Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1970 and GFF1971 are separated by 75 nucleotides GFF1971 and GFF1972 are separated by 17 nucleotides GFF1972 and GFF1973 overlap by 4 nucleotides GFF1973 and GFF1974 are separated by 169 nucleotides
GFF1970 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 12,893 to 13,876
GFF1970
GFF1971 - Creatinine amidohydrolase (EC 3.5.2.10), at 13,952 to 14,815
GFF1971
GFF1972 - Cytosine deaminase (EC 3.5.4.1), at 14,833 to 16,110
GFF1972
GFF1973 - NAD(P)H oxidoreductase YrkL @ Putative NADPH-quinone reductase (modulator of drug activity B) @ Flavodoxin 2, at 16,107 to 16,694
GFF1973
GFF1974 - hypothetical protein, at 16,864 to 17,187
GFF1974
Position (kb)
14
15
16
17 Strain fitness (log2 ratio)
-2
-1
0
1 at 14.485 kb on - strand, within GFF1971 at 14.581 kb on - strand, within GFF1971 at 14.803 kb on - strand at 14.803 kb on - strand at 14.803 kb on - strand at 14.834 kb on + strand at 14.835 kb on - strand at 16.186 kb on + strand, within GFF1973 at 16.186 kb on + strand, within GFF1973 at 16.187 kb on - strand, within GFF1973 at 16.198 kb on + strand, within GFF1973 at 16.199 kb on - strand, within GFF1973 at 16.199 kb on - strand, within GFF1973 at 16.237 kb on + strand, within GFF1973 at 16.237 kb on + strand, within GFF1973 at 16.237 kb on + strand, within GFF1973 at 16.238 kb on - strand, within GFF1973 at 16.238 kb on - strand, within GFF1973 at 16.238 kb on - strand, within GFF1973 at 16.238 kb on - strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.273 kb on + strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.274 kb on - strand, within GFF1973 at 16.300 kb on + strand, within GFF1973 at 16.300 kb on + strand, within GFF1973 at 16.301 kb on - strand, within GFF1973 at 16.406 kb on - strand, within GFF1973 at 16.489 kb on + strand, within GFF1973 at 16.489 kb on + strand, within GFF1973 at 16.528 kb on + strand, within GFF1973 at 16.576 kb on + strand, within GFF1973 at 16.576 kb on + strand, within GFF1973 at 16.676 kb on - strand at 16.711 kb on + strand at 16.711 kb on + strand at 16.712 kb on - strand at 16.734 kb on - strand at 16.734 kb on - strand at 16.864 kb on - strand at 16.864 kb on - strand at 16.864 kb on - strand at 16.864 kb on - strand at 17.053 kb on + strand, within GFF1974 at 17.053 kb on + strand, within GFF1974 at 17.053 kb on + strand, within GFF1974 at 17.053 kb on + strand, within GFF1974 at 17.059 kb on + strand, within GFF1974 at 17.108 kb on - strand, within GFF1974
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 14,485 - GFF1971 0.62 -1.2 14,581 - GFF1971 0.73 +0.3 14,803 - -0.4 14,803 - +0.4 14,803 - -0.7 14,834 + -0.0 14,835 - +1.7 16,186 + GFF1973 0.13 +0.1 16,186 + GFF1973 0.13 +0.9 16,187 - GFF1973 0.14 -2.0 16,198 + GFF1973 0.15 -0.0 16,199 - GFF1973 0.16 +0.1 16,199 - GFF1973 0.16 -1.9 16,237 + GFF1973 0.22 +0.9 16,237 + GFF1973 0.22 -0.1 16,237 + GFF1973 0.22 +0.0 16,238 - GFF1973 0.22 -0.8 16,238 - GFF1973 0.22 +1.5 16,238 - GFF1973 0.22 +0.4 16,238 - GFF1973 0.22 -0.0 16,273 + GFF1973 0.28 +1.4 16,273 + GFF1973 0.28 -0.5 16,273 + GFF1973 0.28 +0.1 16,273 + GFF1973 0.28 -0.7 16,273 + GFF1973 0.28 +1.3 16,273 + GFF1973 0.28 -0.3 16,273 + GFF1973 0.28 +0.8 16,273 + GFF1973 0.28 +0.4 16,273 + GFF1973 0.28 +0.2 16,273 + GFF1973 0.28 -0.0 16,273 + GFF1973 0.28 +0.6 16,273 + GFF1973 0.28 -0.4 16,273 + GFF1973 0.28 +0.0 16,274 - GFF1973 0.28 +0.4 16,274 - GFF1973 0.28 +1.4 16,274 - GFF1973 0.28 +0.1 16,274 - GFF1973 0.28 -0.3 16,274 - GFF1973 0.28 +0.3 16,274 - GFF1973 0.28 +0.4 16,274 - GFF1973 0.28 -0.8 16,274 - GFF1973 0.28 -0.2 16,274 - GFF1973 0.28 -0.5 16,274 - GFF1973 0.28 +0.5 16,274 - GFF1973 0.28 +0.3 16,274 - GFF1973 0.28 +0.2 16,274 - GFF1973 0.28 +0.5 16,274 - GFF1973 0.28 -1.2 16,274 - GFF1973 0.28 +0.3 16,274 - GFF1973 0.28 +0.3 16,274 - GFF1973 0.28 +0.2 16,300 + GFF1973 0.33 +0.8 16,300 + GFF1973 0.33 +0.0 16,301 - GFF1973 0.33 +1.0 16,406 - GFF1973 0.51 -0.7 16,489 + GFF1973 0.65 +1.4 16,489 + GFF1973 0.65 +0.3 16,528 + GFF1973 0.72 -0.5 16,576 + GFF1973 0.80 +0.9 16,576 + GFF1973 0.80 +0.0 16,676 - -1.7 16,711 + +0.6 16,711 + +0.2 16,712 - +0.2 16,734 - +1.1 16,734 - -0.1 16,864 - -2.2 16,864 - +0.2 16,864 - +0.0 16,864 - -0.7 17,053 + GFF1974 0.58 +0.2 17,053 + GFF1974 0.58 -0.3 17,053 + GFF1974 0.58 -0.2 17,053 + GFF1974 0.58 -0.5 17,059 + GFF1974 0.60 -0.6 17,108 - GFF1974 0.75 +0.1
Or see this region's nucleotide sequence