Strain Fitness in Variovorax sp. SCN45 around GFF1233

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1231 and GFF1232 are separated by 34 nucleotidesGFF1232 and GFF1233 are separated by 31 nucleotidesGFF1233 and GFF1234 overlap by 4 nucleotidesGFF1234 and GFF1235 are separated by 102 nucleotides GFF1231 - FOG: WD40-like repeat, at 551,714 to 552,379 GFF1231 GFF1232 - Uncharacterized protein conserved in bacteria, at 552,414 to 553,199 GFF1232 GFF1233 - Arginine/ornithine antiporter ArcD, at 553,231 to 553,875 GFF1233 GFF1234 - Peptide-methionine (S)-S-oxide reductase MsrA (EC 1.8.4.11), at 553,872 to 554,573 GFF1234 GFF1235 - Methionine sulfoxide reductase-associated methionine-rich protein, at 554,676 to 554,942 GFF1235 Position (kb) 553 554Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 552.315 kb on - strandat 552.429 kb on - strandat 552.588 kb on + strand, within GFF1232at 552.588 kb on + strand, within GFF1232at 552.589 kb on - strand, within GFF1232at 552.589 kb on - strand, within GFF1232at 552.589 kb on - strand, within GFF1232at 552.589 kb on - strand, within GFF1232at 552.711 kb on + strand, within GFF1232at 552.711 kb on + strand, within GFF1232at 552.711 kb on + strand, within GFF1232at 552.712 kb on - strand, within GFF1232at 552.712 kb on - strand, within GFF1232at 552.712 kb on - strand, within GFF1232at 552.712 kb on - strand, within GFF1232at 552.712 kb on - strand, within GFF1232at 552.712 kb on - strand, within GFF1232at 552.712 kb on - strand, within GFF1232at 552.712 kb on - strand, within GFF1232at 552.725 kb on + strand, within GFF1232at 552.924 kb on + strand, within GFF1232at 552.925 kb on - strand, within GFF1232at 552.925 kb on - strand, within GFF1232at 552.925 kb on - strand, within GFF1232at 552.925 kb on - strand, within GFF1232at 552.925 kb on - strand, within GFF1232at 552.925 kb on - strand, within GFF1232at 552.981 kb on + strand, within GFF1232at 552.981 kb on + strand, within GFF1232at 552.981 kb on + strand, within GFF1232at 552.982 kb on - strand, within GFF1232at 552.982 kb on - strand, within GFF1232at 553.010 kb on + strand, within GFF1232at 553.010 kb on + strand, within GFF1232at 553.010 kb on + strand, within GFF1232at 553.010 kb on + strand, within GFF1232at 553.010 kb on + strand, within GFF1232at 553.010 kb on + strand, within GFF1232at 553.011 kb on - strand, within GFF1232at 553.011 kb on - strand, within GFF1232at 553.011 kb on - strand, within GFF1232at 553.014 kb on + strand, within GFF1232at 553.014 kb on + strand, within GFF1232at 553.014 kb on + strand, within GFF1232at 553.014 kb on + strand, within GFF1232at 553.014 kb on + strand, within GFF1232at 553.014 kb on + strand, within GFF1232at 553.015 kb on - strand, within GFF1232at 553.056 kb on + strand, within GFF1232at 553.057 kb on - strand, within GFF1232at 553.380 kb on - strand, within GFF1233at 553.915 kb on + strandat 553.915 kb on + strandat 553.916 kb on - strandat 553.916 kb on - strandat 553.916 kb on - strandat 553.916 kb on - strandat 553.916 kb on - strandat 553.975 kb on + strand, within GFF1234at 553.976 kb on - strand, within GFF1234at 553.976 kb on - strand, within GFF1234at 554.002 kb on + strand, within GFF1234at 554.003 kb on - strand, within GFF1234at 554.003 kb on - strand, within GFF1234at 554.003 kb on - strand, within GFF1234at 554.015 kb on - strand, within GFF1234at 554.075 kb on - strand, within GFF1234at 554.075 kb