Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1494 and GFF1495 are separated by 53 nucleotides GFF1495 and GFF1496 overlap by 4 nucleotides GFF1496 and GFF1497 are separated by 38 nucleotides
GFF1494 - BUG/TctC family periplasmic protein, at 87,162 to 88,142
GFF1494
GFF1495 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 88,196 to 89,122
GFF1495
GFF1496 - Demethylmenaquinone methyltransferase-like protein, at 89,119 to 89,808
GFF1496
GFF1497 - BUG/TctC family periplasmic protein, at 89,847 to 90,833
GFF1497
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-2
-1
0
1 at 87.203 kb on - strand at 87.379 kb on + strand, within GFF1494 at 87.397 kb on + strand, within GFF1494 at 87.397 kb on + strand, within GFF1494 at 87.671 kb on - strand, within GFF1494 at 87.671 kb on - strand, within GFF1494 at 87.671 kb on - strand, within GFF1494 at 87.671 kb on - strand, within GFF1494 at 87.826 kb on + strand, within GFF1494 at 87.827 kb on - strand, within GFF1494 at 87.874 kb on + strand, within GFF1494 at 87.874 kb on + strand, within GFF1494 at 87.874 kb on + strand, within GFF1494 at 87.874 kb on + strand, within GFF1494 at 87.875 kb on - strand, within GFF1494 at 88.266 kb on + strand at 88.266 kb on + strand at 88.267 kb on - strand at 88.267 kb on - strand at 88.267 kb on - strand at 88.267 kb on - strand at 88.267 kb on - strand at 88.267 kb on - strand at 89.046 kb on + strand at 89.046 kb on + strand at 89.047 kb on - strand at 89.047 kb on - strand at 89.047 kb on - strand at 89.047 kb on - strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.051 kb on + strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 89.052 kb on - strand at 90.055 kb on + strand, within GFF1497 at 90.115 kb on - strand, within GFF1497
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 87,203 - +1.3 87,379 + GFF1494 0.22 -1.3 87,397 + GFF1494 0.24 -0.5 87,397 + GFF1494 0.24 -0.7 87,671 - GFF1494 0.52 -0.9 87,671 - GFF1494 0.52 +1.4 87,671 - GFF1494 0.52 -0.3 87,671 - GFF1494 0.52 -1.8 87,826 + GFF1494 0.68 +0.3 87,827 - GFF1494 0.68 -0.7 87,874 + GFF1494 0.73 -0.1 87,874 + GFF1494 0.73 -0.6 87,874 + GFF1494 0.73 +0.5 87,874 + GFF1494 0.73 +0.1 87,875 - GFF1494 0.73 +0.2 88,266 + -0.1 88,266 + +0.3 88,267 - -0.1 88,267 - +0.8 88,267 - +0.1 88,267 - -0.4 88,267 - -0.5 88,267 - -0.1 89,046 + -0.4 89,046 + -0.3 89,047 - -0.2 89,047 - -0.4 89,047 - +0.2 89,047 - -0.3 89,051 + -0.4 89,051 + -0.3 89,051 + +1.2 89,051 + +0.2 89,051 + +0.5 89,051 + -0.1 89,051 + -0.8 89,051 + -0.2 89,051 + +0.3 89,051 + +0.2 89,051 + -0.5 89,052 - +0.7 89,052 - +0.3 89,052 - +1.2 89,052 - -0.4 89,052 - -0.5 89,052 - +0.5 89,052 - -0.4 89,052 - +0.4 89,052 - -0.3 89,052 - +0.4 89,052 - +0.7 90,055 + GFF1497 0.21 -0.2 90,115 - GFF1497 0.27 +0.1
Or see this region's nucleotide sequence