Strain Fitness in Variovorax sp. SCN45 around GFF2048

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2047 and GFF2048 overlap by 4 nucleotidesGFF2048 and GFF2049 are separated by 32 nucleotidesGFF2049 and GFF2050 are separated by 26 nucleotides GFF2047 - Polyhydroxyalkanoic acid synthase, at 95,473 to 96,546 GFF2047 GFF2048 - hypothetical protein, at 96,543 to 96,950 GFF2048 GFF2049 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 96,983 to 97,903 GFF2049 GFF2050 - hypothetical protein, at 97,930 to 98,430 GFF2050 Position (kb) 96 97Strain fitness (log2 ratio) -2 -1 0 1at 95.627 kb on + strand, within GFF2047at 95.628 kb on - strand, within GFF2047at 95.628 kb on - strand, within GFF2047at 95.771 kb on + strand, within GFF2047at 95.771 kb on + strand, within GFF2047at 95.771 kb on + strand, within GFF2047at 95.772 kb on - strand, within GFF2047at 95.772 kb on - strand, within GFF2047at 95.772 kb on - strand, within GFF2047at 95.972 kb on + strand, within GFF2047at 96.078 kb on - strand, within GFF2047at 96.185 kb on + strand, within GFF2047at 96.186 kb on - strand, within GFF2047at 96.186 kb on - strand, within GFF2047at 96.186 kb on - strand, within GFF2047at 96.242 kb on + strand, within GFF2047at 96.242 kb on + strand, within GFF2047at 96.242 kb on + strand, within GFF2047at 96.242 kb on + strand, within GFF2047at 96.242 kb on + strand, within GFF2047at 96.242 kb on + strand, within GFF2047at 96.243 kb on - strand, within GFF2047at 96.251 kb on + strand, within GFF2047at 96.251 kb on + strand, within GFF2047at 96.252 kb on - strand, within GFF2047at 96.269 kb on + strand, within GFF2047at 96.269 kb on + strand, within GFF2047at 96.269 kb on + strand, within GFF2047at 96.270 kb on - strand, within GFF2047at 96.308 kb on + strand, within GFF2047at 96.308 kb on + strand, within GFF2047at 96.308 kb on + strand, within GFF2047at 96.309 kb on - strand, within GFF2047at 96.309 kb on - strand, within GFF2047at 96.354 kb on - strand, within GFF2047at 96.354 kb on - strand, within GFF2047at 96.377 kb on + strand, within GFF2047at 96.378 kb on - strand, within GFF2047at 96.383 kb on + strand, within GFF2047at 96.383 kb on + strand, within GFF2047at 96.384 kb on - strand, within GFF2047at 96.384 kb on - strand, within GFF2047at 96.384 kb on - strand, within GFF2047at 96.384 kb on - strand, within GFF2047at 97.362 kb on + strand, within GFF2049at 97.363 kb on - strand, within GFF2049at 97.363 kb on - strand, within GFF2049at 97.363 kb on - strand, within GFF2049at 97.363 kb on - strand, within GFF2049at 97.605 kb on + strand, within GFF2049at 97.606 kb on - strand, within GFF2049at 97.606 kb on - strand, within GFF2049at 97.606 kb on - strand, within GFF2049at 97.606 kb on - strand, within GFF2049at 97.931 kb on + strandat 97.931 kb on + strandat 97.931 kb on + strandat 97.931 kb on + strandat 97.931 kb on + strandat 97.931 kb on + strandat 97.932 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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95,627 + GFF2047 0.14 +0.2
95,628 - GFF2047 0.14 -0.6
95,628 - GFF2047 0.14 -2.7
95,771 + GFF2047 0.28 +0.3
95,771 + GFF2047 0.28 +0.3
95,771 + GFF2047 0.28 -0.3
95,772 - GFF2047 0.28 -0.6
95,772 - GFF2047 0.28 -0.2
95,772 - GFF2047 0.28 -0.4
95,972 + GFF2047 0.46 -0.7
96,078 - GFF2047 0.56 -0.1
96,185 + GFF2047 0.66 -0.3
96,186 - GFF2047 0.66 +0.0
96,186 - GFF2047 0.66 -0.3
96,186 - GFF2047 0.66 +0.0
96,242 + GFF2047 0.72 -1.1
96,242 + GFF2047 0.72 -0.1
96,242 + GFF2047 0.72 -0.1
96,242 + GFF2047 0.72 -0.4
96,242 + GFF2047 0.72 -0.3
96,242 + GFF2047 0.72 +0.1
96,243 - GFF2047 0.72 +0.0
96,251 + GFF2047 0.72 +0.1
96,251 + GFF2047 0.72 +0.6
96,252 - GFF2047 0.73 -0.0
96,269 + GFF2047 0.74 -0.8
96,269 + GFF2047 0.74 -0.2
96,269 + GFF2047 0.74 +0.9
96,270 - GFF2047 0.74 +0.7
96,308 + GFF2047 0.78 +0.9
96,308 + GFF2047 0.78 +1.3
96,308 + GFF2047 0.78 +0.6
96,309 - GFF2047 0.78 -0.0
96,309 - GFF2047 0.78 -0.2
96,354 - GFF2047 0.82 +0.7
96,354 - GFF2047 0.82 +0.1
96,377 + GFF2047 0.84 -0.8
96,378 - GFF2047 0.84 -0.3
96,383 + GFF2047 0.85 -1.6
96,383 + GFF2047 0.85 -0.7
96,384 - GFF2047 0.85 -1.1
96,384 - GFF2047 0.85 +0.7
96,384 - GFF2047 0.85 +0.0
96,384 - GFF2047 0.85 +0.2
97,362 + GFF2049 0.41 -0.6
97,363 - GFF2049 0.41 +0.5
97,363 - GFF2049 0.41 -0.3
97,363 - GFF2049 0.41 +0.6
97,363 - GFF2049 0.41 +0.4
97,605 + GFF2049 0.68 -1.5
97,606 - GFF2049 0.68 +0.4
97,606 - GFF2049 0.68 +1.4
97,606 - GFF2049 0.68 +0.0
97,606 - GFF2049 0.68 -0.3
97,931 + +1.7
97,931 + -0.3
97,931 + +0.1
97,931 + +1.4
97,931 + -0.3
97,931 + -0.3
97,932 - +0.5

Or see this region's nucleotide sequence