Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF185 and GFF186 are separated by 27 nucleotides GFF186 and GFF187 overlap by 4 nucleotides GFF187 and GFF188 are separated by 37 nucleotides GFF188 and GFF189 are separated by 1 nucleotides GFF189 and GFF190 are separated by 43 nucleotides
GFF185 - hypothetical protein, at 216,820 to 217,083
GFF185
GFF186 - Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13), at 217,111 to 218,085
GFF186
GFF187 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.33) / Domain of unknown function, at 218,082 to 219,155
GFF187
GFF188 - no description, at 219,193 to 219,420
GFF188
GFF189 - Negative transcriptional regulator, at 219,422 to 220,066
GFF189
GFF190 - Ankyrin, at 220,110 to 220,808
GFF190
Position (kb) 218
219
220 Strain fitness (log2 ratio) -1
0
1
2 at 218.095 kb on + strand at 218.095 kb on + strand at 218.648 kb on - strand, within GFF187 at 219.514 kb on - strand, within GFF189 at 219.663 kb on + strand, within GFF189 at 219.663 kb on + strand, within GFF189 at 219.664 kb on - strand, within GFF189 at 219.762 kb on + strand, within GFF189 at 219.763 kb on - strand, within GFF189 at 219.763 kb on - strand, within GFF189 at 219.763 kb on - strand, within GFF189 at 219.763 kb on - strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.813 kb on + strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.814 kb on - strand, within GFF189 at 219.864 kb on + strand, within GFF189 at 219.865 kb on - strand, within GFF189 at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.062 kb on + strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.063 kb on - strand at 220.111 kb on + strand at 220.112 kb on - strand at 220.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 218,095 + -0.3 218,095 + -0.8 218,648 - GFF187 0.53 -1.1 219,514 - GFF189 0.14 -0.2 219,663 + GFF189 0.37 -1.4 219,663 + GFF189 0.37 -1.3 219,664 - GFF189 0.38 -0.1 219,762 + GFF189 0.53 +1.2 219,763 - GFF189 0.53 -0.5 219,763 - GFF189 0.53 -0.0 219,763 - GFF189 0.53 +0.3 219,763 - GFF189 0.53 -0.8 219,813 + GFF189 0.61 -0.6 219,813 + GFF189 0.61 +0.6 219,813 + GFF189 0.61 -0.2 219,813 + GFF189 0.61 -0.0 219,813 + GFF189 0.61 -0.5 219,813 + GFF189 0.61 +0.9 219,813 + GFF189 0.61 +0.1 219,813 + GFF189 0.61 +1.1 219,813 + GFF189 0.61 -1.6 219,813 + GFF189 0.61 +1.2 219,813 + GFF189 0.61 -0.9 219,813 + GFF189 0.61 -1.1 219,813 + GFF189 0.61 +0.6 219,813 + GFF189 0.61 -0.4 219,813 + GFF189 0.61 -0.2 219,813 + GFF189 0.61 -0.1 219,813 + GFF189 0.61 +0.3 219,813 + GFF189 0.61 +2.0 219,813 + GFF189 0.61 -0.2 219,813 + GFF189 0.61 +0.9 219,814 - GFF189 0.61 -1.1 219,814 - GFF189 0.61 -0.6 219,814 - GFF189 0.61 -1.0 219,814 - GFF189 0.61 +1.2 219,814 - GFF189 0.61 -1.6 219,814 - GFF189 0.61 -0.1 219,814 - GFF189 0.61 -0.3 219,814 - GFF189 0.61 -0.6 219,814 - GFF189 0.61 -1.0 219,814 - GFF189 0.61 -0.1 219,814 - GFF189 0.61 -0.3 219,814 - GFF189 0.61 +1.0 219,814 - GFF189 0.61 -1.3 219,814 - GFF189 0.61 -0.4 219,814 - GFF189 0.61 -0.0 219,814 - GFF189 0.61 +0.4 219,814 - GFF189 0.61 -0.1 219,814 - GFF189 0.61 -0.0 219,814 - GFF189 0.61 +0.2 219,814 - GFF189 0.61 -0.0 219,814 - GFF189 0.61 +0.0 219,864 + GFF189 0.69 -0.3 219,865 - GFF189 0.69 +0.2 220,062 + +1.2 220,062 + -0.3 220,062 + +1.0 220,062 + +0.5 220,062 + +0.3 220,062 + +0.5 220,062 + -0.5 220,062 + +0.2 220,062 + +0.2 220,062 + +0.6 220,062 + -0.0 220,062 + +0.2 220,062 + +0.3 220,062 + -0.2 220,062 + -0.1 220,062 + +0.6 220,062 + -0.6 220,062 + +0.1 220,062 + +1.4 220,062 + -1.2 220,062 + +0.4 220,062 + -0.4 220,062 + -0.4 220,062 + -0.1 220,062 + +0.1 220,063 - -0.1 220,063 - +0.2 220,063 - +0.5 220,063 - +2.2 220,063 - +0.0 220,063 - -0.5 220,063 - -0.9 220,063 - +0.8 220,063 - +0.1 220,063 - -1.0 220,063 - -0.0 220,111 + +0.4 220,112 - +0.2 220,112 - -0.4
Or see this region's nucleotide sequence