Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2065 and GFF2066 are separated by 184 nucleotides GFF2066 and GFF2067 are separated by 7 nucleotides GFF2067 and GFF2068 overlap by 4 nucleotides
GFF2065 - Peptidase, M23/M37 family, at 112,903 to 114,249
GFF2065
GFF2066 - Tyrosyl-tRNA synthetase (EC 6.1.1.1), at 114,434 to 115,642
GFF2066
GFF2067 - OmpA/MotB, at 115,650 to 116,273
GFF2067
GFF2068 - hypothetical protein, at 116,270 to 116,734
GFF2068
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 113.561 kb on + strand, within GFF2065 at 113.561 kb on + strand, within GFF2065 at 113.561 kb on + strand, within GFF2065 at 113.561 kb on + strand, within GFF2065 at 113.561 kb on + strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.562 kb on - strand, within GFF2065 at 113.688 kb on + strand, within GFF2065 at 113.688 kb on + strand, within GFF2065 at 113.688 kb on + strand, within GFF2065 at 113.688 kb on + strand, within GFF2065 at 113.688 kb on + strand, within GFF2065 at 113.688 kb on + strand, within GFF2065 at 113.688 kb on + strand, within GFF2065 at 113.689 kb on - strand, within GFF2065 at 113.689 kb on - strand, within GFF2065 at 113.689 kb on - strand, within GFF2065 at 113.689 kb on - strand, within GFF2065 at 113.689 kb on - strand, within GFF2065 at 113.689 kb on - strand, within GFF2065 at 113.689 kb on - strand, within GFF2065 at 113.689 kb on - strand, within GFF2065 at 113.780 kb on + strand, within GFF2065 at 113.780 kb on + strand, within GFF2065 at 113.780 kb on + strand, within GFF2065 at 113.780 kb on + strand, within GFF2065 at 113.780 kb on + strand, within GFF2065 at 113.780 kb on + strand, within GFF2065 at 113.780 kb on + strand, within GFF2065 at 113.780 kb on + strand, within GFF2065 at 113.781 kb on - strand, within GFF2065 at 113.781 kb on - strand, within GFF2065 at 113.781 kb on - strand, within GFF2065 at 113.781 kb on - strand, within GFF2065 at 113.781 kb on - strand, within GFF2065 at 113.781 kb on - strand, within GFF2065 at 114.182 kb on + strand at 114.182 kb on + strand at 114.182 kb on + strand at 114.183 kb on - strand at 114.183 kb on - strand at 114.183 kb on - strand at 114.183 kb on - strand at 114.183 kb on - strand at 114.183 kb on - strand at 114.183 kb on - strand at 114.183 kb on - strand at 114.183 kb on - strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.195 kb on + strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.196 kb on - strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.197 kb on + strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.198 kb on - strand at 114.297 kb on + strand at 114.297 kb on + strand at 114.297 kb on + strand at 114.298 kb on - strand at 114.341 kb on + strand at 114.341 kb on + strand at 114.343 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.372 kb on + strand at 114.373 kb on - strand at 114.373 kb on - strand at 114.373 kb on - strand at 114.373 kb on - strand at 114.373 kb on - strand at 114.373 kb on - strand at 114.373 kb on - strand at 114.373 kb on - strand at 114.373 kb on - strand at 114.374 kb on + strand at 114.374 kb on + strand at 114.374 kb on + strand at 114.374 kb on + strand at 114.375 kb on - strand at 114.375 kb on - strand at 114.375 kb on - strand at 114.375 kb on - strand at 114.375 kb on - strand at 114.375 kb on - strand at 114.411 kb on + strand at 114.427 kb on + strand at 114.427 kb on + strand at 114.427 kb on + strand at 114.428 kb on - strand at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 113,561 + GFF2065 0.49 -1.1 113,561 + GFF2065 0.49 +0.8 113,561 + GFF2065 0.49 -0.8 113,561 + GFF2065 0.49 -0.1 113,561 + GFF2065 0.49 +0.2 113,562 - GFF2065 0.49 +0.6 113,562 - GFF2065 0.49 -1.5 113,562 - GFF2065 0.49 -0.3 113,562 - GFF2065 0.49 +0.1 113,562 - GFF2065 0.49 +1.0 113,562 - GFF2065 0.49 -0.9 113,562 - GFF2065 0.49 -0.3 113,562 - GFF2065 0.49 +0.5 113,562 - GFF2065 0.49 +0.5 113,562 - GFF2065 0.49 -1.4 113,562 - GFF2065 