on - strand, within GFF1234at 554.104 kb on + strand, within GFF1234at 554.104 kb on + strand, within GFF1234at 554.104 kb on + strand, within GFF1234at 554.105 kb on - strand, within GFF1234at 554.105 kb on - strand, within GFF1234at 554.105 kb on - strand, within GFF1234at 554.105 kb on - strand, within GFF1234at 554.105 kb on - strand, within GFF1234at 554.158 kb on + strand, within GFF1234at 554.158 kb on + strand, within GFF1234at 554.221 kb on + strand, within GFF1234at 554.222 kb on - strand, within GFF1234at 554.242 kb on + strand, within GFF1234at 554.263 kb on + strand, within GFF1234at 554.264 kb on - strand, within GFF1234at 554.264 kb on - strand, within GFF1234at 554.264 kb on - strand, within GFF1234at 554.347 kb on + strand, within GFF1234at 554.518 kb on + strandat 554.677 kb on + strandat 554.677 kb on + strandat 554.678 kb on - strandat 554.678 kb on - strandat 554.678 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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552,315 - -0.2
552,429 - -0.2
552,588 + GFF1232 0.22 +2.3
552,588 + GFF1232 0.22 -0.4
552,589 - GFF1232 0.22 +0.2
552,589 - GFF1232 0.22 -0.6
552,589 - GFF1232 0.22 +0.4
552,589 - GFF1232 0.22 +0.7
552,711 + GFF1232 0.38 -0.0
552,711 + GFF1232 0.38 -0.1
552,711 + GFF1232 0.38 +1.6
552,712 - GFF1232 0.38 -0.9
552,712 - GFF1232 0.38 -0.0
552,712 - GFF1232 0.38 +0.4
552,712 - GFF1232 0.38 -0.3
552,712 - GFF1232 0.38 +0.4
552,712 - GFF1232 0.38 -0.8
552,712 - GFF1232 0.38 -0.1
552,712 - GFF1232 0.38 -3.0
552,725 + GFF1232 0.40 -1.3
552,924 + GFF1232 0.65 -0.7
552,925 - GFF1232 0.65 -0.1
552,925 - GFF1232 0.65 -0.1
552,925 - GFF1232 0.65 -1.9
552,925 - GFF1232 0.65 -0.5
552,925 - GFF1232 0.65 +1.1
552,925 - GFF1232 0.65 +0.4
552,981 + GFF1232 0.72 -1.5
552,981 + GFF1232 0.72 +1.3
552,981 + GFF1232 0.72 -1.1
552,982 - GFF1232 0.72 +0.3
552,982 - GFF1232 0.72 -0.8
553,010 + GFF1232 0.76 +0.5
553,010 + GFF1232 0.76 -0.6
553,010 + GFF1232 0.76 -1.5
553,010 + GFF1232 0.76 -0.1
553,010 + GFF1232 0.76 -0.5
553,010 + GFF1232 0.76 -0.9
553,011 - GFF1232 0.76 +1.0
553,011 - GFF1232 0.76 +0.3
553,011 - GFF1232 0.76 +0.2
553,014 + GFF1232 0.76 -0.6
553,014 + GFF1232 0.76 +0.4
553,014 + GFF1232 0.76 -2.9
553,014 + GFF1232 0.76 +0.3
553,014 + GFF1232 0.76 +0.1
553,014 + GFF1232 0.76 -1.3
553,015 - GFF1232 0.76 -1.9
553,056 + GFF1232 0.82 +0.7
553,057 - GFF1232 0.82 -0.2
553,380 - GFF1233 0.23 +0.3
553,915 + -1.1
553,915 + +0.4
553,916 - +0.1
553,916 - -0.0
553,916 - -0.4
553,916 - -0.6
553,916 - -0.4
553,975 + GFF1234 0.15 -0.6
553,976 - GFF1234 0.15 -0.0
553,976 - GFF1234 0.15 -0.6
554,002 + GFF1234 0.19 -0.4
554,003 - GFF1234 0.19 +0.1
554,003 - GFF1234 0.19 -0.0
554,003 - GFF1234 0.19 +0.9
554,015 - GFF1234 0.20 +0.7
554,075 - GFF1234 0.29 -0.2
554,075 - GFF1234 0.29 -0.7
554,104 + GFF1234 0.33 -0.4
554,104 + GFF1234 0.33 -0.4
554,104 + GFF1234 0.33 -0.4
554,105 - GFF1234 0.33 +0.6
554,105 - GFF1234 0.33 +0.1
554,105 - GFF1234 0.33 -0.1
554,105 - GFF1234 0.33 +0.2
554,105 - GFF1234 0.33 -0.6
554,158 + GFF1234 0.41 +0.0
554,158 + GFF1234 0.41 -0.6
554,221 + GFF1234 0.50 +0.4
554,222 - GFF1234 0.50 -0.3
554,242 + GFF1234 0.53 -0.6
554,263 + GFF1234 0.56 +0.4
554,264 - GFF1234 0.56 +0.8
554,264 - GFF1234 0.56 +1.0
554,264 - GFF1234 0.56 +0.4
554,347 + GFF1234 0.68 -0.3
554,518 + -0.9
554,677 + +0.8
554,677 + -0.3
554,678 - -1.4
554,678 - -0.7
554,678 - -0.5

Or see this region's nucleotide sequence