Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1886 and GFF1887 are separated by 16 nucleotides GFF1887 and GFF1888 are separated by 4 nucleotides
GFF1886 - Elongation factor G-like protein TM_1651, at 560,579 to 562,639
GFF1886
GFF1887 - Fumarate hydratase class II (EC 4.2.1.2), at 562,656 to 564,038
GFF1887
GFF1888 - BUG/TctC family periplasmic protein, at 564,043 to 565,032
GFF1888
Position (kb)
562
563
564
565 Strain fitness (log2 ratio)
-1
0
1
2 at 561.897 kb on - strand, within GFF1886 at 561.950 kb on + strand, within GFF1886 at 561.951 kb on - strand, within GFF1886 at 562.129 kb on - strand, within GFF1886 at 562.244 kb on + strand, within GFF1886 at 562.244 kb on + strand, within GFF1886 at 562.245 kb on - strand, within GFF1886 at 562.245 kb on - strand, within GFF1886 at 562.245 kb on - strand, within GFF1886 at 562.245 kb on - strand, within GFF1886 at 562.508 kb on + strand at 562.551 kb on - strand at 562.616 kb on + strand at 562.617 kb on - strand at 562.690 kb on + strand at 562.691 kb on - strand at 562.691 kb on - strand at 562.691 kb on - strand at 562.691 kb on - strand at 563.943 kb on + strand at 563.943 kb on + strand at 563.977 kb on + strand at 564.132 kb on - strand at 564.132 kb on - strand at 564.154 kb on + strand, within GFF1888 at 564.154 kb on + strand, within GFF1888 at 564.154 kb on + strand, within GFF1888 at 564.154 kb on + strand, within GFF1888 at 564.155 kb on - strand, within GFF1888 at 564.209 kb on + strand, within GFF1888 at 564.209 kb on + strand, within GFF1888 at 564.209 kb on + strand, within GFF1888 at 564.260 kb on + strand, within GFF1888 at 564.261 kb on - strand, within GFF1888 at 564.473 kb on + strand, within GFF1888 at 564.473 kb on + strand, within GFF1888 at 564.473 kb on + strand, within GFF1888 at 564.474 kb on - strand, within GFF1888 at 564.699 kb on - strand, within GFF1888 at 564.714 kb on - strand, within GFF1888
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 561,897 - GFF1886 0.64 +0.2 561,950 + GFF1886 0.67 +0.5 561,951 - GFF1886 0.67 +1.0 562,129 - GFF1886 0.75 -0.2 562,244 + GFF1886 0.81 -0.2 562,244 + GFF1886 0.81 +0.9 562,245 - GFF1886 0.81 -0.4 562,245 - GFF1886 0.81 -0.9 562,245 - GFF1886 0.81 +1.3 562,245 - GFF1886 0.81 -0.0 562,508 + +0.1 562,551 - +0.3 562,616 + -0.8 562,617 - +0.5 562,690 + -0.5 562,691 - -0.4 562,691 - +0.2 562,691 - -0.7 562,691 - +0.1 563,943 + -0.5 563,943 + -0.3 563,977 + -1.7 564,132 - +0.9 564,132 - +0.6 564,154 + GFF1888 0.11 -1.0 564,154 + GFF1888 0.11 -0.3 564,154 + GFF1888 0.11 -0.5 564,154 + GFF1888 0.11 +0.1 564,155 - GFF1888 0.11 -0.3 564,209 + GFF1888 0.17 +0.0 564,209 + GFF1888 0.17 -0.9 564,209 + GFF1888 0.17 +0.5 564,260 + GFF1888 0.22 +0.7 564,261 - GFF1888 0.22 +0.1 564,473 + GFF1888 0.43 -1.1 564,473 + GFF1888 0.43 -0.8 564,473 + GFF1888 0.43 +2.0 564,474 - GFF1888 0.44 -0.4 564,699 - GFF1888 0.66 -0.4 564,714 - GFF1888 0.68 +0.9
Or see this region's nucleotide sequence