Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF179 and GFF180 are separated by 5 nucleotides GFF180 and GFF181 are separated by 14 nucleotides
GFF179 - D-mannonate oxidoreductase (EC 1.1.1.57), at 209,652 to 211,154
GFF179
GFF180 - no description, at 211,160 to 211,489
GFF180
GFF181 - Protein of unknown function (DUF1501), at 211,504 to 212,961
GFF181
Position (kb)
211
212 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 210.582 kb on + strand, within GFF179 at 210.583 kb on - strand, within GFF179 at 210.897 kb on + strand, within GFF179 at 210.897 kb on + strand, within GFF179 at 210.898 kb on - strand, within GFF179 at 210.898 kb on - strand, within GFF179 at 210.898 kb on - strand, within GFF179 at 210.898 kb on - strand, within GFF179 at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.186 kb on - strand at 211.186 kb on - strand at 211.302 kb on + strand, within GFF180 at 211.302 kb on + strand, within GFF180 at 211.637 kb on + strand at 211.638 kb on - strand at 211.740 kb on - strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.890 kb on - strand, within GFF181 at 211.890 kb on - strand, within GFF181 at 211.890 kb on - strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.165 kb on + strand, within GFF181 at 212.165 kb on + strand, within GFF181 at 212.166 kb on - strand, within GFF181 at 212.181 kb on + strand, within GFF181 at 212.181 kb on + strand, within GFF181 at 212.182 kb on - strand, within GFF181 at 212.240 kb on + strand, within GFF181 at 212.303 kb on + strand, within GFF181 at 212.303 kb on + strand, within GFF181 at 212.303 kb on + strand, within GFF181 at 212.304 kb on - strand, within GFF181 at 212.304 kb on - strand, within GFF181
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 210,582 + GFF179 0.62 +0.1 210,583 - GFF179 0.62 -0.6 210,897 + GFF179 0.83 -1.1 210,897 + GFF179 0.83 +0.0 210,898 - GFF179 0.83 -0.2 210,898 - GFF179 0.83 +1.0 210,898 - GFF179 0.83 -0.1 210,898 - GFF179 0.83 -2.1 211,185 + +0.3 211,185 + +0.2 211,185 + -0.1 211,185 + -1.0 211,185 + -0.5 211,185 + +1.6 211,185 + -0.2 211,185 + -0.4 211,185 + +1.1 211,185 + -0.2 211,185 + -0.5 211,186 - +0.1 211,186 - -0.6 211,302 + GFF180 0.43 +0.0 211,302 + GFF180 0.43 -0.9 211,637 + -1.2 211,638 - +0.2 211,740 - GFF181 0.16 -0.7 211,889 + GFF181 0.26 +0.0 211,889 + GFF181 0.26 -0.1 211,889 + GFF181 0.26 +0.3 211,889 + GFF181 0.26 -0.5 211,889 + GFF181 0.26 +0.4 211,889 + GFF181 0.26 -0.5 211,889 + GFF181 0.26 -1.2 211,890 - GFF181 0.26 +0.9 211,890 - GFF181 0.26 +0.8 211,890 - GFF181 0.26 -0.7 212,066 + GFF181 0.39 -0.2 212,066 + GFF181 0.39 +0.4 212,066 + GFF181 0.39 -0.4 212,066 + GFF181 0.39 -0.2 212,066 + GFF181 0.39 -0.3 212,067 - GFF181 0.39 -0.3 212,067 - GFF181 0.39 +0.4 212,067 - GFF181 0.39 +1.8 212,067 - GFF181 0.39 -0.3 212,067 - GFF181 0.39 +2.0 212,067 - GFF181 0.39 +0.8 212,067 - GFF181 0.39 -0.9 212,067 - GFF181 0.39 +2.2 212,067 - GFF181 0.39 -1.2 212,067 - GFF181 0.39 -0.4 212,165 + GFF181 0.45 +0.3 212,165 + GFF181 0.45 -0.7 212,166 - GFF181 0.45 -0.5 212,181 + GFF181 0.46 +1.2 212,181 + GFF181 0.46 +1.2 212,182 - GFF181 0.47 -1.1 212,240 + GFF181 0.50 +0.1 212,303 + GFF181 0.55 -0.4 212,303 + GFF181 0.55 +0.8 212,303 + GFF181 0.55 +0.2 212,304 - GFF181 0.55 -1.0 212,304 - GFF181 0.55 +0.7
Or see this region's nucleotide sequence