Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1972 and GFF1973 overlap by 4 nucleotides GFF1973 and GFF1974 are separated by 169 nucleotides GFF1974 and GFF1975 are separated by 23 nucleotides
GFF1972 - Cytosine deaminase (EC 3.5.4.1), at 14,833 to 16,110
GFF1972
GFF1973 - NAD(P)H oxidoreductase YrkL @ Putative NADPH-quinone reductase (modulator of drug activity B) @ Flavodoxin 2, at 16,107 to 16,694
GFF1973
GFF1974 - hypothetical protein, at 16,864 to 17,187
GFF1974
GFF1975 - hypothetical protein, at 17,211 to 18,329
GFF1975
Position (kb)
16
17 Strain fitness (log2 ratio)
-2
-1
0
1 at 16.186 kb on + strand, within GFF1973 at 16.186 kb on + strand, within GFF1973 at 16.187 kb on - strand, within GFF1973 at 16.198 kb on + strand, within GFF1973 at 16.199 kb on - strand, within GFF1973 at 16.199 kb on - strand, within GFF1973 at 16.237 kb on + strand, within GFF1973 at 16.237 kb on + strand, within GFF1973 at 16.237 kb on + strand, within GFF1973 at 16.238 kb on - strand, within GFF1973 at 16.238 kb on - strand, within GFF1973 at 16.238 kb on - strand, within GFF1973 at 16.238 kb on - strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.300 kb on + strand, within GFF1973 at 16.300 kb on + strand, within GFF1973 at 16.301 kb on - strand, within GFF1973 at 16.406 kb on - strand, within GFF1973 at 16.489 kb on + strand, within GFF1973 at 16.489 kb on + strand, within GFF1973 at 16.528 kb on + strand, within GFF1973 at 16.576 kb on + strand, within GFF1973 at 16.576 kb on + strand, within GFF1973 at 16.676 kb on - strand at 16.711 kb on + strand at 16.711 kb on + strand at 16.712 kb on - strand at 16.734 kb on - strand at 16.734 kb on - strand at 16.864 kb on - strand at 16.864 kb on - strand at 16.864 kb on - strand at 16.864 kb on - strand at 17.053 kb on + strand, within GFF1974 at 17.053 kb on + strand, within GFF1974 at 17.053 kb on + strand, within GFF1974 at 17.053 kb on + strand, within GFF1974 at 17.059 kb on + strand, within GFF1974 at 17.108 kb on - strand, within GFF1974 at 17.185 kb on + strand at 17.185 kb on + strand at 17.185 kb on + strand at 17.186 kb on - strand at 17.186 kb on - strand at 17.186 kb on - strand at 17.311 kb on + strand at 17.311 kb on + strand at 17.312 kb on - strand at 17.312 kb on - strand at 17.401 kb on + strand, within GFF1975 at 17.401 kb on + strand, within GFF1975 at 17.437 kb on + strand, within GFF1975 at 17.438 kb on - strand, within GFF1975 at 17.438 kb on - strand, within GFF1975 at 17.438 kb on - strand, within GFF1975 at 17.443 kb on + strand, within GFF1975 at 17.582 kb on - strand, within GFF1975 at 17.620 kb on + strand, within GFF1975 at 17.620 kb on + strand, within GFF1975 at 17.620 kb on + strand, within GFF1975 at 17.620 kb on + strand, within GFF1975 at 17.620 kb on + strand, within GFF1975 at 17.621 kb on - strand, within GFF1975 at 17.621 kb on - strand, within GFF1975 at 17.621 kb on - strand, within GFF1975 at 17.621 kb on - strand, within GFF1975
