Strain Fitness in Variovorax sp. SCN45 around GFF180

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF179 and GFF180 are separated by 5 nucleotidesGFF180 and GFF181 are separated by 14 nucleotides GFF179 - D-mannonate oxidoreductase (EC 1.1.1.57), at 209,652 to 211,154 GFF179 GFF180 - no description, at 211,160 to 211,489 GFF180 GFF181 - Protein of unknown function (DUF1501), at 211,504 to 212,961 GFF181 Position (kb) 211 212Strain fitness (log2 ratio) -2 -1 0 1 2at 210.582 kb on + strand, within GFF179at 210.583 kb on - strand, within GFF179at 210.897 kb on + strand, within GFF179at 210.897 kb on + strand, within GFF179at 210.898 kb on - strand, within GFF179at 210.898 kb on - strand, within GFF179at 210.898 kb on - strand, within GFF179at 210.898 kb on - strand, within GFF179at 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.185 kb on + strandat 211.186 kb on - strandat 211.186 kb on - strandat 211.302 kb on + strand, within GFF180at 211.302 kb on + strand, within GFF180at 211.637 kb on + strandat 211.638 kb on - strandat 211.740 kb on - strand, within GFF181at 211.889 kb on + strand, within GFF181at 211.889 kb on + strand, within GFF181at 211.889 kb on + strand, within GFF181at 211.889 kb on + strand, within GFF181at 211.889 kb on + strand, within GFF181at 211.889 kb on + strand, within GFF181at 211.889 kb on + strand, within GFF181at 211.890 kb on - strand, within GFF181at 211.890 kb on - strand, within GFF181at 211.890 kb on - strand, within GFF181at 212.066 kb on + strand, within GFF181at 212.066 kb on + strand, within GFF181at 212.066 kb on + strand, within GFF181at 212.066 kb on + strand, within GFF181at 212.066 kb on + strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.067 kb on - strand, within GFF181at 212.165 kb on + strand, within GFF181at 212.165 kb on + strand, within GFF181at 212.166 kb on - strand, within GFF181at 212.181 kb on + strand, within GFF181at 212.181 kb on + strand, within GFF181at 212.182 kb on - strand, within GFF181at 212.240 kb on + strand, within GFF181at 212.303 kb on + strand, within GFF181at 212.303 kb on + strand, within GFF181at 212.303 kb on + strand, within GFF181at 212.304 kb on - strand, within GFF181at 212.304 kb on - strand, within GFF181

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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210,582 + GFF179 0.62 +0.1
210,583 - GFF179 0.62 -0.6
210,897 + GFF179 0.83 -1.1
210,897 + GFF179 0.83 +0.0
210,898 - GFF179 0.83 -0.2
210,898 - GFF179 0.83 +1.0
210,898 - GFF179 0.83 -0.1
210,898 - GFF179 0.83 -2.1
211,185 + +0.3
211,185 + +0.2
211,185 + -0.1
211,185 + -1.0
211,185 + -0.5
211,185 + +1.6
211,185 + -0.2
211,185 + -0.4
211,185 + +1.1
211,185 + -0.2
211,185 + -0.5
211,186 - +0.1
211,186 - -0.6
211,302 + GFF180 0.43 +0.0
211,302 + GFF180 0.43 -0.9
211,637 + -1.2
211,638 - +0.2
211,740 - GFF181 0.16 -0.7
211,889 + GFF181 0.26 +0.0
211,889 + GFF181 0.26 -0.1
211,889 + GFF181 0.26 +0.3
211,889 + GFF181 0.26 -0.5
211,889 + GFF181 0.26 +0.4
211,889 + GFF181 0.26 -0.5
211,889 + GFF181 0.26 -1.2
211,890 - GFF181 0.26 +0.9
211,890 - GFF181 0.26 +0.8
211,890 - GFF181 0.26 -0.7
212,066 + GFF181 0.39 -0.2
212,066 + GFF181 0.39 +0.4
212,066 + GFF181 0.39 -0.4
212,066 + GFF181 0.39 -0.2
212,066 + GFF181 0.39 -0.3
212,067 - GFF181 0.39 -0.3
212,067 - GFF181 0.39 +0.4
212,067 - GFF181 0.39 +1.8
212,067 - GFF181 0.39 -0.3
212,067 - GFF181 0.39 +2.0
212,067 - GFF181 0.39 +0.8
212,067 - GFF181 0.39 -0.9
212,067 - GFF181 0.39 +2.2
212,067 - GFF181 0.39 -1.2
212,067 - GFF181 0.39 -0.4
212,165 + GFF181 0.45 +0.3
212,165 + GFF181 0.45 -0.7
212,166 - GFF181 0.45 -0.5
212,181 + GFF181 0.46 +1.2
212,181 + GFF181 0.46 +1.2
212,182 - GFF181 0.47 -1.1
212,240 + GFF181 0.50 +0.1
212,303 + GFF181 0.55 -0.4
212,303 + GFF181 0.55 +0.8
212,303 + GFF181 0.55 +0.2
212,304 - GFF181 0.55 -1.0
212,304 - GFF181 0.55 +0.7

Or see this region's nucleotide sequence