Strain Fitness in Variovorax sp. SCN45 around GFF2115

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2114 and GFF2115 are separated by 145 nucleotidesGFF2115 and GFF2116 are separated by 244 nucleotides GFF2114 - 3-methylmercaptopropionyl-CoA ligase (EC 6.2.1.44) of DmdB2 type, at 162,894 to 164,534 GFF2114 GFF2115 - transcriptional regulator, AraC family, at 164,680 to 165,492 GFF2115 GFF2116 - Cytochrome P450, at 165,737 to 167,131 GFF2116 Position (kb) 164 165 166Strain fitness (log2 ratio) -2 -1 0 1at 164.098 kb on - strand, within GFF2114at 164.182 kb on - strand, within GFF2114at 164.182 kb on - strand, within GFF2114at 164.682 kb on - strandat 164.682 kb on - strandat 165.627 kb on - strandat 166.323 kb on - strand, within GFF2116at 166.323 kb on - strand, within GFF2116

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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164,098 - GFF2114 0.73 +1.0
164,182 - GFF2114 0.78 -0.1
164,182 - GFF2114 0.78 -2.2
164,682 - -0.8
164,682 - -0.1
165,627 - +1.0
166,323 - GFF2116 0.42 +1.0
166,323 - GFF2116 0.42 -0.5

Or see this region's nucleotide sequence