Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF204 and GFF205 are separated by 42 nucleotides GFF205 and GFF206 are separated by 277 nucleotides GFF206 and GFF207 are separated by 11 nucleotides GFF207 and GFF208 overlap by 4 nucleotides GFF208 and GFF209 are separated by 77 nucleotides
GFF204 - hypothetical protein, at 231,587 to 232,216
GFF204
GFF205 - Nitroreductase family protein, at 232,259 to 232,879
GFF205
GFF206 - GMP synthase [glutamine-hydrolyzing], amidotransferase subunit (EC 6.3.5.2) / GMP synthase [glutamine-hydrolyzing], ATP pyrophosphatase subunit (EC 6.3.5.2), at 233,157 to 234,773
GFF206
GFF207 - Death on curing protein, Doc toxin, at 234,785 to 235,219
GFF207
GFF208 - Prevent host death protein, Phd antitoxin, at 235,216 to 235,464
GFF208
GFF209 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain, at 235,542 to 237,011
GFF209
Position (kb) 233
234
235 Strain fitness (log2 ratio) -2
-1
0
1
2 at 232.214 kb on + strand at 232.214 kb on + strand at 232.214 kb on + strand at 232.215 kb on - strand at 232.215 kb on - strand at 232.215 kb on - strand at 232.215 kb on - strand at 232.215 kb on - strand at 232.371 kb on + strand, within GFF205 at 232.371 kb on + strand, within GFF205 at 232.371 kb on + strand, within GFF205 at 232.371 kb on + strand, within GFF205 at 232.372 kb on - strand, within GFF205 at 232.372 kb on - strand, within GFF205 at 232.372 kb on - strand, within GFF205 at 232.434 kb on + strand, within GFF205 at 232.434 kb on + strand, within GFF205 at 232.434 kb on + strand, within GFF205 at 232.435 kb on - strand, within GFF205 at 232.467 kb on + strand, within GFF205 at 232.468 kb on - strand, within GFF205 at 232.468 kb on - strand, within GFF205 at 233.068 kb on + strand at 233.069 kb on - strand at 233.069 kb on - strand at 233.104 kb on + strand at 233.148 kb on + strand at 233.148 kb on + strand at 233.149 kb on - strand at 233.149 kb on - strand at 234.894 kb on + strand, within GFF207 at 234.895 kb on - strand, within GFF207 at 234.895 kb on - strand, within GFF207 at 234.906 kb on + strand, within GFF207 at 234.907 kb on - strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.138 kb on - strand, within GFF207 at 235.138 kb on - strand, within GFF207 at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.498 kb on + strand at 235.543 kb on + strand at 235.543 kb on + strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.556 kb on - strand at 235.556 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 232,214 + -0.3 232,214 + +0.0 232,214 + -0.5 232,215 - +0.1 232,215 - +0.4 232,215 - -1.5 232,215 - -0.4 232,215 - -1.0 232,371 + GFF205 0.18 -1.6 232,371 + GFF205 0.18 -0.7 232,371 + GFF205 0.18 +0.0 232,371 + GFF205 0.18 +1.5 232,372 - GFF205 0.18 -0.9 232,372 - GFF205 0.18 -1.7 232,372 - GFF205 0.18 -0.5 232,434 + GFF205 0.28 +1.2 232,434 + GFF205 0.28 -0.7 232,434 + GFF205 0.28 -0.5 232,435 - GFF205 0.28 -0.8 232,467 + GFF205 0.33 -1.4 232,468 - GFF205 0.34 +1.8 232,468 - GFF205 0.34 -0.2 233,068 + -0.9 233,069 - -0.5 233,069 - -1.4 233,104 + +0.5 233,148 + -0.8 233,148 + -0.5 233,149 - +1.4 233,149 - -0.1 234,894 + GFF207 0.25 -0.2 234,895 - GFF207 0.25 -0.4 234,895 - GFF207 0.25 -0.7 234,906 + GFF207 0.28 -1.6 234,907 - GFF207 0.28 -2.2 235,137 + GFF207 0.81 +1.2 235,137 + GFF207 0.81 -0.6 235,137 + GFF207 0.81 -0.3 235,137 + GFF207 0.81 -1.7 235,138 - GFF207 0.81 +1.2 235,138 - GFF207 0.81 +0.6 235,456 - -0.6 235,456 - -1.4 235,456 - +1.2 235,456 - -0.4 235,456 - +1.8 235,456 - -0.3 235,456 - +0.3 235,456 - -0.1 235,498 + -0.8 235,543 + +0.0 235,543 + +0.2 235,544 - -0.6 235,544 - +0.3 235,544 - +0.5 235,544 - +1.2 235,555 + -0.2 235,555 + -1.8 235,555 + -0.3 235,555 + +0.5 235,556 - +0.1 235,556 - -1.6
Or see this region's nucleotide sequence