Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF156 and GFF157 overlap by 4 nucleotides GFF157 and GFF158 overlap by 4 nucleotides GFF158 and GFF159 overlap by 13 nucleotides
GFF156 - Glycosyl transferase, group 2 family, at 184,800 to 185,498
GFF156
GFF157 - Methyltransferase type 12, at 185,495 to 186,100
GFF157
GFF158 - LmbE-like protein, at 186,097 to 186,858
GFF158
GFF159 - Acyl-CoA dehydrogenase/oxidase domain protein, at 186,846 to 187,865
GFF159
Position (kb) 186
187 Strain fitness (log2 ratio) -1
0
1
2 at 185.746 kb on - strand, within GFF157 at 185.958 kb on + strand, within GFF157 at 185.958 kb on + strand, within GFF157 at 185.959 kb on - strand, within GFF157 at 185.971 kb on - strand, within GFF157 at 185.971 kb on - strand, within GFF157 at 186.021 kb on + strand, within GFF157 at 186.022 kb on - strand, within GFF157 at 186.042 kb on + strand at 186.043 kb on - strand at 186.043 kb on - strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.063 kb on + strand at 186.064 kb on - strand at 186.064 kb on - strand at 186.064 kb on - strand at 186.078 kb on + strand at 186.078 kb on + strand at 186.116 kb on + strand at 186.116 kb on + strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.117 kb on - strand at 186.847 kb on + strand at 186.848 kb on - strand at 186.848 kb on - strand at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.164 kb on - strand, within GFF159 at 187.303 kb on + strand, within GFF159 at 187.303 kb on + strand, within GFF159 at 187.303 kb on + strand, within GFF159 at 187.303 kb on + strand, within GFF159 at 187.304 kb on - strand, within GFF159 at 187.304 kb on - strand, within GFF159 at 187.304 kb on - strand, within GFF159 at 187.304 kb on - strand, within GFF159 at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 185,746 - GFF157 0.41 -0.8 185,958 + GFF157 0.76 -0.4 185,958 + GFF157 0.76 +0.5 185,959 - GFF157 0.77 -0.0 185,971 - GFF157 0.79 -0.1 185,971 - GFF157 0.79 -1.1 186,021 + GFF157 0.87 +0.0 186,022 - GFF157 0.87 +0.2 186,042 + -0.5 186,043 - -0.6 186,043 - -0.0 186,063 + +0.0 186,063 + +1.0 186,063 + +0.8 186,063 + -0.0 186,064 - -0.1 186,064 - +0.5 186,064 - -0.4 186,078 + -1.5 186,078 + -0.2 186,116 + +0.7 186,116 + -1.1 186,117 - +1.3 186,117 - -0.4 186,117 - -0.0 186,117 - -0.6 186,847 + -0.8 186,848 - -0.1 186,848 - -0.8 187,163 + GFF159 0.31 +2.2 187,163 + GFF159 0.31 -0.3 187,163 + GFF159 0.31 -0.5 187,163 + GFF159 0.31 -1.0 187,163 + GFF159 0.31 +0.6 187,163 + GFF159 0.31 +1.2 187,164 - GFF159 0.31 -0.3 187,303 + GFF159 0.45 +0.2 187,303 + GFF159 0.45 -0.8 187,303 + GFF159 0.45 -0.5 187,303 + GFF159 0.45 +0.3 187,304 - GFF159 0.45 +0.4 187,304 - GFF159 0.45 -0.2 187,304 - GFF159 0.45 -0.5 187,304 - GFF159 0.45 -1.2 187,839 + +0.5 187,839 + -0.2 187,839 + +0.6 187,839 + -0.6 187,839 + -0.3 187,839 + -0.8 187,839 + -0.8 187,839 + -0.5 187,839 + +1.2 187,839 + +0.8 187,839 + +0.3 187,839 + -0.6 187,839 + -1.2 187,839 + +1.0 187,839 + -0.2 187,840 - -0.4 187,840 - +0.6 187,840 - +0.5 187,840 - -0.3 187,840 - -0.5 187,840 - +0.6 187,840 - +0.5 187,840 - -0.5 187,840 - +0.1 187,840 - -0.4 187,840 - -0.9 187,840 - +0.7 187,840 - +0.1 187,840 - +0.1 187,840 - +0.3 187,840 - -0.8 187,840 - -0.4 187,840 - -0.4 187,840 - -0.7 187,840 - -0.8
Or see this region's nucleotide sequence