Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1980 and GFF1981 are separated by 25 nucleotides GFF1981 and GFF1982 are separated by 13 nucleotides GFF1982 and GFF1983 overlap by 4 nucleotides
GFF1980 - Glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 22,389 to 24,533
GFF1980
GFF1981 - no description, at 24,559 to 24,888
GFF1981
GFF1982 - no description, at 24,902 to 25,411
GFF1982
GFF1983 - D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (EC 3.1.3.82); Histidinol-phosphatase (EC 3.1.3.15), at 25,408 to 25,986
GFF1983
Position (kb)
24
25 Strain fitness (log2 ratio)
-1
0
1 at 24.886 kb on + strand at 24.887 kb on - strand at 25.178 kb on + strand, within GFF1982 at 25.178 kb on + strand, within GFF1982 at 25.178 kb on + strand, within GFF1982 at 25.178 kb on + strand, within GFF1982 at 25.178 kb on + strand, within GFF1982 at 25.178 kb on + strand, within GFF1982 at 25.178 kb on + strand, within GFF1982 at 25.179 kb on - strand, within GFF1982 at 25.179 kb on - strand, within GFF1982 at 25.179 kb on - strand, within GFF1982 at 25.179 kb on - strand, within GFF1982 at 25.179 kb on - strand, within GFF1982 at 25.179 kb on - strand, within GFF1982 at 25.179 kb on - strand, within GFF1982 at 25.179 kb on - strand, within GFF1982 at 25.347 kb on - strand, within GFF1982 at 25.669 kb on + strand, within GFF1983 at 25.669 kb on + strand, within GFF1983 at 25.669 kb on + strand, within GFF1983 at 25.669 kb on + strand, within GFF1983 at 25.669 kb on + strand, within GFF1983 at 25.669 kb on + strand, within GFF1983 at 25.669 kb on + strand, within GFF1983 at 25.670 kb on - strand, within GFF1983 at 25.670 kb on - strand, within GFF1983 at 25.670 kb on - strand, within GFF1983 at 25.670 kb on - strand, within GFF1983 at 25.670 kb on - strand, within GFF1983 at 25.670 kb on - strand, within GFF1983 at 25.670 kb on - strand, within GFF1983
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 24,886 + +0.3 24,887 - -0.6 25,178 + GFF1982 0.54 -0.4 25,178 + GFF1982 0.54 +0.4 25,178 + GFF1982 0.54 -1.3 25,178 + GFF1982 0.54 -1.2 25,178 + GFF1982 0.54 -1.6 25,178 + GFF1982 0.54 +0.7 25,178 + GFF1982 0.54 +0.3 25,179 - GFF1982 0.54 -0.3 25,179 - GFF1982 0.54 -0.6 25,179 - GFF1982 0.54 +0.8 25,179 - GFF1982 0.54 +0.4 25,179 - GFF1982 0.54 -0.3 25,179 - GFF1982 0.54 +0.1 25,179 - GFF1982 0.54 -1.3 25,179 - GFF1982 0.54 -1.4 25,347 - GFF1982 0.87 -0.3 25,669 + GFF1983 0.45 -0.2 25,669 + GFF1983 0.45 -0.7 25,669 + GFF1983 0.45 -0.9 25,669 + GFF1983 0.45 +0.0 25,669 + GFF1983 0.45 +0.2 25,669 + GFF1983 0.45 -0.5 25,669 + GFF1983 0.45 -0.7 25,670 - GFF1983 0.45 -0.8 25,670 - GFF1983 0.45 -0.2 25,670 - GFF1983 0.45 +0.4 25,670 - GFF1983 0.45 +0.1 25,670 - GFF1983 0.45 +1.7 25,670 - GFF1983 0.45 +0.2 25,670 - GFF1983 0.45 -1.1
Or see this region's nucleotide sequence