Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1298 and GFF1299 are separated by 22 nucleotides GFF1299 and GFF1300 are separated by 113 nucleotides GFF1300 and GFF1301 are separated by 49 nucleotides
GFF1298 - Cell division protein, at 628,732 to 629,835
GFF1298
GFF1299 - DNA ligase (NAD(+)) (EC 6.5.1.2), at 629,858 to 631,942
GFF1299
GFF1300 - hypothetical protein, at 632,056 to 632,472
GFF1300
GFF1301 - Peptide deformylase (EC 3.5.1.88), at 632,522 to 633,061
GFF1301
Position (kb)
629
630
631
632 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 629.052 kb on + strand, within GFF1298 at 629.194 kb on + strand, within GFF1298 at 629.194 kb on + strand, within GFF1298 at 629.194 kb on + strand, within GFF1298 at 629.194 kb on + strand, within GFF1298 at 629.194 kb on + strand, within GFF1298 at 629.194 kb on + strand, within GFF1298 at 629.195 kb on - strand, within GFF1298 at 629.257 kb on + strand, within GFF1298 at 629.257 kb on + strand, within GFF1298 at 629.434 kb on + strand, within GFF1298 at 629.434 kb on + strand, within GFF1298 at 629.770 kb on + strand at 629.770 kb on + strand at 629.770 kb on + strand at 629.833 kb on + strand at 629.833 kb on + strand at 629.833 kb on + strand at 629.834 kb on - strand at 629.834 kb on - strand at 631.940 kb on + strand at 631.941 kb on - strand at 632.007 kb on + strand at 632.007 kb on + strand at 632.007 kb on + strand at 632.007 kb on + strand at 632.007 kb on + strand at 632.007 kb on + strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.008 kb on - strand at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.143 kb on + strand, within GFF1300 at 632.144 kb on - strand, within GFF1300 at 632.144 kb on - strand, within GFF1300 at 632.158 kb on + strand, within GFF1300 at 632.158 kb on + strand, within GFF1300 at 632.158 kb on + strand, within GFF1300 at 632.173 kb on + strand, within GFF1300 at 632.173 kb on + strand, within GFF1300 at 632.173 kb on + strand, within GFF1300 at 632.173 kb on + strand, within GFF1300 at 632.174 kb on - strand, within GFF1300 at 632.174 kb on - strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.176 kb on + strand, within GFF1300 at 632.177 kb on - strand, within GFF1300 at 632.177 kb on - strand, within GFF1300 at 632.177 kb on - strand, within GFF1300 at 632.177 kb on - strand, within GFF1300 at 632.177 kb on - strand, within GFF1300 at 632.177 kb on - strand, within GFF1300 at 632.177 kb on - strand, within GFF1300 at 632.177 kb on - strand, within GFF1300 at 632.218 kb on + strand, within GFF1300 at 632.219 kb on - strand, within GFF1300 at 632.219 kb on - strand, within GFF1300 at 632.219 kb on - strand, within GFF1300 at 632.219 kb on - strand, within GFF1300 at 632.219 kb on - strand, within GFF1300 at 632.219 kb on - strand, within GFF1300 at 632.219 kb on - strand, within GFF1300 at 632.236 kb on + strand, within GFF1300 at 632.236 kb on + strand, within GFF1300 at 632.236 kb on + strand, within GFF1300 at 632.236 kb on + strand, within GFF1300 at 632.236 kb on + strand, within GFF1300 at 632.236 kb on + strand, within GFF1300 at 632.236 kb on + strand, within GFF1300 at 632.237 kb on - strand, within GFF1300 at 632.237 kb on - strand, within GFF1300 at 632.237 kb on - strand, within GFF1300 at 632.239 kb on + strand, within GFF1300 at 632.239 kb on + strand, within GFF1300 at 632.239 kb on + strand, within GFF1300 at 632.239 kb on + strand, within GFF1300 at 632.239 kb on + strand, within GFF1300 at 632.239 kb on + strand, within GFF1300 at 632.239 kb on + strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.240 kb on - strand, within GFF1300 at 632.257 kb on + strand, within GFF1300 at 632.257 kb on + strand, within GFF1300 at 632.257 kb on - strand, within GFF1300 at 632.258 kb on - strand, within GFF1300 at 632.258 kb on - strand, within GFF1300 at 632.258 kb on - strand, within GFF1300 at 632.258 kb on - strand, within GFF1300 at 632.260 kb on + strand, within GFF1300 at 632.260 kb on + strand, within GFF1300 at 632.260 kb on + strand, within GFF1300 at 632.261 kb on - strand, within GFF1300 at 632.275 kb on + strand, within GFF1300 at 632.276 kb on - strand, within GFF1300 at 632.276 kb on - strand, within GFF1300 at 632.278 kb on + strand, within GFF1300 at 632.278 kb on + strand, within GFF1300 at 632.278 kb on + strand, within GFF1300 at 632.278 kb on + strand, within GFF1300 at 632.279 kb on - strand, within GFF1300 at 632.279 kb on - strand, within GFF1300 at 632.279 kb on - strand, within GFF1300 at 632.279 kb on - strand, within GFF1300 at 632.279 kb on - strand, within GFF1300 at 632.279 kb on - strand, within GFF1300 at 632.293 kb on + strand, within GFF1300 at 632.457 kb on + strand at 632.457 kb on + strand at 632.457 kb on + strand at 632.457 kb on + strand at 632.457 kb on + strand at 632.457 kb on + strand at 632.458 kb on - strand at 632.458 kb on - strand at 632.512 kb on + strand at 632.679 kb on + strand, within GFF1301 at 632.679 kb on + strand, within GFF1301 at 632.680 kb on - strand, within GFF1301 at 632.680 kb on - strand, within GFF1301 at 632.680 kb on - strand, within GFF1301 at 632.680 kb on - strand, within GFF1301 at 632.876 kb on + strand, within GFF1301 at 632.877 kb on - strand, within GFF1301 at 632.877 kb on - strand, within GFF1301 at 632.877 kb on - strand, within GFF1301 at 632.879 kb on - strand, within GFF1301 at 632.894 kb on + strand, within GFF1301 at 632.894 kb on + strand, within GFF1301
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 629,052 + GFF1298 0.29 -1.3 629,194 + GFF1298 0.42 -1.3 629,194 + GFF1298 0.42 -0.2 629,194 + GFF1298 0.42 -0.8 629,194 + GFF1298 0.42 +0.7 629,194 + GFF1298 0.42 -0.8 629,194 + GFF1298 0.42 +0.6 629,195 - GFF1298 0.42 +1.1 629,257 + GFF1298 0.48 -0.6 629,257 + GFF1298 0.48 -0.2 629,434 + GFF1298 0.64 -0.9 629,434 + GFF1298 0.64 -0.9 629,770 + +0.2 629,770 + -0.4 629,770 + -0.0 629,833 + +0.1 629,833 + -0.2 629,833 + -0.1 629,834 - -1.2 629,834 - +0.5 631,940 + -1.1 631,941 - -2.4 632,007 + +0.3 632,007 + +1.0 632,007 + -0.7 632,007 + -0.8 632,007 + -0.8 632,007 + +0.4 632,008 - -0.1 632,008 - -0.4 632,008 - +0.1 632,008 - -0.1 632,008 - -0.1 632,008 - +2.6 632,008 - -0.2 632,008 - -1.4 632,008 - -0.5 632,008 - -0.0 632,143 + GFF1300 0.21 -0.3 632,143 + GFF1300 0.21 +1.0 632,143 + GFF1300 0.21 +0.5 632,143 + GFF1300 0.21 -0.2 632,143 + GFF1300 0.21 +0.2 632,143 + GFF1300 0.21 +0.8 632,143 + GFF1300 0.21 -0.2 632,143 + GFF1300 0.21 -0.4 632,143 + GFF1300 0.21 +0.3 632,143 + GFF1300 0.21 +0.7 632,143 + GFF1300 0.21 -0.2 632,144 - GFF1300 0.21 -0.4 632,144 - GFF1300 0.21 -0.5 632,158 + GFF1300 0.24 -0.6 632,158 + GFF1300 0.24 +0.5 632,158 + GFF1300 0.24 -0.1 632,173 + GFF1300 0.28 +0.2 632,173 + GFF1300 0.28 -0.0 632,173 + GFF1300 0.28 +0.1 632,173 + GFF1300 0.28 -0.1 632,174 - GFF1300 0.28 -0.6 632,174 - GFF1300 0.28 +0.4 632,176 + GFF1300 0.29 -0.3 632,176 + GFF1300 0.29 -0.6 632,176 + GFF1300 0.29 -0.2 632,176 + GFF1300 0.29 +0.2 632,176 + GFF1300 0.29 +0.0 632,176 + GFF1300 0.29 -0.3 632,176 + GFF1300 0.29 -0.7 632,176 + GFF1300 0.29 +0.5 632,176 + GFF1300 0.29 -0.1 632,176 + GFF1300 0.29 +0.1 632,176 + GFF1300 0.29 +0.7 632,176 + GFF1300 0.29 -0.2 632,177 - GFF1300 0.29 -0.1 632,177 - GFF1300 0.29 +1.0 632,177 - GFF1300 0.29 +1.7 632,177 - GFF1300 0.29 +0.4 632,177 - GFF1300 0.29 +0.9 632,177 - GFF1300 0.29 -0.0 632,177 - GFF1300 0.29 -1.1 632,177 - GFF1300 0.29 -0.4 632,218 + GFF1300 0.39 -0.2 632,219 - GFF1300 0.39 +0.1 632,219 - GFF1300 0.39 -0.4 632,219 - GFF1300 0.39 -0.9 632,219 - GFF1300 0.39 -0.5 632,219 - GFF1300 0.39 -0.6 632,219 - GFF1300 0.39 -0.8 632,219 - GFF1300 0.39 -0.0 632,236 + GFF1300 0.43 +0.3 632,236 + GFF1300 0.43 -0.4 632,236 + GFF1300 0.43 -0.7 632,236 + GFF1300 0.43 +0.1 632,236 + GFF1300 0.43 -0.2 632,236 + GFF1300 0.43 +0.3 632,236 + GFF1300 0.43 -0.7 632,237 - GFF1300 0.43 +0.1 632,237 - GFF1300 0.43 +0.9 632,237 - GFF1300 0.43 -0.7 632,239 + GFF1300 0.44 +1.1 632,239 + GFF1300 0.44 +1.1 632,239 + GFF1300 0.44 +0.3 632,239 + GFF1300 0.44 -0.0 632,239 + GFF1300 0.44 -0.3 632,239 + GFF1300 0.44 +0.3 632,239 + GFF1300 0.44 -1.9 632,240 - GFF1300 0.44 +0.8 632,240 - GFF1300 0.44 -0.9 632,240 - GFF1300 0.44 +0.3 632,240 - GFF1300 0.44 +0.8 632,240 - GFF1300 0.44 -0.3 632,240 - GFF1300 0.44 -0.1 632,240 - GFF1300 0.44 -0.4 632,240 - GFF1300 0.44 -0.6 632,240 - GFF1300 0.44 +0.0 632,257 + GFF1300 0.48 +0.5 632,257 + GFF1300 0.48 -0.3 632,257 - GFF1300 0.48 -0.3 632,258 - GFF1300 0.48 +0.9 632,258 - GFF1300 0.48 -0.1 632,258 - GFF1300 0.48 +0.0 632,258 - GFF1300 0.48 -1.2 632,260 + GFF1300 0.49 +0.6 632,260 + GFF1300 0.49 -0.3 632,260 + GFF1300 0.49 -0.2 632,261 - GFF1300 0.49 -0.7 632,275 + GFF1300 0.53 +1.7 632,276 - GFF1300 0.53 -1.0 632,276 - GFF1300 0.53 -1.4 632,278 + GFF1300 0.53 -0.3 632,278 + GFF1300 0.53 +0.5 632,278 + GFF1300 0.53 +0.4 632,278 + GFF1300 0.53 +0.1 632,279 - GFF1300 0.53 -0.2 632,279 - GFF1300 0.53 -0.2 632,279 - GFF1300 0.53 -1.0 632,279 - GFF1300 0.53 +0.6 632,279 - GFF1300 0.53 -1.1 632,279 - GFF1300 0.53 -0.2 632,293 + GFF1300 0.57 -1.0 632,457 + -0.8 632,457 + +0.4 632,457 + -0.3 632,457 + +0.1 632,457 + -0.1 632,457 + +0.5 632,458 - +0.3 632,458 - -0.8 632,512 + +0.3 632,679 + GFF1301 0.29 -0.3 632,679 + GFF1301 0.29 +0.2 632,680 - GFF1301 0.29 -0.7 632,680 - GFF1301 0.29 +1.1 632,680 - GFF1301 0.29 -1.0 632,680 - GFF1301 0.29 +0.6 632,876 + GFF1301 0.66 +0.3 632,877 - GFF1301 0.66 -1.9 632,877 - GFF1301 0.66 -0.4 632,877 - GFF1301 0.66 -0.9 632,879 - GFF1301 0.66 +0.5 632,894 + GFF1301 0.69 -0.3 632,894 + GFF1301 0.69 -0.4
Or see this region's nucleotide sequence