Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1307 and GFF1308 are separated by 6 nucleotides GFF1308 and GFF1309 are separated by 4 nucleotides
GFF1307 - Homoserine dehydrogenase (EC 1.1.1.3), at 637,112 to 638,476
GFF1307
GFF1308 - hypothetical protein, at 638,483 to 638,881
GFF1308
GFF1309 - Phosphoglucomutase (EC 5.4.2.2), at 638,886 to 640,541
GFF1309
Position (kb) 638
639 Strain fitness (log2 ratio) -2
-1
0
1
2 at 637.551 kb on - strand, within GFF1307 at 637.551 kb on - strand, within GFF1307 at 637.551 kb on - strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.057 kb on + strand, within GFF1307 at 638.057 kb on + strand, within GFF1307 at 638.057 kb on + strand, within GFF1307 at 638.058 kb on - strand, within GFF1307 at 638.058 kb on - strand, within GFF1307 at 638.231 kb on + strand, within GFF1307 at 638.231 kb on + strand, within GFF1307 at 638.231 kb on + strand, within GFF1307 at 638.232 kb on - strand, within GFF1307 at 638.232 kb on - strand, within GFF1307 at 638.514 kb on + strand at 638.514 kb on + strand at 638.515 kb on - strand at 638.974 kb on + strand at 638.974 kb on + strand at 638.975 kb on - strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 639.230 kb on - strand, within GFF1309 at 639.427 kb on + strand, within GFF1309 at 639.427 kb on + strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.529 kb on + strand, within GFF1309 at 639.529 kb on + strand, within GFF1309 at 639.530 kb on - strand, within GFF1309 at 639.535 kb on + strand, within GFF1309 at 639.535 kb on + strand, within GFF1309 at 639.535 kb on + strand, within GFF1309 at 639.535 kb on + strand, within GFF1309 at 639.536 kb on - strand, within GFF1309 at 639.536 kb on - strand, within GFF1309 at 639.556 kb on + strand, within GFF1309 at 639.557 kb on - strand, within GFF1309 at 639.781 kb on + strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.806 kb on - strand, within GFF1309 at 639.806 kb on - strand, within GFF1309
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 637,551 - GFF1307 0.32 -0.7 637,551 - GFF1307 0.32 -0.4 637,551 - GFF1307 0.32 -2.2 638,054 + GFF1307 0.69 -0.4 638,054 + GFF1307 0.69 -0.1 638,054 + GFF1307 0.69 -0.7 638,054 + GFF1307 0.69 +0.4 638,054 + GFF1307 0.69 +0.5 638,054 + GFF1307 0.69 -0.1 638,054 + GFF1307 0.69 +0.3 638,054 + GFF1307 0.69 +0.7 638,054 + GFF1307 0.69 +0.1 638,055 - GFF1307 0.69 -0.2 638,055 - GFF1307 0.69 -1.9 638,055 - GFF1307 0.69 -1.3 638,055 - GFF1307 0.69 -0.6 638,055 - GFF1307 0.69 +0.4 638,055 - GFF1307 0.69 -0.8 638,055 - GFF1307 0.69 -0.7 638,055 - GFF1307 0.69 -0.4 638,055 - GFF1307 0.69 +0.5 638,057 + GFF1307 0.69 -0.4 638,057 + GFF1307 0.69 +0.3 638,057 + GFF1307 0.69 +1.9 638,058 - GFF1307 0.69 -1.5 638,058 - GFF1307 0.69 -1.1 638,231 + GFF1307 0.82 -0.6 638,231 + GFF1307 0.82 -0.4 638,231 + GFF1307 0.82 +0.5 638,232 - GFF1307 0.82 -0.3 638,232 - GFF1307 0.82 +0.7 638,514 + -1.9 638,514 + +0.1 638,515 - +0.6 638,974 + +0.2 638,974 + +0.3 638,975 - -1.3 638,983 + -1.0 638,983 + -1.4 638,983 + -1.4 638,983 + -1.1 638,983 + +0.3 638,983 + -0.6 638,983 + -0.1 638,984 - +0.2 638,984 - +0.3 638,984 - +1.5 638,984 - -1.0 638,984 - +0.3 638,984 - -2.2 638,984 - -0.7 638,984 - +1.3 638,984 - +1.3 638,984 - -0.5 638,984 - +0.3 639,230 - GFF1309 0.21 -1.3 639,427 + GFF1309 0.33 +0.2 639,427 + GFF1309 0.33 -0.4 639,428 - GFF1309 0.33 +1.0 639,428 - GFF1309 0.33 -0.3 639,428 - GFF1309 0.33 +0.7 639,428 - GFF1309 0.33 +0.5 639,428 - GFF1309 0.33 +0.2 639,428 - GFF1309 0.33 -0.6 639,428 - GFF1309 0.33 +0.0 639,428 - GFF1309 0.33 -0.6 639,428 - GFF1309 0.33 -0.3 639,428 - GFF1309 0.33 +0.9 639,529 + GFF1309 0.39 -0.4 639,529 + GFF1309 0.39 +0.5 639,530 - GFF1309 0.39 -0.5 639,535 + GFF1309 0.39 -0.3 639,535 + GFF1309 0.39 -0.1 639,535 + GFF1309 0.39 -0.2 639,535 + GFF1309 0.39 -0.4 639,536 - GFF1309 0.39 -1.2 639,536 - GFF1309 0.39 -0.0 639,556 + GFF1309 0.40 +0.8 639,557 - GFF1309 0.41 +0.1 639,781 + GFF1309 0.54 -0.2 639,782 - GFF1309 0.54 -0.6 639,782 - GFF1309 0.54 -1.2 639,782 - GFF1309 0.54 -0.2 639,806 - GFF1309 0.56 -0.5 639,806 - GFF1309 0.56 -0.7
Or see this region's nucleotide sequence