Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1589 and GFF1590 are separated by 34 nucleotides GFF1590 and GFF1591 are separated by 0 nucleotides GFF1591 and GFF1592 overlap by 4 nucleotides
GFF1589 - FIG00765372: hypothetical protein, at 188,115 to 188,687
GFF1589
GFF1590 - FIG057251: Fe-S oxidoreductase, at 188,722 to 189,111
GFF1590
GFF1591 - FIG066100: Diguanylate cyclase (GGDEF domain) with PAS/PAC sensor, at 189,112 to 189,852
GFF1591
GFF1592 - Response regulator/GGDEF/EAL domain protein, at 189,849 to 192,089
GFF1592
Position (kb) 189
190 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 188.142 kb on + strand at 188.142 kb on + strand at 188.142 kb on + strand at 188.142 kb on + strand at 188.142 kb on + strand at 188.143 kb on - strand at 188.143 kb on - strand at 188.143 kb on - strand at 188.143 kb on - strand at 188.187 kb on + strand, within GFF1589 at 188.187 kb on + strand, within GFF1589 at 188.188 kb on - strand, within GFF1589 at 188.188 kb on - strand, within GFF1589 at 188.190 kb on + strand, within GFF1589 at 188.190 kb on + strand, within GFF1589 at 188.191 kb on - strand, within GFF1589 at 188.191 kb on - strand, within GFF1589 at 188.199 kb on + strand, within GFF1589 at 188.200 kb on - strand, within GFF1589 at 188.200 kb on - strand, within GFF1589 at 188.342 kb on + strand, within GFF1589 at 188.425 kb on + strand, within GFF1589 at 188.425 kb on + strand, within GFF1589 at 188.425 kb on + strand, within GFF1589 at 188.426 kb on - strand, within GFF1589 at 188.426 kb on - strand, within GFF1589 at 188.526 kb on + strand, within GFF1589 at 188.667 kb on + strand at 188.668 kb on - strand at 188.685 kb on + strand at 188.686 kb on - strand at 188.717 kb on + strand at 188.717 kb on + strand at 188.734 kb on + strand at 188.735 kb on - strand at 188.735 kb on - strand at 188.735 kb on - strand at 188.812 kb on + strand, within GFF1590 at 188.836 kb on + strand, within GFF1590 at 188.837 kb on - strand, within GFF1590 at 188.837 kb on - strand, within GFF1590 at 188.837 kb on - strand, within GFF1590 at 188.866 kb on + strand, within GFF1590 at 188.866 kb on + strand, within GFF1590 at 188.866 kb on + strand, within GFF1590 at 188.866 kb on + strand, within GFF1590 at 188.867 kb on - strand, within GFF1590 at 188.917 kb on + strand, within GFF1590 at 189.010 kb on + strand, within GFF1590 at 189.011 kb on - strand, within GFF1590 at 189.248 kb on - strand, within GFF1591 at 189.248 kb on - strand, within GFF1591 at 189.676 kb on + strand, within GFF1591 at 189.676 kb on + strand, within GFF1591 at 189.676 kb on + strand, within GFF1591 at 189.676 kb on + strand, within GFF1591 at 189.677 kb on - strand, within GFF1591 at 189.677 kb on - strand, within GFF1591 at 189.677 kb on - strand, within GFF1591 at 189.677 kb on - strand, within GFF1591 at 189.677 kb on - strand, within GFF1591 at 189.763 kb on + strand, within GFF1591 at 189.763 kb on + strand, within GFF1591 at 189.764 kb on - strand, within GFF1591 at 189.787 kb on + strand at 189.787 kb on + strand at 189.787 kb on + strand at 189.787 kb on + strand at 189.787 kb on + strand at 189.787 kb on + strand at 189.787 kb on + strand at 189.787 kb on + strand at 189.787 kb on + strand at 189.788 kb on - strand at 189.788 