Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1199 and GFF1200 overlap by 4 nucleotides GFF1200 and GFF1201 are separated by 5 nucleotides GFF1201 and GFF1202 are separated by 42 nucleotides
GFF1199 - Uncharacterized protein similar to glutamate-1-semialdehyde aminotransferase, at 517,418 to 518,728
GFF1199
GFF1200 - Thioredoxin reductase (EC 1.8.1.9), at 518,725 to 519,744
GFF1200
GFF1201 - 4Fe-4S dicluster fused with DUF3470, at 519,750 to 520,079
GFF1201
GFF1202 - Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4), at 520,122 to 521,459
GFF1202
Position (kb) 518
519
520 Strain fitness (log2 ratio) -2
-1
0
1 at 517.893 kb on - strand, within GFF1199 at 517.893 kb on - strand, within GFF1199 at 517.967 kb on + strand, within GFF1199 at 517.967 kb on + strand, within GFF1199 at 517.968 kb on - strand, within GFF1199 at 517.968 kb on - strand, within GFF1199 at 518.028 kb on + strand, within GFF1199 at 518.028 kb on + strand, within GFF1199 at 518.029 kb on - strand, within GFF1199 at 518.029 kb on - strand, within GFF1199 at 518.219 kb on + strand, within GFF1199 at 518.219 kb on + strand, within GFF1199 at 518.219 kb on + strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.220 kb on - strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.597 kb on + strand, within GFF1199 at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.598 kb on - strand at 518.660 kb on + strand at 518.660 kb on + strand at 518.661 kb on - strand at 518.661 kb on - strand at 518.693 kb on + strand at 518.726 kb on + strand at 518.727 kb on - strand at 520.123 kb on + strand at 520.124 kb on - strand at 520.384 kb on + strand, within GFF1202 at 520.384 kb on + strand, within GFF1202 at 520.385 kb on - strand, within GFF1202 at 520.385 kb on - strand, within GFF1202 at 520.385 kb on - strand, within GFF1202 at 520.385 kb on - strand, within GFF1202
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 517,893 - GFF1199 0.36 +0.5 517,893 - GFF1199 0.36 -0.3 517,967 + GFF1199 0.42 -0.9 517,967 + GFF1199 0.42 -0.9 517,968 - GFF1199 0.42 -0.2 517,968 - GFF1199 0.42 +0.3 518,028 + GFF1199 0.47 -2.6 518,028 + GFF1199 0.47 -0.4 518,029 - GFF1199 0.47 -0.7 518,029 - GFF1199 0.47 +0.9 518,219 + GFF1199 0.61 -0.5 518,219 + GFF1199 0.61 -1.3 518,219 + GFF1199 0.61 -0.2 518,220 - GFF1199 0.61 -0.3 518,220 - GFF1199 0.61 -0.1 518,220 - GFF1199 0.61 +0.6 518,220 - GFF1199 0.61 -0.5 518,220 - GFF1199 0.61 -0.1 518,220 - GFF1199 0.61 -1.4 518,220 - GFF1199 0.61 +0.8 518,220 - GFF1199 0.61 -0.3 518,220 - GFF1199 0.61 -0.7 518,220 - GFF1199 0.61 -1.1 518,220 - GFF1199 0.61 +1.3 518,597 + GFF1199 0.90 -0.3 518,597 + GFF1199 0.90 -0.2 518,597 + GFF1199 0.90 +0.2 518,597 + GFF1199 0.90 -0.7 518,597 + GFF1199 0.90 -0.4 518,597 + GFF1199 0.90 -0.6 518,597 + GFF1199 0.90 +0.1 518,597 + GFF1199 0.90 -0.4 518,597 + GFF1199 0.90 -0.5 518,597 + GFF1199 0.90 +0.1 518,597 + GFF1199 0.90 -0.6 518,597 + GFF1199 0.90 +0.5 518,598 - +0.8 518,598 - +0.8 518,598 - -0.7 518,598 - +0.2 518,598 - -0.3 518,598 - -0.8 518,598 - -0.3 518,598 - +1.3 518,598 - +0.1 518,598 - +0.5 518,598 - -0.0 518,598 - +1.0 518,598 - +0.2 518,598 - +0.3 518,660 + -0.5 518,660 + -0.3 518,661 - +0.3 518,661 - +1.3 518,693 + -0.5 518,726 + -0.3 518,727 - +0.0 520,123 + +1.3 520,124 - -0.3 520,384 + GFF1202 0.20 -0.6 520,384 + GFF1202 0.20 -2.2 520,385 - GFF1202 0.20 -0.6 520,385 - GFF1202 0.20 -0.7 520,385 - GFF1202 0.20 -1.8 520,385 - GFF1202 0.20 -0.8
Or see this region's nucleotide sequence