0.49 -0.4 113,562 - GFF2065 0.49 -0.4 113,562 - GFF2065 0.49 -0.4 113,562 - GFF2065 0.49 -0.2 113,688 + GFF2065 0.58 -0.1 113,688 + GFF2065 0.58 -0.6 113,688 + GFF2065 0.58 -0.6 113,688 + GFF2065 0.58 -0.6 113,688 + GFF2065 0.58 +0.5 113,688 + GFF2065 0.58 -1.6 113,688 + GFF2065 0.58 -2.6 113,689 - GFF2065 0.58 -1.6 113,689 - GFF2065 0.58 +0.1 113,689 - GFF2065 0.58 -1.1 113,689 - GFF2065 0.58 +0.1 113,689 - GFF2065 0.58 +0.5 113,689 - GFF2065 0.58 +0.3 113,689 - GFF2065 0.58 -0.8 113,689 - GFF2065 0.58 -0.5 113,780 + GFF2065 0.65 -0.2 113,780 + GFF2065 0.65 -1.1 113,780 + GFF2065 0.65 +0.3 113,780 + GFF2065 0.65 -0.6 113,780 + GFF2065 0.65 -0.3 113,780 + GFF2065 0.65 -0.5 113,780 + GFF2065 0.65 -1.3 113,780 + GFF2065 0.65 -0.2 113,781 - GFF2065 0.65 +0.1 113,781 - GFF2065 0.65 -0.1 113,781 - GFF2065 0.65 -0.4 113,781 - GFF2065 0.65 -0.5 113,781 - GFF2065 0.65 -0.3 113,781 - GFF2065 0.65 -0.7 114,182 + -0.2 114,182 + +0.4 114,182 + +0.2 114,183 - +0.4 114,183 - +0.9 114,183 - +1.1 114,183 - -0.0 114,183 - +0.5 114,183 - -0.9 114,183 - -0.0 114,183 - -0.3 114,183 - +0.9 114,195 + +0.3 114,195 + -0.7 114,195 + -0.4 114,195 + +0.4 114,195 + +0.7 114,195 + -1.3 114,195 + -0.3 114,195 + +1.1 114,195 + -0.8 114,195 + -0.4 114,195 + -0.3 114,195 + -1.2 114,195 + -0.2 114,195 + +1.7 114,195 + +0.1 114,195 + +0.7 114,195 + -0.1 114,195 + +0.1 114,195 + -0.2 114,196 - +1.1 114,196 - +0.1 114,196 - +0.1 114,196 - +0.7 114,196 - +0.2 114,196 - +0.3 114,196 - -0.9 114,196 - -0.5 114,196 - +0.6 114,196 - +0.1 114,196 - -0.2 114,196 - -0.5 114,196 - -1.5 114,196 - -0.6 114,196 - -0.9 114,196 - +0.3 114,196 - -0.5 114,196 - +0.0 114,196 - -0.6 114,196 - -1.1 114,196 - -0.2 114,196 - -1.0 114,196 - -0.2 114,196 - +3.1 114,196 - +1.3 114,196 - +0.3 114,196 - +0.3 114,196 - -0.4 114,196 - -0.2 114,196 - -0.5 114,196 - -0.3 114,196 - -0.0 114,196 - -1.6 114,196 - -1.0 114,196 - +0.6 114,196 - +2.1 114,196 - -0.5 114,196 - +0.2 114,196 - -0.3 114,196 - -0.0 114,196 - -1.1 114,196 - +0.3 114,196 - +0.2 114,196 - -0.5 114,197 + -1.9 114,197 + -0.0 114,197 + -0.5 114,197 + -0.3 114,197 + +0.3 114,197 + +0.6 114,197 + -1.5 114,197 + -0.5 114,197 + +0.5 114,197 + -0.2 114,198 - -0.1 114,198 - +0.2 114,198 - -0.5 114,198 - -0.8 114,198 - -0.5 114,198 - +0.4 114,198 - -0.3 114,198 - -0.5 114,198 - -0.8 114,198 - -0.0 114,198 - +0.5 114,198 - -1.3 114,198 - -0.1 114,198 - -0.3 114,297 + -0.5 114,297 + +0.6 114,297 + +0.5 114,298 - -0.2 114,341 + -0.3 114,341 + -0.5 114,343 + -0.1 114,372 + -0.1 114,372 + +0.2 114,372 + -0.3 114,372 + +1.7 114,372 + -0.1 114,372 + -0.1 114,372 + +0.4 114,372 + +0.4 114,372 + +0.1 114,372 + -0.2 114,372 + +0.4 114,372 + -0.3 114,372 + -0.9 114,372 + -0.1 114,373 - +0.8 114,373 - +2.4 114,373 - -0.6 114,373 - +0.3 114,373 - +0.7 114,373 - -0.3 114,373 - -2.3 114,373 - -1.6 114,373 - -0.3 114,374 + +0.3 114,374 + +0.2 114,374 + +1.3 114,374 + +0.0 114,375 - -0.8 114,375 - -1.1 114,375 - -0.7 114,375 - -0.5 114,375 - -0.2 114,375 - +0.4 114,411 + +0.1 114,427 + -0.7 114,427 + +0.2 114,427 + +0.1 114,428 - +2.3 115,960 + GFF2067 0.50 +0.0 115,960 + GFF2067 0.50 -1.6 115,960 + GFF2067 0.50 +1.2 115,960 + GFF2067 0.50 -0.2 115,960 + GFF2067 0.50 -0.4 115,961 - GFF2067 0.50 -0.2 115,961 - GFF2067 0.50 +0.6 115,961 - GFF2067 0.50 -2.1 115,961 - GFF2067 0.50 -0.4 115,961 - GFF2067 0.50 -0.3 115,961 - GFF2067 0.50 -0.1 115,961 - GFF2067 0.50 +0.0 115,961 - GFF2067 0.50 -0.2 115,961 - GFF2067 0.50 -0.3 115,961 - GFF2067 0.50 -0.1 115,961 - GFF2067 0.50 -0.6 115,961 - GFF2067 0.50 -0.7 115,961 - GFF2067 0.50 -2.9 115,969 + GFF2067 0.51 +0.1 115,969 + GFF2067 0.51 -0.2 115,969 + GFF2067 0.51 -0.1 115,969 + GFF2067 0.51 +0.1 115,970 - GFF2067 0.51 +0.9 115,970 - GFF2067 0.51 +1.3 115,970 - GFF2067 0.51 +1.3 115,970 - GFF2067 0.51 -0.1 116,093 - GFF2067 0.71 +0.1 116,093 - GFF2067 0.71 +1.1 116,093 - GFF2067 0.71 -0.5 116,093 - GFF2067 0.71 -0.2
Or see this region's nucleotide sequence