Strain Fitness in Variovorax sp. SCN45 around GFF1979

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1978 and GFF1979 are separated by 182 nucleotidesGFF1979 and GFF1980 are separated by 3 nucleotides GFF1978 - putative membrane-associated phospholipid phosphatase, PAP2 superfamily, at 20,482 to 21,294 GFF1978 GFF1979 - Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14), at 21,477 to 22,385 GFF1979 GFF1980 - Glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 22,389 to 24,533 GFF1980 Position (kb) 21 22 23Strain fitness (log2 ratio) -1 0 1at 20.510 kb on + strandat 20.583 kb on - strand, within GFF1978at 20.690 kb on + strand, within GFF1978at 20.690 kb on + strand, within GFF1978at 20.691 kb on - strand, within GFF1978at 20.691 kb on - strand, within GFF1978at 20.705 kb on + strand, within GFF1978at 20.705 kb on + strand, within GFF1978at 20.706 kb on - strand, within GFF1978at 20.706 kb on - strand, within GFF1978at 20.706 kb on - strand, within GFF1978at 20.747 kb on + strand, within GFF1978at 20.748 kb on - strand, within GFF1978at 21.217 kb on + strandat 21.217 kb on + strandat 21.217 kb on + strandat 21.378 kb on + strandat 21.410 kb on + strandat 21.411 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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20,510 + -0.9
20,583 - GFF1978 0.12 +0.3
20,690 + GFF1978 0.26 +0.6
20,690 + GFF1978 0.26 -0.5
20,691 - GFF1978 0.26 +0.0
20,691 - GFF1978 0.26 -0.9
20,705 + GFF1978 0.27 +0.1
20,705 + GFF1978 0.27 +1.1
20,706 - GFF1978 0.28 +1.4
20,706 - GFF1978 0.28 -0.6
20,706 - GFF1978 0.28 -0.7
20,747 + GFF1978 0.33 -0.8
20,748 - GFF1978 0.33 -1.1
21,217 + -0.0
21,217 + -0.4
21,217 + +0.0
21,378 + -1.2
21,410 + +0.4
21,411 - -0.7

Or see this region's nucleotide sequence