kb on - strand at 189.788 kb on - strand at 189.788 kb on - strand at 189.788 kb on - strand at 189.788 kb on - strand at 189.788 kb on - strand at 189.788 kb on - strand at 189.788 kb on - strand at 190.012 kb on - strand at 190.212 kb on + strand, within GFF1592 at 190.213 kb on - strand, within GFF1592 at 190.293 kb on + strand, within GFF1592 at 190.294 kb on - strand, within GFF1592 at 190.294 kb on - strand, within GFF1592 at 190.611 kb on + strand, within GFF1592 at 190.611 kb on + strand, within GFF1592 at 190.789 kb on - strand, within GFF1592 at 190.789 kb on - strand, within GFF1592 at 190.789 kb on - strand, within GFF1592 at 190.797 kb on + strand, within GFF1592 at 190.797 kb on + strand, within GFF1592 at 190.798 kb on - strand, within GFF1592 at 190.798 kb on - strand, within GFF1592 at 190.798 kb on - strand, within GFF1592 at 190.798 kb on - strand, within GFF1592
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 188,142 + -1.3 188,142 + -0.2 188,142 + +0.2 188,142 + +0.3 188,142 + +1.2 188,143 - +0.7 188,143 - +0.4 188,143 - +0.5 188,143 - -0.4 188,187 + GFF1589 0.13 -0.6 188,187 + GFF1589 0.13 -1.2 188,188 - GFF1589 0.13 +1.0 188,188 - GFF1589 0.13 +1.3 188,190 + GFF1589 0.13 +0.1 188,190 + GFF1589 0.13 +0.5 188,191 - GFF1589 0.13 +0.3 188,191 - GFF1589 0.13 -0.1 188,199 + GFF1589 0.15 -1.5 188,200 - GFF1589 0.15 -0.4 188,200 - GFF1589 0.15 +3.2 188,342 + GFF1589 0.40 -0.2 188,425 + GFF1589 0.54 -0.0 188,425 + GFF1589 0.54 +0.5 188,425 + GFF1589 0.54 +0.7 188,426 - GFF1589 0.54 +1.7 188,426 - GFF1589 0.54 -1.1 188,526 + GFF1589 0.72 +0.6 188,667 + -2.2 188,668 - +0.7 188,685 + +0.4 188,686 - +1.3 188,717 + -1.6 188,717 + -0.9 188,734 + -0.5 188,735 - +0.6 188,735 - -0.2 188,735 - -0.7 188,812 + GFF1590 0.23 +0.1 188,836 + GFF1590 0.29 -1.7 188,837 - GFF1590 0.29 -0.9 188,837 - GFF1590 0.29 +0.1 188,837 - GFF1590 0.29 -0.1 188,866 + GFF1590 0.37 +0.4 188,866 + GFF1590 0.37 -0.4 188,866 + GFF1590 0.37 -0.1 188,866 + GFF1590 0.37 +1.2 188,867 - GFF1590 0.37 -0.5 188,917 + GFF1590 0.50 +0.5 189,010 + GFF1590 0.74 +0.9 189,011 - GFF1590 0.74 +0.2 189,248 - GFF1591 0.18 +0.2 189,248 - GFF1591 0.18 +0.9 189,676 + GFF1591 0.76 -0.2 189,676 + GFF1591 0.76 +0.5 189,676 + GFF1591 0.76 +0.2 189,676 + GFF1591 0.76 -0.3 189,677 - GFF1591 0.76 +0.5 189,677 - GFF1591 0.76 +0.4 189,677 - GFF1591 0.76 -0.5 189,677 - GFF1591 0.76 +0.6 189,677 - GFF1591 0.76 -0.3 189,763 + GFF1591 0.88 -1.1 189,763 + GFF1591 0.88 +0.0 189,764 - GFF1591 0.88 +0.0 189,787 + -0.1 189,787 + -0.3 189,787 + -0.5 189,787 + -0.6 189,787 + -0.7 189,787 + -1.3 189,787 + +0.0 189,787 + +1.2 189,787 + +0.0 189,788 - -0.5 189,788 - -0.2 189,788 - -0.4 189,788 - -0.6 189,788 - -0.5 189,788 - -0.1 189,788 - -0.1 189,788 - +0.3 189,788 - +1.0 190,012 - +0.5 190,212 + GFF1592 0.16 -0.8 190,213 - GFF1592 0.16 -0.0 190,293 + GFF1592 0.20 +0.1 190,294 - GFF1592 0.20 -0.2 190,294 - GFF1592 0.20 -0.5 190,611 + GFF1592 0.34 +0.1 190,611 + GFF1592 0.34 +0.4 190,789 - GFF1592 0.42 +0.2 190,789 - GFF1592 0.42 +0.7 190,789 - GFF1592 0.42 -0.4 190,797 + GFF1592 0.42 +0.1 190,797 + GFF1592 0.42 +0.3 190,798 - GFF1592 0.42 +0.2 190,798 - GFF1592 0.42 +0.3 190,798 - GFF1592 0.42 -0.6 190,798 - GFF1592 0.42 -0.7
Or see this region's nucleotide sequence