 181,886 - GFF2132 0.74 -1.4 181,886 - GFF2132 0.74 -0.3 181,919 - GFF2132 0.78 -0.3 182,052 + -0.5 182,053 - -0.0 183,254 + -0.0 183,254 + -0.6 183,254 + -0.2 183,254 + -0.0 183,254 + +0.4 183,254 + +0.3 183,254 + -1.1 183,255 - -0.4 183,255 - +0.5 183,255 - -0.2 183,255 - -0.8 183,255 - -0.4 183,255 - +0.0 183,255 - -0.3 183,261 + +0.4 183,261 + +0.1 183,261 + -0.7 183,261 + -0.3 183,262 - +0.5 183,262 - -0.5 183,262 - -1.3 183,262 - +0.7 183,383 - -0.3 183,649 + GFF2134 0.36 +0.4 183,812 - GFF2134 0.55 +0.2 184,090 + GFF2134 0.86 +0.6 184,198 + -1.1 184,198 + -1.2 184,198 + -0.0 184,199 - -0.9 184,199 - +0.1
Or see this region's nucleotide sequence