Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF148 and GFF150 are separated by 35 nucleotides GFF150 and GFF149 overlap by 2 nucleotides
GFF148 - Transcriptional regulator, LysR family, at 176,590 to 177,483
GFF148
GFF150 - no description, at 177,519 to 177,638
GFF150
GFF149 - Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (EC 2.3.1.164), at 177,637 to 178,797
GFF149
Position (kb) 177
178 Strain fitness (log2 ratio) -2
-1
0
1
2 at 176.537 kb on - strand at 176.559 kb on + strand at 176.560 kb on - strand at 176.560 kb on - strand at 176.560 kb on - strand at 176.560 kb on - strand at 176.999 kb on + strand, within GFF148 at 176.999 kb on + strand, within GFF148 at 176.999 kb on + strand, within GFF148 at 176.999 kb on + strand, within GFF148 at 176.999 kb on + strand, within GFF148 at 177.257 kb on + strand, within GFF148 at 177.258 kb on - strand, within GFF148 at 177.258 kb on - strand, within GFF148 at 177.514 kb on + strand at 177.652 kb on + strand at 177.652 kb on + strand at 177.652 kb on + strand at 177.653 kb on - strand at 177.653 kb on - strand at 177.653 kb on - strand at 177.653 kb on - strand at 177.653 kb on - strand at 177.653 kb on - strand at 177.679 kb on - strand at 177.749 kb on - strand at 177.833 kb on - strand, within GFF149 at 177.833 kb on - strand, within GFF149 at 177.833 kb on - strand, within GFF149 at 177.844 kb on + strand, within GFF149 at 177.844 kb on + strand, within GFF149 at 177.844 kb on + strand, within GFF149 at 177.844 kb on + strand, within GFF149 at 177.844 kb on + strand, within GFF149 at 177.938 kb on - strand, within GFF149 at 178.071 kb on + strand, within GFF149 at 178.071 kb on + strand, within GFF149 at 178.072 kb on - strand, within GFF149 at 178.072 kb on - strand, within GFF149 at 178.072 kb on - strand, within GFF149 at 178.342 kb on + strand, within GFF149 at 178.537 kb on + strand, within GFF149 at 178.537 kb on + strand, within GFF149 at 178.537 kb on + strand, within GFF149 at 178.538 kb on - strand, within GFF149 at 178.538 kb on - strand, within GFF149 at 178.538 kb on - strand, within GFF149 at 178.538 kb on - strand, within GFF149 at 178.538 kb on - strand, within GFF149 at 178.538 kb on - strand, within GFF149
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 176,537 - -0.4 176,559 + -2.5 176,560 - +0.1 176,560 - +0.2 176,560 - +0.7 176,560 - -0.7 176,999 + GFF148 0.46 -1.3 176,999 + GFF148 0.46 +0.6 176,999 + GFF148 0.46 +0.0 176,999 + GFF148 0.46 -0.1 176,999 + GFF148 0.46 +0.9 177,257 + GFF148 0.75 -0.5 177,258 - GFF148 0.75 -0.3 177,258 - GFF148 0.75 +0.5 177,514 + +0.5 177,652 + -0.4 177,652 + +0.2 177,652 + -0.4 177,653 - +0.6 177,653 - +0.1 177,653 - -0.1 177,653 - +0.7 177,653 - +0.2 177,653 - +0.8 177,679 - -0.5 177,749 - -0.7 177,833 - GFF149 0.17 +0.4 177,833 - GFF149 0.17 -0.1 177,833 - GFF149 0.17 -0.9 177,844 + GFF149 0.18 +2.2 177,844 + GFF149 0.18 -0.6 177,844 + GFF149 0.18 -0.3 177,844 + GFF149 0.18 -0.3 177,844 + GFF149 0.18 +0.2 177,938 - GFF149 0.26 +0.6 178,071 + GFF149 0.37 -0.0 178,071 + GFF149 0.37 +0.2 178,072 - GFF149 0.37 +0.3 178,072 - GFF149 0.37 -1.4 178,072 - GFF149 0.37 -0.5 178,342 + GFF149 0.61 -1.0 178,537 + GFF149 0.78 +0.2 178,537 + GFF149 0.78 +0.8 178,537 + GFF149 0.78 +0.6 178,538 - GFF149 0.78 -1.4 178,538 - GFF149 0.78 -0.1 178,538 - GFF149 0.78 -0.5 178,538 - GFF149 0.78 -0.4 178,538 - GFF149 0.78 -0.3 178,538 - GFF149 0.78 +0.2
Or see this region's nucleotide sequence