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 16,186 + GFF1973 0.13 +0.1 16,186 + GFF1973 0.13 +0.9 16,187 - GFF1973 0.14 -2.0 16,198 + GFF1973 0.15 -0.0 16,199 - GFF1973 0.16 +0.1 16,199 - GFF1973 0.16 -1.9 16,237 + GFF1973 0.22 +0.9 16,237 + GFF1973 0.22 -0.1 16,237 + GFF1973 0.22 +0.0 16,238 - GFF1973 0.22 -0.8 16,238 - GFF1973 0.22 +1.5 16,238 - GFF1973 0.22 +0.4 16,238 - GFF1973 0.22 -0.0 16,273 + GFF1973 0.28 +1.4 16,273 + GFF1973 0.28 -0.5 16,273 + GFF1973 0.28 +0.1 16,273 + GFF1973 0.28 -0.7 16,273 + GFF1973 0.28 +1.3 16,273 + GFF1973 0.28 -0.3 16,273 + GFF1973 0.28 +0.8 16,273 + GFF1973 0.28 +0.4 16,273 + GFF1973 0.28 +0.2 16,273 + GFF1973 0.28 -0.0 16,273 + GFF1973 0.28 +0.6 16,273 + GFF1973 0.28 -0.4 16,273 + GFF1973 0.28 +0.0 16,274 - GFF1973 0.28 +0.4 16,274 - GFF1973 0.28 +1.4 16,274 - GFF1973 0.28 +0.1 16,274 - GFF1973 0.28 -0.3 16,274 - GFF1973 0.28 +0.3 16,274 - GFF1973 0.28 +0.4 16,274 - GFF1973 0.28 -0.8 16,274 - GFF1973 0.28 -0.2 16,274 - GFF1973 0.28 -0.5 16,274 - GFF1973 0.28 +0.5 16,274 - GFF1973 0.28 +0.3 16,274 - GFF1973 0.28 +0.2 16,274 - GFF1973 0.28 +0.5 16,274 - GFF1973 0.28 -1.2 16,274 - GFF1973 0.28 +0.3 16,274 - GFF1973 0.28 +0.3 16,274 - GFF1973 0.28 +0.2 16,300 + GFF1973 0.33 +0.8 16,300 + GFF1973 0.33 +0.0 16,301 - GFF1973 0.33 +1.0 16,406 - GFF1973 0.51 -0.7 16,489 + GFF1973 0.65 +1.4 16,489 + GFF1973 0.65 +0.3 16,528 + GFF1973 0.72 -0.5 16,576 + GFF1973 0.80 +0.9 16,576 + GFF1973 0.80 +0.0 16,676 - -1.7 16,711 + +0.6 16,711 + +0.2 16,712 - +0.2 16,734 - +1.1 16,734 - -0.1 16,864 - -2.2 16,864 - +0.2 16,864 - +0.0 16,864 - -0.7 17,053 + GFF1974 0.58 +0.2 17,053 + GFF1974 0.58 -0.3 17,053 + GFF1974 0.58 -0.2 17,053 + GFF1974 0.58 -0.5 17,059 + GFF1974 0.60 -0.6 17,108 - GFF1974 0.75 +0.1 17,185 + -0.2 17,185 + -1.8 17,185 + +0.5 17,186 - -0.3 17,186 - +0.5 17,186 - -0.1 17,311 + -0.6 17,311 + -0.5 17,312 - -0.1 17,312 - +1.1 17,401 + GFF1975 0.17 -1.9 17,401 + GFF1975 0.17 -1.3 17,437 + GFF1975 0.20 +1.0 17,438 - GFF1975 0.20 -0.1 17,438 - GFF1975 0.20 -0.2 17,438 - GFF1975 0.20 +0.1 17,443 + GFF1975 0.21 -0.6 17,582 - GFF1975 0.33 +1.0 17,620 + GFF1975 0.37 -0.3 17,620 + GFF1975 0.37 -0.3 17,620 + GFF1975 0.37 +0.2 17,620 + GFF1975 0.37 +0.8 17,620 + GFF1975 0.37 +0.1 17,621 - GFF1975 0.37 -0.3 17,621 - GFF1975 0.37 +0.3 17,621 - GFF1975 0.37 +1.0 17,621 - GFF1975 0.37 -0.4
Or see this region's nucleotide sequence