Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF206 and GFF207 are separated by 11 nucleotides GFF207 and GFF208 overlap by 4 nucleotides GFF208 and GFF209 are separated by 77 nucleotides GFF209 and GFF210 are separated by 128 nucleotides GFF210 and GFF211 are separated by 19 nucleotides
GFF206 - GMP synthase [glutamine-hydrolyzing], amidotransferase subunit (EC 6.3.5.2) / GMP synthase [glutamine-hydrolyzing], ATP pyrophosphatase subunit (EC 6.3.5.2), at 233,157 to 234,773
GFF206
GFF207 - Death on curing protein, Doc toxin, at 234,785 to 235,219
GFF207
GFF208 - Prevent host death protein, Phd antitoxin, at 235,216 to 235,464
GFF208
GFF209 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain, at 235,542 to 237,011
GFF209
GFF210 - Putative transmembrane protein, at 237,140 to 237,673
GFF210
GFF211 - protein of unknown function UPF0125, at 237,693 to 238,028
GFF211
Position (kb) 235
236
237
238 Strain fitness (log2 ratio) -2
-1
0
1
2 at 234.894 kb on + strand, within GFF207 at 234.895 kb on - strand, within GFF207 at 234.895 kb on - strand, within GFF207 at 234.906 kb on + strand, within GFF207 at 234.907 kb on - strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.138 kb on - strand, within GFF207 at 235.138 kb on - strand, within GFF207 at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.498 kb on + strand at 235.543 kb on + strand at 235.543 kb on + strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.556 kb on - strand at 235.556 kb on - strand at 237.037 kb on + strand at 237.037 kb on + strand at 237.037 kb on + strand at 237.037 kb on + strand at 237.038 kb on - strand at 237.038 kb on - strand at 237.117 kb on + strand at 237.128 kb on + strand at 237.128 kb on + strand at 237.162 kb on + strand at 237.162 kb on + strand at 237.162 kb on + strand at 237.163 kb on - strand at 237.163 kb on - strand at 237.294 kb on + strand, within GFF210 at 237.294 kb on + strand, within GFF210 at 237.295 kb on - strand, within GFF210 at 237.671 kb on + strand at 237.671 kb on + strand at 237.671 kb on + strand at 237.671 kb on + strand at 237.671 kb on + strand at 237.672 kb on - strand at 237.672 kb on - strand at 237.672 kb on - strand at 237.672 kb on - strand at 237.672 kb on - strand at 237.672 kb on - strand at 237.698 kb on - strand at 237.698 kb on - strand at 237.746 kb on + strand, within GFF211 at 237.746 kb on + strand, within GFF211 at 237.747 kb on - strand, within GFF211 at 237.747 kb on - strand, within GFF211 at 237.747 kb on - strand, within GFF211
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 234,894 + GFF207 0.25 -0.2 234,895 - GFF207 0.25 -0.4 234,895 - GFF207 0.25 -0.7 234,906 + GFF207 0.28 -1.6 234,907 - GFF207 0.28 -2.2 235,137 + GFF207 0.81 +1.2 235,137 + GFF207 0.81 -0.6 235,137 + GFF207 0.81 -0.3 235,137 + GFF207 0.81 -1.7 235,138 - GFF207 0.81 +1.2 235,138 - GFF207 0.81 +0.6 235,456 - -0.6 235,456 - -1.4 235,456 - +1.2 235,456 - -0.4 235,456 - +1.8 235,456 - -0.3 235,456 - +0.3 235,456 - -0.1 235,498 + -0.8 235,543 + +0.0 235,543 + +0.2 235,544 - -0.6 235,544 - +0.3 235,544 - +0.5 235,544 - +1.2 235,555 + -0.2 235,555 + -1.8 235,555 + -0.3 235,555 + +0.5 235,556 - +0.1 235,556 - -1.6 237,037 + -2.6 237,037 + +0.2 237,037 + -1.6 237,037 + -0.5 237,038 - -0.3 237,038 - -0.0 237,117 + +0.4 237,128 + -1.7 237,128 + +0.2 237,162 + +0.4 237,162 + +0.2 237,162 + -0.3 237,163 - -1.2 237,163 - -0.3 237,294 + GFF210 0.29 -1.3 237,294 + GFF210 0.29 -1.0 237,295 - GFF210 0.29 -0.5 237,671 + +0.3 237,671 + +0.1 237,671 + -0.2 237,671 + +0.3 237,671 + -0.1 237,672 - -0.1 237,672 - -0.5 237,672 - -0.5 237,672 - -0.6 237,672 - -0.1 237,672 - -0.2 237,698 - -0.2 237,698 - -0.2 237,746 + GFF211 0.16 -0.8 237,746 + GFF211 0.16 -0.2 237,747 - GFF211 0.16 -0.5 237,747 - GFF211 0.16 -0.4 237,747 - GFF211 0.16 -0.4
Or see this region's nucleotide sequence