Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1577 and GFF1578 are separated by 24 nucleotides GFF1578 and GFF1579 are separated by 37 nucleotides GFF1579 and GFF1580 are separated by 33 nucleotides GFF1580 and GFF1581 overlap by 14 nucleotides
GFF1577 - hypothetical protein, at 177,612 to 178,052
GFF1577
GFF1578 - Nitrilotriacetate monooxygenase component B (EC 1.14.13.-), at 178,077 to 178,706
GFF1578
GFF1579 - hypothetical protein, at 178,744 to 179,577
GFF1579
GFF1580 - Mhp operon transcriptional activator, at 179,611 to 180,378
GFF1580
GFF1581 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.52), at 180,365 to 181,114
GFF1581
Position (kb) 178
179
180 Strain fitness (log2 ratio) -1
0
1 at 177.787 kb on - strand, within GFF1577 at 177.882 kb on + strand, within GFF1577 at 177.882 kb on + strand, within GFF1577 at 177.882 kb on + strand, within GFF1577 at 177.883 kb on - strand, within GFF1577 at 177.898 kb on - strand, within GFF1577 at 178.651 kb on - strand at 178.651 kb on - strand at 179.131 kb on + strand, within GFF1579 at 179.132 kb on - strand, within GFF1579 at 179.254 kb on + strand, within GFF1579 at 179.255 kb on - strand, within GFF1579 at 179.255 kb on - strand, within GFF1579 at 179.554 kb on + strand at 179.555 kb on - strand at 179.596 kb on + strand at 179.658 kb on - strand at 179.800 kb on + strand, within GFF1580 at 179.935 kb on + strand, within GFF1580 at 179.957 kb on - strand, within GFF1580 at 180.046 kb on + strand, within GFF1580 at 180.047 kb on - strand, within GFF1580 at 180.181 kb on + strand, within GFF1580 at 180.182 kb on - strand, within GFF1580 at 180.376 kb on + strand at 180.377 kb on - strand at 180.377 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 177,787 - GFF1577 0.40 +0.1 177,882 + GFF1577 0.61 -0.4 177,882 + GFF1577 0.61 -0.4 177,882 + GFF1577 0.61 -0.2 177,883 - GFF1577 0.61 -0.7 177,898 - GFF1577 0.65 +0.2 178,651 - -0.2 178,651 - -0.3 179,131 + GFF1579 0.46 -0.2 179,132 - GFF1579 0.47 +0.7 179,254 + GFF1579 0.61 -1.2 179,255 - GFF1579 0.61 -0.0 179,255 - GFF1579 0.61 -1.3 179,554 + -0.1 179,555 - +0.0 179,596 + -0.3 179,658 - +0.2 179,800 + GFF1580 0.25 -0.3 179,935 + GFF1580 0.42 +0.1 179,957 - GFF1580 0.45 -0.1 180,046 + GFF1580 0.57 +0.1 180,047 - GFF1580 0.57 +0.3 180,181 + GFF1580 0.74 +1.3 180,182 - GFF1580 0.74 -0.3 180,376 + -0.1 180,377 - +0.4 180,377 - -0.5
Or see this region's nucleotide sequence