Strain Fitness in Variovorax sp. SCN45 around GFF1541

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1539 and GFF1540 are separated by 10 nucleotidesGFF1540 and GFF1541 overlap by 11 nucleotidesGFF1541 and GFF1542 are separated by 29 nucleotides GFF1539 - RsbT co-antagonist protein RsbRA, at 137,522 to 138,367 GFF1539 GFF1540 - RsbS, negative regulator of sigma-B, at 138,378 to 138,752 GFF1540 GFF1541 - Anti-sigma B factor RsbT, at 138,742 to 139,146 GFF1541 GFF1542 - Anti-sigma B factor RsbT / Phosphoserine phosphatase RsbX (EC 3.1.3.3), at 139,176 to 140,171 GFF1542 Position (kb) 138 139 140Strain fitness (log2 ratio) -1 0 1at 137.936 kb on + strand, within GFF1539at 137.936 kb on + strand, within GFF1539at 137.936 kb on + strand, within GFF1539at 139.144 kb on + strandat 139.144 kb on + strandat 139.145 kb on - strandat 139.145 kb on - strandat 139.145 kb on - strandat 139.835 kb on - strand, within GFF1542at 139.876 kb on - strand, within GFF1542at 139.876 kb on - strand, within GFF1542at 140.120 kb on + strandat 140.120 kb on + strandat 140.120 kb on + strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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137,936 + GFF1539 0.49 -0.5
137,936 + GFF1539 0.49 -0.5
137,936 + GFF1539 0.49 -0.9
139,144 + -0.3
139,144 + +0.2
139,145 - +0.6
139,145 - -0.6
139,145 - -0.3
139,835 - GFF1542 0.66 -0.8
139,876 - GFF1542 0.70 -0.2
139,876 - GFF1542 0.70 +0.3
140,120 + -0.2
140,120 + +0.0
140,120 + +0.1
140,121 - -0.3
140,121 - -0.8
140,121 - -0.3
140,121 - -0.3
140,121 - +0.3
140,121 - -1.5
140,121 - +0.4
140,121 - +0.3
140,121 - +0.2

Or see this region's nucleotide sequence